scholarly journals Multi-MHz laser-scanning single-cell fluorescence microscopy by spatiotemporally encoded virtual source array

2017 ◽  
Vol 8 (9) ◽  
pp. 4160 ◽  
Author(s):  
Jianglai Wu ◽  
Anson H. L. Tang ◽  
Aaron T. Y. Mok ◽  
Wenwei Yan ◽  
Godfrey C. F. Chan ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Cell ◽  
Laser Scanning ◽  
Virtual Source ◽  
Source Array

scholarly journals Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 861
Author(s):  
Jacopo Cardellini ◽  
Arianna Balestri ◽  
Costanza Montis ◽  
Debora Berti
Keyword(s):  
Drug Delivery ◽  
Fluorescence Microscopy ◽  
Drug Delivery Systems ◽  
Laser Scanning ◽  
Super Resolution ◽  
Laser Scanning Confocal Microscopy ◽  
Delivery Systems ◽  
Scanning Confocal Microscopy ◽  
Biological Phenomena

In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

4D in in vivo 2-photon laser scanning fluorescence microscopy with sample motion in 6 degrees of freedom

2011 ◽  
Vol 200 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Sabine Scheibe ◽  
Mario M. Dorostkar ◽  
Christian Seebacher ◽  
Rainer Uhl ◽  
Frank Lison ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Degrees Of Freedom ◽  
Sample Motion ◽  
6 Degrees Of Freedom

Ultrasonically synthesized organic liquid-filled chitosan microcapsules: part 2: characterization using AFM (atomic force microscopy) and combined AFM–confocal laser scanning fluorescence microscopy

Soft Matter ◽  
2018 ◽  
Vol 14 (16) ◽  
pp. 3192-3201 ◽  
Author(s):  
Srinivas Mettu ◽  
Qianyu Ye ◽  
Meifang Zhou ◽  
Raymond Dagastine ◽  
Muthupandian Ashokkumar
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Organic Liquid ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy (AFM) is used to measure the stiffness and Young's modulus of individual microcapsules that have a chitosan cross-linked shell encapsulating tetradecane.

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy

Luminescence ◽  
2019 ◽  
Vol 34 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Elena Kirilova ◽  
Ilona Mickevica ◽  
Ligita Mezaraupe ◽  
Aleksandrs Puckins ◽  
Ilze Rubenina ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Confocal Laser Scanning ◽  
Confocal Laser

Confocal Laser Scanning Fluorescence Microscopy

2013 ◽  
pp. 362-366
Author(s):  
Alberto Diaspro ◽  
Paolo Bianchini ◽  
Francesca Cella Zanacchi ◽  
Cesare Usai
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Confocal Laser Scanning ◽  
Confocal Laser

How Multiphoton Excitation can Illuminate Biophysics

1997 ◽  
Vol 3 (S2) ◽  
pp. 299-300
Author(s):  
W. W. Webb
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Infrared Light ◽  
Focal Volume ◽  
Multiphoton Excitation ◽  
Fluorescence Excitation ◽  
Photon Excitation ◽  
Rate Of Absorption ◽  
Molecular Excitation

Multiphoton molecular excitation by the strongly focused femtosecond pulses of infrared light generated as an 80 MHZ pulse train by a mode locked laser provides intrinsic submicron three dimensional spatial resolution of fluorescence excitation and photochemistry for laser scanning fluorescence microscopy. Because two-photon excitation requires simultaneous (∼10-16 seconds), absorption of two-photons focused laser intensities of about 1022 photons/cm2s are required. Since the rate of absorption is proportional to the square of the intensity, excitation is limited to the focal volume and is negligible elsewhere along the double cone of the focused illumination. Therefore, out of focus photodamage and fluorescence are generally negligible and laser scanning fluorescence microscopy with multiphoton excitation is intrinsically three dimensionally resolved with no out of focus background. Since the appropriate wave lengths are infrared for multiphoton excitation of ultraviolet or visible absorbing molecules, out of focus photodamage is eliminated. This allows imaging of useful ultraviolet absorbing indicators, vital DNA stains and autofluorescence in living cells with minimal, but not necessarily negligible, photodamage.

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