scholarly journals Refractive-index matching enhanced polarization sensitive optical coherence tomography quantification in human brain tissue

2021 ◽  
Author(s):  
Chao Liu ◽  
William Ammon ◽  
Robert Jones ◽  
Jackon Nolan ◽  
Ruopeng Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Refractive Index ◽  
Human Brain ◽  
Brain Tissue ◽  
Optical Coherence ◽  
Human Brain Tissue ◽  
Index Matching ◽  
Refractive Index Matching

scholarly journals Refractive-index matching enhanced polarization-sensitive optical coherence tomography quantification in human brain tissue

2021 ◽  
Author(s):  
Chao J. Liu ◽  
William Ammon ◽  
Robert Jones ◽  
Jackson Nolan ◽  
Ruopeng Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Refractive Index ◽  
White Matter ◽  
Human Brain ◽  
Brain Tissue ◽  
Gray Matter ◽  
Optical Coherence ◽  
Human Brain Tissue ◽  
Index Matching ◽  
Refractive Index Matching

The importance of polarization-sensitive optical coherence tomography (PS-OCT) has been increasingly recognized in human brain imaging. Despite the recent progress of PS-OCT in revealing white matter architecture and orientation, quantification of fine-scale fiber tracts in the human brain cortex has been a challenging problem, due to a low birefringence in the gray matter. In this study, we investigated the effect of refractive index matching by 2,2'-thiodiethanol (TDE) immersion on the improvement of PS-OCT measurements in ex vivo human brain tissue. We obtain the cortical fiber orientation maps in the gray matter, which reveals the radial fibers in the gyrus, the U-fibers along the sulcus, as well as distinct layers of fiber axes exhibiting laminar organization. Further analysis shows that index matching reduces the noise in axis orientation measurements by 56% and 39%, in white and gray matter, respectively. Index matching also enables precise measurements of apparent birefringence, which was underestimated in the white matter by 82% but overestimated in the gray matter by 16% prior to TDE immersion. Mathematical simulations show that the improvements are primarily attributed to the reduction in the tissue scattering coefficient, leading to an enhanced signal-to-noise ratio in deeper tissue regions, which could not be achieved by conventional noise reduction methods.

Imaging ex vivo healthy and pathological human brain tissue with ultra-high-resolution optical coherence tomography

2005 ◽  
Vol 10 (1) ◽  
pp. 011006 ◽  
Author(s):  
Kostadinka Bizheva ◽  
Angelika Unterhuber ◽  
Boris Hermann ◽  
Boris Považay ◽  
Harald Sattmann ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Human Brain ◽  
Brain Tissue ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Human Brain Tissue

scholarly journals Quantitative nontumorous and tumorous human brain tissue assessment using microstructural co- and cross-polarized optical coherence tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Konstantin S. Yashin ◽  
Elena B. Kiseleva ◽  
Alexander A. Moiseev ◽  
Sergey S. Kuznetsov ◽  
Lidia B. Timofeeva ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Human Brain ◽  
Brain Tissue ◽  
Optical Coherence ◽  
Human Brain Tissue

scholarly journals Refractive index measurement of acute rat brain tissue slices using optical coherence tomography

2012 ◽  
Vol 20 (2) ◽  
pp. 1084 ◽  
Author(s):  
Jingjing Sun ◽  
Sung Jin Lee ◽  
Lei Wu ◽  
Malisa Sarntinoranont ◽  
Huikai Xie
Keyword(s):  
Optical Coherence Tomography ◽  
Refractive Index ◽  
Rat Brain ◽  
Brain Tissue ◽  
Optical Coherence ◽  
Tissue Slices ◽  
Refractive Index Measurement ◽  
Index Measurement ◽  
Rat Brain Tissue

scholarly journals RBIO-06. IMPLEMENTATION OF HUMAN INDUCED PLURIPOTENT STEM CELL DERIVED CEREBRAL ORGANOIDS TO MODEL NORMAL TISSUE RADIORESPONSE

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii193-ii193
Author(s):  
Lawrence Bronk ◽  
Sanjay Singh ◽  
Riya Thomas ◽  
Luke Parkitny ◽  
Mirjana Maletic-Savatic ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Tissue ◽  
Model Systems ◽  
Human Brain Tissue ◽  
Post Irradiation ◽  
Mature Neurons ◽  
Induced Pluripotent ◽  
Effects Of Radiation

Abstract Treatment-related sequelae following cranial irradiation have life changing impacts for patients and their caregivers. Characterization of the basic response of human brain tissue to irradiation has been difficult due to a lack of preclinical models. The direct study of human brain tissue in vitro is becoming possible due to advances in stem cell biology, neuroscience, and tissue engineering with the development of organoids as novel model systems which enable experimentation with human tissue models. We sought to establish a cerebral organoid (CO) model to study the radioresponse of normal human brain tissue. COs were grown using human induced pluripotent stem cells and a modified Lancaster protocol. Compositional analysis during development of the COs showed expected populations of neurons and glia. We confirmed a population of microglia-like cells within the model positive for the makers Iba1 and CD68. After 2-months of maturation, COs were irradiated to 0, 10, and 20 Gy using a Shepard Mark-II Cs-137 irradiator and returned to culture. Subsets of COs were prepared for immunostaining at 30- and 70-days post-irradiation. To examine the effect of irradiation on the neural stem cell (NSC) population, sections were stained for SOX2 and Ki-67 expression denoting NSCs and proliferation respectively. Slides were imaged and scored using the CellProfiler software package. The percentage of proliferating NSCs 30-days post-irradiation was found to be significantly reduced for irradiated COs (5.7% (P=0.007) and 3.4% (P=0.001) for 10 and 20 Gy respectively) compared to control (12.7%). The reduction in the proliferating NSC population subsequently translated to a reduced population of NeuN-labeled mature neurons 70 days post-irradiation. The loss of proliferating NSCs and subsequent reduction in mature neurons demonstrates the long-term effects of radiation. Our initial results indicate COs will be a valuable model to study the effects of radiation therapy on normal and diseased human tissue.

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