scholarly journals Label-Free Histological Imaging of Tissues Using Brillouin Light Scattering Contrast

2021 ◽  
Author(s):  
Seungmi Ryu ◽  
Nicola Martino ◽  
Sheldon Kwok ◽  
Liane Bernstein ◽  
Seok-Hyun Yun
Keyword(s):  
Light Scattering ◽  
Label Free ◽  
Brillouin Light Scattering

Brillouin light scattering from surface acoustic waves in photonic microwires

2014 ◽  
Author(s):  
Jean-Charles Beugnot ◽  
Sylvie Lebrun ◽  
Gilles Pauliat ◽  
Vincent Laude ◽  
Thibaut Sylvestre
Keyword(s):  
Light Scattering ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Brillouin Light Scattering

scholarly journals Characterization of Recombinant Adeno-Associated Viruses (rAAVs) for Gene Therapy Using Orthogonal Techniques

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 586
Author(s):  
Liam Cole ◽  
Diogo Fernandes ◽  
Maryam T. Hussain ◽  
Michael Kaszuba ◽  
John Stenson ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Viral Vector ◽  
Structural Characteristics ◽  
Genetic Material ◽  
Label Free ◽  
Gene Therapies ◽  
Multiple Challenges

Viruses are increasingly used as vectors for delivery of genetic material for gene therapy and vaccine applications. Recombinant adeno-associated viruses (rAAVs) are a class of viral vector that is being investigated intensively in the development of gene therapies. To develop efficient rAAV therapies produced through controlled and economical manufacturing processes, multiple challenges need to be addressed starting from viral capsid design through identification of optimal process and formulation conditions to comprehensive quality control. Addressing these challenges requires fit-for-purpose analytics for extensive characterization of rAAV samples including measurements of capsid or particle titer, percentage of full rAAV particles, particle size, aggregate formation, thermal stability, genome release, and capsid charge, all of which may impact critical quality attributes of the final product. Importantly, there is a need for rapid analytical solutions not relying on the use of dedicated reagents and costly reference standards. In this study, we evaluate the capabilities of dynamic light scattering, multiangle dynamic light scattering, and SEC–MALS for analyses of rAAV5 samples in a broad range of viral concentrations (titers) at different levels of genome loading, sample heterogeneity, and sample conditions. The study shows that DLS and MADLS® can be used to determine the size of full and empty rAAV5 (27 ± 0.3 and 33 ± 0.4 nm, respectively). A linear range for rAAV5 size and titer determination with MADLS was established to be 4.4 × 1011–8.7 × 1013 cp/mL for the nominally full rAAV5 samples and 3.4 × 1011–7 × 1013 cp/mL for the nominally empty rAAV5 samples with 3–8% and 10–37% CV for the full and empty rAAV5 samples, respectively. The structural stability and viral load release were also inferred from a combination of DLS, SEC–MALS, and DSC. The structural characteristics of the rAAV5 start to change from 40 °C onward, with increasing aggregation observed. With this study, we explored and demonstrated the applicability and value of orthogonal and complementary label-free technologies for enhanced serotype-independent characterization of key properties and stability profiles of rAAV5 samples.

Brillouin Light Scattering from Magnetic Excitations in Superparamagnetic and Ferromagnetic Co–Al–O Granular Films

2018 ◽  
Vol 87 (4) ◽  
pp. 044707 ◽  
Author(s):  
Akira Yoshihara ◽  
Toshiteru Maeda ◽  
Satoshi Kawamura ◽  
Shintaro Nakamura ◽  
Tsutomu Nojima ◽  
...  
Keyword(s):  
Light Scattering ◽  
Brillouin Light Scattering ◽  
Granular Films ◽  
Magnetic Excitations

Brillouin light scattering in likso4

1983 ◽  
Vol 45 (12) ◽  
pp. 1005-1006 ◽  
Author(s):  
M. Drozdowski ◽  
F. Holuj ◽  
M. Czajkowski
Keyword(s):  
Light Scattering ◽  
Brillouin Light Scattering

Phonon Spectra of a-SiN x : H/Si Heterostructures Observed by Brillouin Light Scattering

1993 ◽  
Vol 10 (10) ◽  
pp. 627-629
Author(s):  
Hua Xia ◽  
Wei Zhang ◽  
Zhifeng Li ◽  
Xuexuan Qu ◽  
Kunji Chen ◽  
...  
Keyword(s):  
Light Scattering ◽  
Brillouin Light Scattering ◽  
Phonon Spectra
Sign in / Sign up

Export Citation Format

Share Document