scholarly journals Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror

2021 ◽  
Vol 12 (4) ◽  
pp. 2186
Author(s):  
Nikita Vladimirov ◽  
Friedrich Preusser ◽  
Jan Wisniewski ◽  
Ziv Yaniv ◽  
Ravi Anand Desai ◽  
...  
Keyword(s):  
Light Sheet ◽  
Deformable Mirror ◽  
Glass Interface

scholarly journals Dual-view light-sheet imaging through tilted glass interface using a deformable mirror

2020 ◽  
Author(s):  
N Vladimirov ◽  
F Preusser ◽  
J Wisniewski ◽  
Z Yaniv ◽  
RA Desai ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Microfluidic Channel ◽  
Microfluidic Devices ◽  
Light Sheet ◽  
Stochastic Gradient Descent ◽  
Deformable Mirror ◽  
Optical Aberrations ◽  
Light Sheet Microscopy ◽  
Gradient Descent Algorithm

AbstractLight-sheet microscopy has become one of the primary tools for imaging live developing organisms because of its high speed, low phototoxicity, and optical sectioning capabilities. Detection from multiple sides (multi-view imaging) additionally allows nearly isotropic resolution via computational merging of the views. However, conventional light-sheet microscopes require that the sample is suspended in a gel to allow optical access from two or more sides. At the same time, the use of microfluidic devices is highly desirable for many experiments, but geometric constrains and strong optical aberrations caused by the coverslip titled relative to objectives make the use of multi-view lightsheet challenging for microfluidics.In this paper we describe the use of adaptive optics (AO) to enable multi-view light-sheet microscopy in such microfluidic setup by correcting optical aberrations introduced by the tilted coverslip. The optimal shape of deformable mirror is computed by an iterative stochastic gradient-descent algorithm that optimizes PSF in two orthogonal planes simultaneously. Simultaneous AO correction in two optical arms is achieved via a knife-edge mirror that splits excitation path and combines the detection path.We characterize the performance of this novel microscope setup and, by dual-view light-sheet imaging of C.elegans inside a microfluidic channel, demonstrate a drastic improvement of image quality due to AO and dual-view reconstruction. Our microscope design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experimental conditions and high-content screening.

scholarly journals Video-rate remote refocusing through continuous oscillation of a membrane deformable mirror

2021 ◽  
Author(s):  
Terry Wright ◽  
Hugh Sparks ◽  
Carl Paterson ◽  
Chris Dunsby
Keyword(s):  
Water Immersion ◽  
Numerical Aperture ◽  
Light Sheet ◽  
Field Of View ◽  
Deformable Mirror ◽  
Objective Lens ◽  
Volumetric Imaging ◽  
High Numerical Aperture ◽  
Water Immersion Objective ◽  
Optimisation Procedure

AbstractThis paper presents the use of a deformable mirror (DM) configured to rapidly refocus a microscope employing a high numerical aperture objective lens. An Alpao DM97-15 membrane DM was used to refocus a 40×/0.80 NA water-immersion objective through a defocus range of −50 to 50 μm at 26.3 sweeps per second. We achieved imaging with a mean Strehl metric of > 0.6 over a field of view in the sample of 200×200 μm2 over a defocus range of 77 μm. We describe an optimisation procedure where the mirror is swept continuously in order to avoid known problems of hysteresis associated with the membrane DM employed. This work demonstrates that a DM-based refocusing system could in the future be used in light-sheet fluorescence microscopes to achieve video-rate volumetric imaging.

Large-aperture deformable mirror correction of tiled-grating wavefront error

2006 ◽  
Vol 133 ◽  
pp. 645-648 ◽  
Author(s):  
B. E. Kruschwitz ◽  
R. Jungquist ◽  
J. Qiao ◽  
S. Abbey ◽  
S. E. Dean ◽  
...  
Keyword(s):  
Deformable Mirror ◽  
Large Aperture ◽  
Wavefront Error

DETERMINATION OF LIGHT SHEET THICKNESS IN PIV

1995 ◽  
Vol 2 (3) ◽  
pp. 259-266 ◽  
Author(s):  
Mahmoud F. Maghrebi ◽  
Kiyosi Kawanisi ◽  
Shoitiro Yokosi
Keyword(s):  
Sheet Thickness ◽  
Light Sheet

INVESTIGATION OF FLOW SEPARATION PROPAGATION ON PROPELLER BLADES BY THE TUFTS AND LIGHT SHEET TECHNIQUES

2009 ◽  
Vol 40 (1) ◽  
pp. 103-115
Author(s):  
V. E. Mosharov ◽  
A. A. Orlov ◽  
S. P. Ostroukhov ◽  
V. N. Radchenko
Keyword(s):  
Flow Separation ◽  
Light Sheet ◽  
Propeller Blades

Study and manufacture of a novel micro-deformable mirror

2003 ◽  
Author(s):  
Hongbin Yu ◽  
Haiqing Chen ◽  
Zimin Zhu ◽  
Chao Wang ◽  
Dacheng Zhang ◽  
...  
Keyword(s):  
Deformable Mirror

Basic Physics in Color-Coded EOS Metallization Failures (Differentiating between EOS and ESD)

1998 ◽  
Author(s):  
Leo G. Henry ◽  
J.H. Mazur
Keyword(s):  
Failure Analysis ◽  
Light Microscope ◽  
Structural Changes ◽  
Surface Oxide ◽  
Aluminum Surface ◽  
Surrounding Area ◽  
Die Surface ◽  
Basic Physics ◽  
Direct Contrast ◽  
Glass Interface

Abstract The task of differentiating precisely between EOS and ESD failures continues to be a challenging one for Failure Analysis Engineers. Electrical OverStress (EOS) failures on the die surface (burnt/fused metallization) of an IC can be characterized mainly by the discoloration at the site of the failures. This is in direct contrast to the lack of discoloration characteristic of ESD failures, which occur almost exclusively below the die surface (oxide and junction failures). To aid in this distinction, this paper attempts to present the underlying physics behind the discoloration produced in the EOS failures. For the EOS failures, the metal fuses due to the longer pulse widths (sec to msec), while for the ESD failures, the silicon melts because of the shorter pulse widths (< < 500 nsec) and higher energy. After EOS, the aluminum surface becomes dark and rough and the oxide in the surrounding area becomes deformed and distorted, resulting in the discoloration observed in the light microscope. This EOS discoloration could be due to one or more of the following: 1) morphological and structural changes at the metal/glass interface and the glass itself; 2) changes in the thickness and scattering behavior of the glass and metal in the failed areas.

Correcting for Spherical Aberrations in Solid Immersion Microscopy Using a Deformable Mirror

2011 ◽  
Author(s):  
Y. Lu ◽  
E. Ramsay ◽  
C. Stockbridge ◽  
F. H. Koklu ◽  
A. Yurt ◽  
...  
Keyword(s):  
Failure Analysis ◽  
Error Correction ◽  
Deformable Mirror ◽  
Wafer Thickness ◽  
Wavefront Error ◽  
Substrate Wafer ◽  
Thickness Error

Abstract We present a method for correcting spherical aberrations in solid immersion microscopy through the use of a deformable mirror. Aberrations in solid immersion imaging for failure analysis can be induced through off-axis imaging, errors in lens fabrication or mismatch of design and substrate wafer thickness. RMS wavefront error correction of 30% is demonstrated in the case of substrate wafer thickness error.

Sign in / Sign up

Export Citation Format

Share Document