scholarly journals Label-free identification of human coronary atherosclerotic plaque based on a three-dimensional quantitative assessment of multiphoton microscopy images

2021 ◽  
Vol 12 (5) ◽  
pp. 2979
Author(s):  
Rongli Zhang ◽  
Zhongbiao Xu ◽  
Junhai Hao ◽  
Jia Yu ◽  
Zhiyi Liu ◽  
...  
Keyword(s):  
Atherosclerotic Plaque ◽  
Quantitative Assessment ◽  
Multiphoton Microscopy ◽  
Three Dimensional ◽  
Label Free ◽  
Coronary Atherosclerotic Plaque ◽  
Microscopy Images

scholarly journals Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall

2014 ◽  
Vol 26 (5) ◽  
pp. 685-691
Author(s):  
Michal Sikora ◽  
David Scheiner ◽  
Cornelia Betschart ◽  
Daniele Perucchini ◽  
José María Mateos ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Multiphoton Microscopy ◽  
Three Dimensional ◽  
Vaginal Wall ◽  
Anterior Vaginal Wall ◽  
Label Free

Quantitative Assessment of Coronary Atherosclerotic Plaque Profile by Morphometric Analysis of Angiographic Images

Angiology ◽  
1996 ◽  
Vol 47 (11) ◽  
pp. 1053-1059
Author(s):  
Cataldo Palmieri ◽  
Marco Paterni Rosa Sicari ◽  
Eugenio Picano ◽  
Andrea Biagini ◽  
Mario Marzilli
Keyword(s):  
Atherosclerotic Plaque ◽  
Morphometric Analysis ◽  
Quantitative Assessment ◽  
Coronary Atherosclerotic Plaque

scholarly journals Three dimensional cross-modal image inference: label-free methods for subcellular structure prediction

2017 ◽  
Author(s):  
Chek Ounkomol ◽  
Daniel A. Fernandes ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
...  
Keyword(s):  
Structure Prediction ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Dyes ◽  
Three Dimensional ◽  
Live Cells ◽  
Label Free ◽  
State Changes ◽  
Microscopy Images

AbstractFluorescence microscopy has enabled imaging of key subcellular structures in living cells; however, the use of fluorescent dyes and proteins is often expensive, time-consuming, and damaging to cells. Here, we present a tool for the prediction of fluorescently labeled structures in live cells solely from 3D brightfield microscopy images. We show the utility of this approach in predicting several structures of interest from the same static 3D brightfield image, and show that the same tool can prospectively be used to predict the spatiotemporal position of these structures from a bright-field time series. This approach could also be useful in a variety of application areas, such as cross-modal image registration, quantification of live cell imaging, and determination of cell state changes.

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

scholarly journals Tip dependence of three-dimensional scanning force microscopy images of calcite–water interfaces investigated by simulation and experiments

Nanoscale ◽  
2020 ◽  
Vol 12 (24) ◽  
pp. 12856-12868 ◽  
Author(s):  
Keisuke Miyazawa ◽  
John Tracey ◽  
Bernhard Reischl ◽  
Peter Spijker ◽  
Adam S. Foster ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Scanning Force Microscopy ◽  
Force Microscopy ◽  
Scanning Force ◽  
Microscopy Images

In this study, we have investigated the influence of the tip on the three-dimensional scanning force microscopy (3D-SFM) images of calcite–water interfaces by experiments and simulations.

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