scholarly journals In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system

2020 ◽  
Vol 11 (10) ◽  
pp. 6027
Author(s):  
Ric John L. Ombid ◽  
Glenn G. Oyong ◽  
Esperanza C. Cabrera ◽  
Wilfred V. Espulgar ◽  
Masato Saito ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Optical Trapping ◽  
Leukemia Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Membrane Elasticity

scholarly journals A Model In Vitro Study Using Hypericin: Tumor-Versus Necrosis-Targeting Property and Possible Mechanisms

Biology ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 13 ◽  
Author(s):  
Yue Li ◽  
Shuncong Wang ◽  
Yuanyu Zhao ◽  
Hexige Saiyin ◽  
Xiaoyan He ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Confocal Microscopy ◽  
Cell Membrane ◽  
Cell Lines ◽  
Fluorescence Intensity ◽  
Subcellular Distribution ◽  
In Vitro Study ◽  
Vitro Study ◽  
Binding Theory

Hypericin (Hyp) had been explored as a tumor-seeking agent for years; however, more recent studies showed its necrosis-avidity rather than cancer-seeking property. To further look into this discrepancy, we conducted an in vitro study on Hyp retention in vital and dead cancerous HepG2 and normal LO2 cell lines by measuring the fluorescence intensity and concentration of Hyp in cells. To question the DNA binding theory for its necrosis-avidity, the subcellular distribution of Hyp was also investigated to explore the possible mechanisms of the necrosis avidity. The fluorescence intensity and concentration are significantly higher in dead cells than those in vital cells, and this difference did not differ between HepG2 and LO2 cell lines. Hyp was taken up in vital cells in the early phase and excreted within hours, whereas it was retained in dead cells for more than two days. Confocal microscopy showed that Hyp selectively accumulated in lysosomes rather than cell membrane or nuclei. Hyp showed a necrosis-avid property rather than cancer-targetability. The long-lasting retention of Hyp in dead cells may be associated with halted energy metabolism and/or binding with certain degraded cellular substrates. Necrosis-avidity of Hyp was confirmed, which may be associated with halted energy metabolism in dead LO2 or HepG2 cells.

Anticancer Activity of Brevinin-2R Peptide and its Two Analogues Against Myelogenous Leukemia Cell Line as Natural Treatments: An In Vitro Study

2019 ◽  
Vol 26 (2) ◽  
pp. 1013-1020
Author(s):  
Robab Hassanvand Jamadi ◽  
Saeed Khalili ◽  
Tooba Mirzapour ◽  
Hashem Yaghoubi ◽  
Zahra Sadat Hashemi ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Cell Line ◽  
Leukemia Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Myelogenous Leukemia ◽  
Leukemia Cell Line
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