scholarly journals Augmented line-scan focal modulation microscopy for multi-dimensional imaging of zebrafish heart in vivo

2017 ◽  
Vol 8 (12) ◽  
pp. 5698 ◽  
Author(s):  
Shilpa Pant ◽  
Yubo Duan ◽  
Fei Xiong ◽  
Nanguang Chen
Keyword(s):  
Line Scan ◽  
Zebrafish Heart

scholarly journals In vivo cardiac reprogramming contributes to zebrafish heart regeneration

Nature ◽  
2013 ◽  
Vol 498 (7455) ◽  
pp. 497-501 ◽  
Author(s):  
Ruilin Zhang ◽  
Peidong Han ◽  
Hongbo Yang ◽  
Kunfu Ouyang ◽  
Derek Lee ◽  
...  
Keyword(s):  
Heart Regeneration ◽  
Zebrafish Heart

scholarly journals In Vivo Wall Shear Measurements within the Developing Zebrafish Heart

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e75722 ◽  
Author(s):  
R. Aidan Jamison ◽  
Chaminda R. Samarage ◽  
Robert J. Bryson-Richardson ◽  
Andreas Fouras
Keyword(s):  
Wall Shear ◽  
Zebrafish Heart

Diffusion Tensor Imaging and 3D Tractography of the Cervical Spinal Cord Using the ECG-Gated Line-Scan Technique

2007 ◽  
Vol 20 (5) ◽  
pp. 574-579 ◽  
Author(s):  
M. Hori ◽  
K. Ishigame ◽  
S. Aoki ◽  
H. Kumagai ◽  
T. Araki
Keyword(s):  
Spinal Cord ◽  
Clinical Symptoms ◽  
Diffusion Tensor ◽  
Cervical Spinal Cord ◽  
Three Dimensional ◽  
Acquisition Time ◽  
Clinical Use ◽  
Ecg Gating ◽  
Line Scan

Diffusion tensor (DT) magnetic resonance (MR) imaging in addition to conventional MR images provide valuable information on the brain. This study compared line scan DT imaging with and without the ECG-gating technique to estimate clinical usefulness of the line scan diffusion tensor image (LSDTI) with ECG-gating in evaluating spinal cord diseases in vivo. First, five healthy volunteers participated in the comparison study. LSDWI was performed in three to five sagittal sections with a pulsed-field-gradient diffusion preparation pulse employing two different b-values (0 and 700 s/mm2) along six directions. Apparent diffusion coefficient (ADC) maps and fractional anisotropy (FA) were calculated and three-dimensional tract reconstruction and color schemes of the spinal cord were obtained. Image quality and the acquisition time of each LSDTI were compared. Second, LSDTI with ECG-gating was performed in eighteen patients with cervical spinal cord disorders and evaluated by two neuroradiologists. Images with the ECG-gated technique were all superior to those without ECG—gating. Mean extended time for LSDTI with ECG-gating was approximately two minutes. In clinical use, the ADC and FA of spinal cord in patients with cervical spondylotic myelopathy statically changed. Moreover, demonstration of fibers was correlated with clinical symptoms. ECG-gating technique is preferable to LSDTI. The ADC and FA measurements and 3D fiber tracking of LSDTI with ECG-gating are promising methods to estimate cervical spinal cord pathology in clinical use.

scholarly journals Fast volumetric imaging with line-scan confocal microscopy by an electro-tunable lens

2021 ◽  
Author(s):  
Khuong Duy Mac ◽  
Muhammad Mohsin Qureshi ◽  
Myeongsu Na ◽  
Sunghoe Chang ◽  
Hyuk-Sang Kwon ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
High Performance ◽  
Imaging System ◽  
Ex Vivo ◽  
Resonant Mode ◽  
Biological Tissues ◽  
Line Scan ◽  
Rapid Acquisition ◽  
Tunable Lens

AbstractIn microscopic imaging of biological tissues, particularly real-time visualization of neuronal activities, rapid acquisition of volumetric images poses a prominent challenge. Typically, two-dimensional (2D) microscopy can be devised into an imaging system with 3D capability using any varifocal lens. Despite the conceptual simplicity, such an upgrade yet requires additional, complicated device components and suffers a reduced acquisition rate, which is critical to document neuronal dynamics properly. In this study, we implemented an electro-tunable lens (ETL) in the line-scan confocal microscopy, enabling the volumetric acquisition at the rate of 20 frames per second with the maximum volume of interest of 315 × 315 × 80 μm3. The axial extent of point-spread-function (PSF) was 17.6 ± 1.6 μm and 90.4 ± 2.1 μm with the ETL operating in either stationary or resonant mode, respectively, revealing significant depth elongation by the resonant mode ETL microscopy. We further demonstrated the utilities of the ETL system by volume imaging of cleared mouse brain ex vivo samples and in vivo brains. The current study foregrounds the successful application of resonant ETL for constructing a basis for a high-performance 3D line-scan confocal microscopy system, which will enhance our understanding of various dynamic biological processes.

scholarly journals Line Scan Spatial Speckle Contrast Imaging and Its Application in Blood Flow Imaging

2021 ◽  
Vol 11 (22) ◽  
pp. 10969
Author(s):  
E Du ◽  
Shuhao Shen ◽  
Anqi Qiu ◽  
Nanguang Chen
Keyword(s):  
Blood Flow ◽  
Laser Speckle ◽  
Experimental Results ◽  
Chick Embryos ◽  
Contrast Imaging ◽  
Laser Speckle Imaging ◽  
Speckle Imaging ◽  
Speckle Contrast ◽  
Line Scan

