Design and Characterization of a Fast 16-Source 64-Detector Time-Resolved System for Functional NIR Studies

2005 ◽  
Author(s):  
Davide Contini ◽  
Antonio Pifferi ◽  
Lorenzo Spinelli ◽  
Alessandro Torricelli ◽  
Rinaldo Cubeddu
Keyword(s):  
Time Resolved

scholarly journals Characterization of a Novel FRET Probe in a Crowded Environment using Time-Resolved Anisotropy

2017 ◽  
Vol 112 (3) ◽  
pp. 346a
Author(s):  
Hannah Leopold ◽  
Megan Currie ◽  
Jacob Schwarz ◽  
Arnold J. Boersma ◽  
Erin D. Sheets ◽  
...  
Keyword(s):  
Time Resolved ◽  
Crowded Environment

scholarly journals KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements

2021 ◽  
Author(s):  
Diana Spiegelberg ◽  
Jonas Stenberg ◽  
Pascale Richalet ◽  
Marc Vanhove
Keyword(s):  
Flow Cytometry ◽  
Live Cells ◽  
Time Resolved ◽  
Surface Receptors ◽  
Full Characterization ◽  
End Point ◽  
Titration Curves ◽  
Kinetics Of

AbstractDesign of next-generation therapeutics comes with new challenges and emulates technology and methods to meet them. Characterizing the binding of either natural ligands or therapeutic proteins to cell-surface receptors, for which relevant recombinant versions may not exist, represents one of these challenges. Here we report the characterization of the interaction of five different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate target receptors using LigandTracer. The method offers the advantage of being performed on live cells, alleviating the need for a recombinant source of the receptor. Furthermore, time-resolved measurements, in addition to allowing the determination of the affinity of the studied drug to its target, give access to the binding kinetics thereby providing a full characterization of the system. In this study, we also compared time-resolved LigandTracer data with end-point KD determination from flow cytometry experiments and hypothesize that discrepancies between these two approaches, when they exist, generally come from flow cytometry titration curves being acquired prior to full equilibration of the system. Our data, however, show that knowledge of the kinetics of the interaction allows to reconcile the data obtained by flow cytometry and LigandTracer and demonstrate the complementarity of these two methods.

scholarly journals Time-Resolved Spectroscopic Study of N,N-Di(4-bromo)nitrenium Ions in Selected Solutions

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3182 ◽  
Author(s):  
Lili Du ◽  
Xin Lan ◽  
Zhiping Yan ◽  
Ruixue Zhu ◽  
David Phillips
Keyword(s):  
Transient Absorption ◽  
Direct Detection ◽  
Reaction Pathway ◽  
Addition Reactions ◽  
Time Resolved ◽  
Nitrenium Ions ◽  
Future Studies ◽  
Nitrenium Ion ◽  
Design Time

Nitrenium ions are important reactive intermediates in chemistry and biology. In this work, femtosecond and nanosecond transient absorption (fs-TA and ns-TA) along with nanosecond time-resolved resonance Raman (ns-TR3) experiments were employed to examine the photochemical pathways of N-(4,4′-dibromodiphenylamino)-2,4,6-trimethylpyridinium BF4− (salt (DN) from just absorption of a photon of light to the production of the important N,N-di(4-bromophenyl)nitrenium ion 2. In acetonitrile (MeCN), the formation of halogenated diarylnitrenium ion 2 was observed within 4 ps, showing the vibrational spectra with strong intensity. The nucleophilic adduct reaction of ion 2 with H2O was also examined in aqueous solutions. The direct detection of the unique ortho adduct intermediate 3 shows that there is an efficient and exclusive reaction pathway for 2 with H2O. The results shown in this paper give new characterization of 2, which can be used to design time-resolved spectroscopy investigations of covalent addition reactions of nitrenium ions with other molecules in future studies.

Uncertainty analysis of time resolved transmittance characterization of solid tissue phantoms

2010 ◽  
Author(s):  
Jean-Pierre Bouchard ◽  
Israël Veilleux ◽  
Isabelle Noiseux ◽  
Sébastien Leclair ◽  
Rym Jedidi ◽  
...  
Keyword(s):  
Uncertainty Analysis ◽  
Time Resolved ◽  
Solid Tissue ◽  
Tissue Phantoms

Time-resolved spectrometry for the characterization of a reference field for pulsed radiation

2012 ◽  
Vol 7 (10) ◽  
pp. T10002-T10002
Author(s):  
P Sievers ◽  
J Klammer ◽  
O Hupe ◽  
T Michel ◽  
G Anton
Keyword(s):  
Reference Field ◽  
Time Resolved

Time-resolved chemical characterization of aerosol particles down to 6 nm diameter in Stockton, California

2013 ◽  
Author(s):  
Arantza Eiguren-Fernandez ◽  
Gregory Lewis ◽  
Steven Spielman ◽  
Susanne Hering
Keyword(s):  
Aerosol Particles ◽  
Chemical Characterization ◽  
Time Resolved

Diagnosis and characterization of pulmonary sequestration using dynamic time-resolved magnetic resonance angiography

2008 ◽  
Vol 63 (8) ◽  
pp. 913-917 ◽  
Author(s):  
D.J. Tuite ◽  
C. Francois ◽  
K. Dill ◽  
T.J. Carroll ◽  
T. Grant ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Angiography ◽  
Pulmonary Sequestration ◽  
Time Resolved ◽  
Dynamic Time
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