Tracking of micrometer-sized objects with high-numerical aperture lensless digital holographic microscopy

Author(s):  
John F. Restrepo ◽  
J. Garcia-Sucerquia
2015 ◽  
Vol 54 (32) ◽  
pp. 9540 ◽  
Author(s):  
N. Verrier ◽  
D. Donnarumma ◽  
G. Tessier ◽  
M. Gross

2013 ◽  
Vol 404 ◽  
pp. 490-494
Author(s):  
Han Yen Tu ◽  
Yueh Long Lee ◽  
Chau Jern Cheng

This work presents a close-packed synthetic aperture method for superresolution imaging in digital holographic microscopy. The superresolution imaging technique is based on beam-rotation approach to collect the different bandpass spectrum of the sample. The close-packed information from synthetic aperture process can be used to enhance the reconstructed imaging resolution under low-numerical-aperture microscope objective. Simulated and experimental results show the characteristics of the close-packed synthetic aperture and the influence on superresolution imaging.


Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 264
Author(s):  
Xin Fan ◽  
John J. Healy ◽  
Kevin O’Dwyer ◽  
Julianna Winnik ◽  
Bryan M. Hennelly

Traditional microscopy provides only for a small set of magnifications using a finite set of microscope objectives. Here, a novel architecture is proposed for quantitative phase microscopy that requires only a simple adaptation of the traditional off-axis digital holographic microscope. The architecture has the key advantage of continuously variable magnification, resolution, and Field-of-View, by simply moving the sample. The method is based on combining the principles of traditional off-axis digital holographic microscopy and Gabor microscopy, which uses a diverging spherical wavefield for magnification. We present a proof-of-concept implementation and ray-tracing is used to model the magnification, Numerical Aperture, and Field-of-View as a function of sample position. Experimental results are presented using a micro-lens array and shortcomings of the method are highlighted for future work; in particular, the problem of aberration is highlighted, which results from imaging far from the focal plane of the infinity corrected microscope objective.


Sign in / Sign up

Export Citation Format

Share Document