scholarly journals Single-scan HiLo with line-illumination strategy for optical section imaging of thick tissues

2021 ◽  
Vol 12 (4) ◽  
pp. 2373
Author(s):  
Wei Qiao ◽  
Rui Jin ◽  
Tianpeng Luo ◽  
Yafeng Li ◽  
Guoqing Fan ◽  
...  
Keyword(s):  
Optical Section

Surface Characters of Dividing Cells

1958 ◽  
Vol 35 (2) ◽  
pp. 396-399
Author(s):  
SHOZO ISHIZAKA
Keyword(s):  
Sea Urchin ◽  
Optical Section ◽  
Camera Lucida ◽  
Centre Of Gravity ◽  
Whole Process ◽  
Spindle Axis ◽  
Sea Urchin Egg ◽  
Dividing Cells

1. The surface movements during division have been studied by marking the naked surface of the sea-urchin egg with charcoal particles. 2. The contours of the largest optical section, the positions of the particles thereon and the positions of the astral centres are recorded in a series of camera lucida drawings. 3. The drawings are then superimposed, the centre of gravity and spindle axis being used for reference. 4. It is thereby shown that there are two surface rings which remain in the same positions throughout the whole process of division. 5. It is concluded that these rings indicate regions where stresses remain balanced during division.

scholarly journals An Optical Section-Assisted In Vivo Rabbit Model for Capsular Bend and Posterior Capsule Opacification Investigation

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0148553 ◽  
Author(s):  
Pingjun Chang ◽  
Lei Lin ◽  
Qian Zheng ◽  
Fang Yu ◽  
Xiaoyu Yu ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Posterior Capsule Opacification ◽  
Posterior Capsule ◽  
Optical Section

scholarly journals Pollen morphology of Nothofagus (Nothofagaceae, Fagales) and its phylogenetic significance

2016 ◽  
Vol 56 (2) ◽  
pp. 223-245 ◽  
Author(s):  
Damián Andrés Fernández ◽  
Patricio Emmanuel Santamarina ◽  
María Cristina Tellería ◽  
Luis Palazzesi ◽  
Viviana Dora Barreda
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Polar View ◽  
Flowering Plant ◽  
South American ◽  
Optical Section ◽  
South American Species ◽  
Pollen Type ◽  
Fossil Species ◽  
The Family

Abstract Nothofagaceae (southern beeches) are a relatively small flowering plant family of trees confined to the Southern Hemisphere. The fossil record of the family is abundant and it has been widely used as a test case for the classic hypothesis that Antarctica, Patagonia, Australia and New Zealand were once joined together. Although the phylogenetic relationships in Nothofagus appear to be well supported, the evolution of some pollen morphological traits remains elusive, largely because of the lack of ultrastructural analyses. Here we describe the pollen morphology of all extant South American species of Nothofagus, using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and light microscopy (LM), and reconstruct ancestral character states using a well-supported phylogenetic tree of the family. Our results indicate that the main differences between pollen of subgenera Fuscospora (pollen type fusca a) and Nothofagus (pollen type fusca b) are related to the size of microspines (distinguishable or not in optical section), and the thickening of colpi margins (thickened inwards, or thickened both inwards and outwards). In particular, Nothofagus alessandrii, the only extant South American species of subgenus Fuscospora, presents distinctive pollen features that have not been observed in any other species of the genus (i.e. a large granular infratectum and spongy apertural endexine). Species of subgenus Lophozonia are characterized by having the largest pollen grains, with polygonal outline in polar view, microspines distinguishable in optical section, long and non-thickened colpi, and a thin endexine. The reconstruction of character states for the node corresponding to the common ancestor to genus Nothofagus leads us to conclude that the ancestral form of Nothofagaceae should have had: equatorial diameter < 40 μm, circular outline in polar view, microspines distinguishable in optical section, short colpi thickened inwards, and a thin endexine. These features are fully consistent with those present in Nothofagidites senectus Dettmann & Playford, the oldest fossil species of Nothofagaceae recorded in Campanian-Maastrichtian sediments of Gondwana.

