scholarly journals In vivo micro-vascular imaging and flow cytometry in zebrafish using two-photon excited endogenous fluorescence

2014 ◽  
Vol 5 (3) ◽  
pp. 653 ◽  
Author(s):  
Yan Zeng ◽  
Bo Yan ◽  
Qiqi Sun ◽  
Sicong He ◽  
Jun Jiang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Vascular Imaging ◽  
Two Photon ◽  
Endogenous Fluorescence

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

Two-photon, two-color in vivo flow cytometry to noninvasively monitor multiple circulating cell lines

2007 ◽  
Author(s):  
Eric R. Tkaczyk ◽  
Cheng Frank Zhong ◽  
Jing Yong Ye ◽  
Steve Katnik ◽  
Andrzej Myc ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Lines ◽  
Two Photon ◽  
In Vivo Flow Cytometry

scholarly journals In vivo monitoring of multiple circulating cell populations using two-photon flow cytometry

2008 ◽  
Vol 281 (4) ◽  
pp. 888-894 ◽  
Author(s):  
Eric R. Tkaczyk ◽  
Cheng Frank Zhong ◽  
Jing Yong Ye ◽  
Andrzej Myc ◽  
Thommey Thomas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Populations ◽  
In Vivo Monitoring ◽  
Two Photon

scholarly journals Two-photon in vivo flow cytometry using a fiber probe

2009 ◽  
Author(s):  
Yu-Chung Chang ◽  
Jing Yong Ye ◽  
Thommey P. Thomas ◽  
Zhengyi Cao ◽  
Alina Kotlyar ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fiber Probe ◽  
Two Photon ◽  
In Vivo Flow Cytometry

NIR-emitting semiconducting polymer nanoparticles for in vivo two-photon vascular imaging

2020 ◽  
Vol 8 (9) ◽  
pp. 2666-2672
Author(s):  
Xiao-Ting Gong ◽  
Wenguang Xie ◽  
Jing-Jing Cao ◽  
Shengxiang Zhang ◽  
Kanyi Pu ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Vascular Diseases ◽  
Polymer Nanoparticles ◽  
Semiconducting Polymers ◽  
Vascular Imaging ◽  
Semiconducting Polymer ◽  
Two Photon ◽  
Whole Process ◽  
Versatile Tool

A NIR-emitting TPF probe (NESPN) was prepared using semiconducting polymers and used for continuously monitoring the whole process of ischemic stroke and subsequent reperfusion. This work provides a new and versatile tool for vascular research and diagnosis of vascular diseases.

Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

2012 ◽  
Vol 37 (13) ◽  
pp. 2490 ◽  
Author(s):  
Yan Zeng ◽  
Jin Xu ◽  
Dong Li ◽  
Li Li ◽  
Zilong Wen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Autofluorescence Imaging ◽  
Label Free ◽  
Two Photon ◽  
In Vivo Flow Cytometry

scholarly journals Compartmentalization of algal bioluminescence: autofluorescence of bioluminescent particles in the dinoflagellate Gonyaulax as studied with image-intensified video microscopy and flow cytometry.

1985 ◽  
Vol 100 (5) ◽  
pp. 1435-1446 ◽  
Author(s):  
C H Johnson ◽  
S Inoué ◽  
A Flint ◽  
J W Hastings
Keyword(s):  
Flow Cytometry ◽  
Environmental Conditions ◽  
Video Microscopy ◽  
Emission Characteristics ◽  
Fluorescence Excitation ◽  
Daily Rhythmicity ◽  
Endogenous Fluorescence ◽  
Stimulation Of

Compartmentalization of specialized functions to discrete locales is a fundamental theme of eucaryotic organization in cells. We report here that bioluminescence of the dinoflagellate alga Gonyaulax originates in vivo from discrete subcellular loci that are intrinsically fluorescent. We demonstrate this localization by comparing the loci of fluorescence and bioluminescence as visualized by image-intensified video microscopy. These fluorescent particles appeared to be the same as the previously described in vitro "scintillons." We attribute the endogenous fluorescence to that of the bioluminescence substrate, luciferin, because (a) the fluorescence excitation and emission characteristics are comparable, (b) the autofluorescence is lost after exhaustive stimulation of bioluminescence, and (c) the fluorescence of discharged particles in vitro can be restored by adding luciferin. The fluorescence in vivo exhibits a standard property of circadian (daily) rhythmicity: under constant environmental conditions, the intensity of the particle fluorescence fluctuates cyclically (it is maximal during the night phase and is low during the day). Thus, luciferin is localized within the cell at discrete loci from which the bioluminescence emanates; the cellular quantity of luciferin is rhythmically modulated by the circadian clock.

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