scholarly journals Hyperspectral imaging and spectral unmixing for improving whole-body fluorescence cryo-imaging

2020 ◽  
Vol 12 (1) ◽  
pp. 395
Author(s):  
Dennis Wirth ◽  
Brook Byrd ◽  
Boyu Meng ◽  
Rendall R. Strawbridge ◽  
Kimberley S. Samkoe ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Spectral Unmixing ◽  
Whole Body

scholarly journals Near infrared hyperspectral imaging and spectral unmixing methods for evaluation of fiber distribution in enriched pasta

2021 ◽  
Vol 343 ◽  
pp. 128517
Author(s):  
Amanda Teixeira Badaró ◽  
José Manuel Amigo ◽  
Jose Blasco ◽  
Nuria Aleixos ◽  
Amanda Rios Ferreira ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Near Infrared ◽  
Spectral Unmixing ◽  
Fiber Distribution

scholarly journals Non-invasive synchronous monitoring of neutrophil migration using whole body near-infrared fluorescence-based imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jack Leslie ◽  
Stuart M. Robinson ◽  
Fiona Oakley ◽  
Saimir Luli
Keyword(s):  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Spectral Unmixing ◽  
Whole Body ◽  
Spectral Signature ◽  
Cell Labelling ◽  
Cellular Interactions ◽  
Non Invasive

AbstractAdvances in fluorescence imaging coupled with the generation of near infrared probes have significantly improved the capabilities of non-invasive, real-time imaging in whole animals. In this study we were able to overcome a limitation of in vivo fluorescence imaging and have established a dual cell tracking method where two different cell types can be monitored according to the spectral signature of the cell labelling fluorophore. Using a mouse model of acute liver injury, we have characterised the in vivo migration patterns of wild type and transgenic neutrophils with impaired chemotaxis. Here, we were able to demonstrate that IVIS provides a sensitive multiplexing technology to differentiate two different cell populations based on the spectral signature of the cell labelling fluorophores. This spectral unmixing methodology has the potential to uncover multidimensional cellular interactions involved in many diseases such as fibrosis and cancer. In vivo spectral un-mixing provides a useful tool for monitoring multiple biological process in real-time in the same animal.

scholarly journals Non-Linear Spectral Unmixing for the Estimation of the Distribution of Graphene Oxide Deposition on 3D Printed Composites

2020 ◽  
Vol 10 (21) ◽  
pp. 7792
Author(s):  
Giorgio Licciardi ◽  
Costantino Del Gaudio ◽  
Jocelyn Chanussot
Keyword(s):  
Graphene Oxide ◽  
Hyperspectral Imaging ◽  
Materials Science ◽  
Hyperspectral Image ◽  
Polymer Matrix Composites ◽  
Spectral Unmixing ◽  
Science Field ◽  
Hyperspectral Image Analysis ◽  
Custom Made ◽  
3D Printed

Hyperspectral analysis is a well-established technique that can be suitably implemented in several application fields, including materials science. This approach allows us to deal with data samples containing spatial and spectral information at very high resolution, thus enabling us to evaluate materials properties at a nanoscale level. As a proof of concept, hyperspectral imaging was here considered to investigate 3D printed polymer matrix composites, considering graphene oxide (GO) as a nanofiller. Commercial polycaprolactone and polylactic acid filaments were firstly treated with GO to be then printed into testing specimens. Raman analysis was performed to assess the GO distribution on samples surface by mapping different regions of interest and the collected data were the input of a custom-made algorithm for hyperspectral image analysis, tailored to detect the GO signature. Findings showed a valuable matching to Raman maps and were also characterized by the positive feature of avoiding to set specific conditions to perform the investigation as GO Raman distribution was carried out by fixing the wavenumber at 1580 cm−1, which is representative of the G band of the nanofiller. This occurrence might lead to an uneven intensity representation related to possible peak shifts which can bias the acquired results. Differently, hyperspectral imaging needs a minimal set of data input, i.e., the spectral signatures of neat materials, to directly identify the searched nanomaterial. More in-depth investigations need to be performed to fully validate the proposed approach, but the here presented results already show the potential and versatility of hyperspectral analysis in the materials science field.

scholarly journals Developing a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging

2020 ◽  
Author(s):  
Boyu Meng ◽  
Brook K. Byrd ◽  
Dennis J. Wirth ◽  
Rendall R. Strawbridge ◽  
Scott C. Davis
Keyword(s):  
Fluorescence Imaging ◽  
Hyperspectral Imaging ◽  
Whole Body

scholarly journals Application of Spectroscopic and Hyperspectral Imaging Techniques for Rapid and Nondestructive Investigation of Jewish Ritual Parchment

2020 ◽  
Vol 7 ◽  
Author(s):  
Ioana Maria Cortea ◽  
Luminiţa Ghervase ◽  
Lucian Ratoiu ◽  
Roxana Rădvan
Keyword(s):  
Hyperspectral Imaging ◽  
Multispectral Imaging ◽  
Imaging Techniques ◽  
Inorganic Compounds ◽  
Historical Context ◽  
Short Wave ◽  
Spectral Unmixing ◽  
Materials Used ◽  
Iron Gall ◽  
Jewish Ritual

The article presents a multi-analytic investigation of a severely degraded Jewish ritual parchment coming from a private collection. The main aim of the study was to obtain key information on the parchment manufacturing technique and original materials used, information that could help understand the historical context of the object. To this aim, a series of noninvasive investigations were carried out by means of multi- and hyperspectral imaging, Fourier transform infrared (FTIR) spectroscopy and X-ray fluorescence (XRF) spectroscopy. Specific degradations and mapping of previous conservation treatments could be highlighted via multispectral imaging. Short-wave infrared images indicated the use of both iron gall and carbon black ink, probably one related to the original writing and the other to a later intervention. To improve the imaging of degraded or partially lost text, a linear spectral unmixing classification of the HSI dataset was proposed that showed promising results, allowing it to be applied to similar objects. XRF analysis offered an in-depth view of the chemical fingerprint of the original iron gall ink and critical findings on the existence of other inorganic compounds originating from the parchment manufacture. Registered FTIR data indicated denaturation of the collagen fibers and the presence of fungal-derived calcium oxalates and zinc carboxylates. In accordance with ancient Jewish parchment preparation techniques, the use of calcium sulfate, vegetable tannins, and oils was also inferred from the registered infrared spectra. The corroborated results offer valuable information on the origin, production technology, and overall degradation state of the parchment manuscript. Not least, the findings could be of great interest for conservators and restorers in the field.

Hyperspectral imaging and spectral unmixing of stained tissue sections using a spectrally programmable light engine

2007 ◽  
Author(s):  
N. B. MacKinnon ◽  
M. Khojasteh ◽  
P. M. Lane ◽  
C. E. MacAulay ◽  
M. Guillaud ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Spectral Unmixing ◽  
Tissue Sections ◽  
Light Engine
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