Optical Section Retinal Imaging and Wavefront Sensing in Diabetes

2004 ◽  
Vol 81 (10) ◽  
pp. 778-784 ◽  
Author(s):  
MAHNAZ SHAHIDI ◽  
NORMAN P. BLAIR ◽  
MAREK MORI ◽  
RUTH ZELKHA
Keyword(s):  
Retinal Imaging ◽  
Wavefront Sensing ◽  
Optical Section

scholarly journals Increase in Subcellular GSK-3 Clusters in Insulin- and Adrenaline-treated Differentiated Rat Skeletal Muscle Fibres

2020 ◽  
Author(s):  
Katja Fink ◽  
Mateja Lobe Prebil ◽  
Nina Vardjan ◽  
Jorgen Jensen ◽  
Robert Zorec ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Skeletal Muscles ◽  
Metabolic Regulation ◽  
Glycogen Synthase ◽  
Glycogen Synthase Kinase 3 ◽  
Muscle Fibres ◽  
Optical Section ◽  
Rat Skeletal Muscle ◽  
Average Size ◽  
Skeletal Muscle Fibres

Glycogen synthase kinase 3 (GSK-3) plays an important role in metabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate   GKS-3 phosphorylation. The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisation in skeletal muscles.We characterized subcellular localization of (GSK-3) signal protein in fully differentiated muscle fibre by immunofluorescence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of single central optical section of the muscle.We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average size were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linearly related to their quantity.We conclude that subcellular GSK-3 in isolated skeletal muscle fibres is localized in clusters and clustering increased after stimulation with insulin and/or adrenaline.

Distribution of blood platelets flowing in arterioles

1985 ◽  
Vol 248 (3) ◽  
pp. H318-H323 ◽  
Author(s):  
G. J. Tangelder ◽  
H. C. Teirlinck ◽  
D. W. Slaaf ◽  
R. S. Reneman
Keyword(s):  
Blood Platelets ◽  
Median Plane ◽  
General Distribution ◽  
Optical Section ◽  
Intravital Fluorescence Microscopy ◽  
Branch Points ◽  
Flash Duration ◽  
Cross Sectional ◽  
Video Frames ◽  
Platelet Number

The distribution of blood platelets flowing in arterioles (21-35 microns) of the mesentery of anesthetized rabbits was studied using intravital fluorescence microscopy. Sites were selected without upstream branch points within at least 10 vessel diameters. The distribution was determined by counting in flashed video frames the number of platelets present in each of six equal segments across the vessel. Only platelets were counted that could be localized objectively within a thin optical section around the median plane of the vessel. It could be shown that differences in counting volume between the six segments were negligible. Because of the use of flashed pictures (flash duration less than 0.1 ms; interval 180 ms), the method is independent of differences in velocity over the cross-sectional area of the vessel. In all measurements (15 sites in 13 vessels in 10 animals) the distribution was nonuniform, the wall segments containing the highest platelet numbers. The general distribution as calculated from all measurements (total platelet number 6,571) and expressed in percentages was found to be 23.0, 14.6, 12.5, 12.1, 13.6, and 24.2.

Description of a new species, Actus hagiangensis (Mononchida, Mylonchulidae) from Ha Giang province, Vietnam

Zootaxa ◽  
2020 ◽  
Vol 4861 (1) ◽  
pp. 131-138
Author(s):  
TAM T.T. VU
Keyword(s):  
New Species ◽  
Cavity Length ◽  
Buccal Cavity ◽  
Accessory Piece ◽  
Medium Size ◽  
Optical Section ◽  
Male Specimen ◽  
Anterior Position ◽  
Tooth Apex ◽  
A New Species

Actus hagiangensis sp. nov. is described and illustrated from Vietnam. Females of the new species are characterized by medium body size (L = 1.1–1.4 mm), barrel shaped buccal cavity of medium size (26–31 x 13–15 µm) with dorsal tooth apex located 75–81% of buccal cavity length from base and two longitudinal subventral rows, each with four rasp-like denticles; reproductive system didelphic-amphidelphic, vagina short with distinct par refringens vaginae, sclerotized pieces globular in optical section; tail elongate-conoid, slightly ventrally arcuate, with three caudal glands and prominent spinneret. Male with spicules 46 µm in length, and slender, slightly curved gubernaculum, 12 µm long, without accessory piece. This is the first report of a male specimen of the genus Actus. The new species is close to A. salvadoricus but differs by the larger buccal cavity, more anterior position of the dorsal tooth apex and more rounded vaginal sclerotized pieces. 

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