scholarly journals Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy

2021 ◽  
Vol 12 (10) ◽  
pp. 6205
Author(s):  
Matteo Bernardello ◽  
Radoslaw J. Gora ◽  
Patrick Van Hage ◽  
Gustavo Castro-Olvera ◽  
Emilio J. Gualda ◽  
...  
Keyword(s):  
Protein Dynamics ◽  
Single Molecule ◽  
Light Sheet ◽  
Zebrafish Embryos ◽  
Single Molecule Microscopy ◽  
Intracellular Protein

scholarly journals Single-Molecule Light-Sheet Microscopy with Local Nanopipette Delivery

2021 ◽  
Vol 93 (8) ◽  
pp. 4092-4099
Author(s):  
Bing Li ◽  
Aleks Ponjavic ◽  
Wei-Hsin Chen ◽  
Lee Hopkins ◽  
Craig Hughes ◽  
...  
Keyword(s):  
Single Molecule ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Combinefluent: An Open Source, Low-Cost Laser System for Single-Molecule Microscopy

2021 ◽  
Vol 120 (3) ◽  
pp. 184a
Author(s):  
Dylan George ◽  
Ashley Cadby ◽  
Timothy D. Craggs
Keyword(s):  
Open Source ◽  
Single Molecule ◽  
Low Cost ◽  
Laser System ◽  
Single Molecule Microscopy

scholarly journals EXPRESS: Single-Molecule Fluorescence Techniques for Membrane Protein Dynamics Analysis

2021 ◽  
pp. 000370282110099
Author(s):  
Ziyu Yang ◽  
Haiqi Xu ◽  
Jiayu Wang ◽  
Wei Chen ◽  
Meiping Zhao
Keyword(s):  
Membrane Protein ◽  
Protein Dynamics ◽  
Single Molecule ◽  
Conformational Dynamics ◽  
Correlation Spectroscopy ◽  
Membrane Receptors ◽  
Biological Macromolecules ◽  
Dynamics Analysis ◽  
Single Molecule Fluorescence ◽  
Membrane Protein Dynamics

Fluorescence-based single molecule techniques, mainly including fluorescence correlation spectroscopy (FCS) and single-molecule fluorescence resonance energy transfer (smFRET), are able to analyze the conformational dynamics and diversity of biological macromolecules. They have been applied to analysis of the dynamics of membrane proteins, such as membrane receptors and membrane transport proteins, due to their superior ability in resolving spatio-temporal heterogeneity and the demand of trace amounts of analytes. In this review, we first introduced the basic principle involved in FCS and smFRET. Then we summarized the labelling and immobilization strategies of membrane protein molecules, the confocal-based and TIRF-based instrumental configuration, and the data processing methods. The applications to membrane protein dynamics analysis are described in detail with the focus on how to select suitable fluorophores, labelling sites, experimental setup and analysis methods. In the last part, the remaining challenges to be addressed and further development in this field are also briefly discussed.

Oblique-Sectional Single-Molecule Microscopy

2018 ◽  
Author(s):  
Jeongmin Kim ◽  
Michal Wojcik ◽  
Yuan Wang ◽  
Ke Xu ◽  
Xiang Zhang
Keyword(s):  
Single Molecule ◽  
Single Molecule Microscopy

scholarly journals Cortical actin nodes: Their dynamics and recruitment of podosomal proteins as revealed by super-resolution and single-molecule microscopy

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0188778 ◽  
Author(s):  
Yuki M. Shirai ◽  
Taka A. Tsunoyama ◽  
Nao Hiramoto-Yamaki ◽  
Koichiro M. Hirosawa ◽  
Akihiro C. E. Shibata ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Single Molecule Microscopy ◽  
Cortical Actin

Toward Single-Molecule Microscopy on a Smart Phone

ACS Nano ◽  
2013 ◽  
Vol 7 (10) ◽  
pp. 8340-8343 ◽  
Author(s):  
Saumyakanti Khatua ◽  
Michel Orrit
Keyword(s):  
Single Molecule ◽  
Smart Phone ◽  
Single Molecule Microscopy

Single molecule microscopy methods for the study of DNA origami structures

2010 ◽  
Vol 74 (7) ◽  
pp. 688-698 ◽  
Author(s):  
Victoria Birkedal ◽  
Mingdong Dong ◽  
Monika M. Golas ◽  
Bjoern Sander ◽  
Ebbe Sloth Andersen ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Origami ◽  
Single Molecule Microscopy
Sign in / Sign up

Export Citation Format

Share Document