Laser speckle imaging has been an indispensable tool for visualizing blood flow in biomedical applications. We proposed a novel design of the laser speckle imaging system, which combines confocal illumination and detection with various speckle analysis methods. The system can be operated by three imaging modes. One is surface illumination laser speckle contrast imaging (SI-LSCI) and the other two are line scan temporal speckle contrast imaging (LS-TSCI) and line scan spatial speckle contrast imaging (LS-SSCI). The experimental results of flow phantoms have validated the mixture model, which combines the Lorentzian and Gaussian models to describe the simultaneous existence of both Brownian motions and ordered flow. Our experimental results of in vivo chick embryos demonstrate that LS-SSCI maintains high temporal resolution and is less affected by motion artifacts. LS-SSCI can provide better image quality for in vivo imaging blood chick embryos than LS-TSCI. Furthermore, the experiential results present that LS-SSCI can detect and quantify the blood flow change during vascular clipping, and shows great potential in diagnosing vascular diseases, such as angiosclerosis, angiostenosis, or angiemphraxis.

Abstract P303: Development Of Casper/myl7/annexin-5 Transparent Transgenic In-vivo Zebrafish Model To Study The Cardiomyocyte Function

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Surendra K Rajpurohit ◽  
Aaron Gopal ◽  
May Y Mon ◽  
Nikhil Patel ◽  
Vishal Arora
Keyword(s):  
Regulatory Gene ◽  
Fluorescent Microscopy ◽  
Confocal Imaging ◽  
Transgenic Zebrafish ◽  
Heart Cells ◽  
Zebrafish Model ◽  
Transgenic Expression ◽  
Transgenic Progeny ◽  
Zebrafish Heart

The Zebrafish provided an excellent platform to study the genetic and molecular approach ofcardiac research. Zebrafish heart cells similar to human heart cells at the molecular level anddetermine gene functions that control cardiac function and dysfunction. In zebrafish heart, myl7is myosin 7 gene and identified as a regulatory gene orthologs to human MYL7. In the heart,Annexin5 activities contribute to cardiomyocyte dedifferentiation, proliferation and epicardial injuryresponses which leads to cardiac cell death by apoptosis and narcosis pathways. We aredeveloping annexin-5 activity in the cardiovascular function under normal and in metabolicaberration by generating homozygous Casper/ myl7:RFP; annexin-5:YFP transgenic zebrafish.By developing Casper/myl7/Annexin-5 transparent transgenic zebrafish model, we establish time-lapse in-vivo confocal microscopy to study of cellular phenotype/pathologies of thecardiomyocytes over time in newly developed strain to quantify changes in cardiomyocytemorphology and function overtime, comparing control and cardiac injury and cardio-oncologymodels. Transgenic zebrafish has normal type skin pigmentation background. In zebrafish,tracking of transgenic reporter activity in in-vivo is only possible in transparent stage. To maintaintransparency throughout the life, these strains crossbred with the skin transparent mutant Casper.Casper contributes to the study by integrating a transparent characteristic in adult zebrafish thatallows for simpler transparent visualization and observation. We develop casper transgenicprogenies through cross breeding with the transgenic strain of myl7:RFP;annexin-5:YFP .Confocal and fluorescent microscopy used to get accurate, precise imaging and to determinefluorescent protein being activated. 1.1: Generation of homozygous casper / myl7:RFP;annexin-5:YFP zebrafish (Generation F01-F05). 1.2: Screening and sorting the transgenic progeny andIn vivo imaging to validate cardiac morphology through in-vivo confocal imaging. Generation ofhomozygous casper / myl7:RFP;annexin-5:YFP zebrafish: Casper-Annexin5 homozygous stain:Cross breed casper and myl7/Annexin5 fish; F01: Generate the eggs from breeder and grow theembryo to attenuate larvae to screen for transgenic expression. F01 generation, larvae showtransgenic expression (47%). F02: transgenic expression larvae (39%). F02 heterozygous shownormal skin pattern; F03, larval show transgenic expression (43%). F04, transgenic larvae(90%).F04; 100% fishes are phenotypically casper; F05: heterozygous transgenic progeny togrow and continue to generate until achieve 100% homozygous casper-myl7-Annexin5 strain.These novel results provide in-vivo whole organism-based platform to design high throughputscreening and establish new horizon for drug discovery in the Cardiac Disease and Cardio-oncology.

scholarly journals Focal adhesions are essential to drive zebrafish heart valve morphogenesis

2019 ◽  
Vol 218 (3) ◽  
pp. 1039-1054 ◽  
Author(s):  
Felix Gunawan ◽  
Alessandra Gentile ◽  
Ryuichi Fukuda ◽  
Ayele Taddese Tsedeke ◽  
Vanesa Jiménez-Amilburu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Heart Valves ◽  
Focal Adhesions ◽  
Complex Structures ◽  
Loss Of Function ◽  
Integrin Α5β1 ◽  
Vertebrate Model ◽  
Retrograde Blood Flow ◽  
Zebrafish Heart

Elucidating the morphogenetic events that shape vertebrate heart valves, complex structures that prevent retrograde blood flow, is critical to understanding valvular development and aberrations. Here, we used the zebrafish atrioventricular (AV) valve to investigate these events in real time and at single-cell resolution. We report the initial events of collective migration of AV endocardial cells (ECs) into the extracellular matrix (ECM), and their subsequent rearrangements to form the leaflets. We functionally characterize integrin-based focal adhesions (FAs), critical mediators of cell–ECM interactions, during valve morphogenesis. Using transgenes to block FA signaling specifically in AV ECs as well as loss-of-function approaches, we show that FA signaling mediated by Integrin α5β1 and Talin1 promotes AV EC migration and overall shaping of the valve leaflets. Altogether, our investigation reveals the critical processes driving cardiac valve morphogenesis in vivo and establishes the zebrafish AV valve as a vertebrate model to study FA-regulated tissue morphogenesis.

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