scholarly journals Continuous imaging of large-volume tissues with a machinable optical clearing method at subcellular resolution

2020 ◽  
Vol 11 (12) ◽  
pp. 7132
Author(s):  
Can Zhou ◽  
Ting Zheng ◽  
Ting Luo ◽  
Cheng Yan ◽  
Qingtao Sun ◽  
...  
Keyword(s):  
Large Volume ◽  
Optical Clearing ◽  
Subcellular Resolution ◽  
Clearing Method

The Optical Clearing Method

2019 ◽  
Author(s):  
Luís Manuel Couto Oliveira ◽  
Valery Victorovich Tuchin
Keyword(s):  
Optical Clearing ◽  
Clearing Method

UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging

2018 ◽  
Author(s):  
Guiye Li ◽  
Yingchao Li ◽  
Lina Liu ◽  
Ang Liu ◽  
Shuangchen Ruan ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Optical Clearing ◽  
Clearing Method

DAS: A simple, efficient, scalable and DiI-compatible optical clearing method for intact systems

2018 ◽  
Author(s):  
Lingling Chen ◽  
Lina Liu ◽  
Guiye Li ◽  
Ang Liu ◽  
Yingchao Li ◽  
...  
Keyword(s):  
Optical Clearing ◽  
Clearing Method

scholarly journals UbasM: An effective balanced optical clearing method for intact biomedical imaging

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Lingling Chen ◽  
Guiye Li ◽  
Yamin Li ◽  
Yingchao Li ◽  
Haiou Zhu ◽  
...  
Keyword(s):  
Biomedical Imaging ◽  
Optical Clearing ◽  
Clearing Method

ASSESSMENT OF OPTICAL CLEARING INDUCED IMPROVEMENT OF LASER SPECKLE CONTRAST IMAGING

2010 ◽  
Vol 03 (03) ◽  
pp. 159-167 ◽  
Author(s):  
JING WANG ◽  
DAN ZHU ◽  
MIN CHEN ◽  
XIAOJING LIU
Keyword(s):  
Blood Flow ◽  
Flow Velocity ◽  
Laser Speckle ◽  
Contrast Imaging ◽  
Laser Speckle Contrast Imaging ◽  
Optical Clearing ◽  
Speckle Contrast ◽  
Tissue Phantom ◽  
Contrast Analysis ◽  
Clearing Method

Laser Speckle Contrast Imaging (LSCI) plays an important role in studying blood flow, but suffers from limited penetration depth of light in turbid tissue. The strong scattering of tissue obviously reduces the image contrast which decreases the sensitivity to flow velocity. Some image processing or optical clearing methods have been proposed to lessen the deficiency, but quantitative assessment of improvement is seldom given. In this study, LSCI was applied to monitor the blood flow through a capillary embedded within various tissue phantoms at depths of 0.25, 0.45, 0.65, 0.85 and 1.05 mm, and the flow velocity in capillary was controllable from 0 to 4 mm/s. Here, glycerol, a common optical clearing agent, was mixed with Intralipid at different volume ratio to make the reduced scattering coefficient of tissue phantom decrease from 13.00 to 0.50 cm-1. The quantitative analysis demonstrates that the optical clearing method can obviously enhance the image contrast, imaging depth, and sensitivity to blood flow velocity. Comparing the Laser Speckle Contrast Analysis methods and the optical clearing method, we find that for typical turbid tissue, the sensitivity to velocity estimated by the Laser Speckle Temporal Contrast Analysis (LSTCA) is twice of that by the Laser Speckle Spatial Contrast Analysis (LSSCA); while the sensitivity to velocity estimated by using the two analysis methods has a 10-fold increase, respectively, if addition of glycerol makes the reduced scattering coefficient of tissue phantom decrease by 30%. Combining the LSTCA and the optical clearing method, the sensitivity to flow velocity will be further enhanced.

scholarly journals Fast 3D Clear: A Fast, Aqueous-Based, Reversible Three-Day Tissue Clearing Method for Adult and Embryonic Mouse Brain and Whole Body

2021 ◽  
Author(s):  
Stylianos Kosmidis ◽  
Adrian Negrean ◽  
Alex Dranovsky ◽  
Attila Losonczy ◽  
Eric Kandel
Keyword(s):  
Light Sheet ◽  
Whole Body ◽  
Two Dimensional ◽  
Embryonic Mouse ◽  
Optical Clearing ◽  
Vast Array ◽  
High Transparency ◽  
Mouse Tissues ◽  
Wide Range ◽  
Clearing Method

To date, a variety of optical clearing methods have emerged that serve as powerful tools to study intact whole organ anatomy and neuronal circuits. Here we describe a newly developed, aqueous-based clearing protocol named Fast 3D Clear, that relies on THF for tissue dehydration/rehydration and utilizes Histodenz as clearing solution. Fast 3D Clear requires three days to achieve high transparency of adult and embryonic mouse tissues, while maintaining their anatomical integrity, and preserving a vast array of transgenic and viral/dye fluorophores, such as GCaMP3/6, tdTomato, Fast Blue, and IRF670. A unique advantage of Fast 3D Clear is its complete reversibility, and thus compatibility with tissue sectioning and immunohistochemistry. Fast 3D Clear can be easily and quickly applied to a wide range of biomedical studies, thereby greatly facilitating the acquisition of high-resolution, three- and two-dimensional images with light sheet and confocal microscopy.

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