scholarly journals High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip

2020 ◽  
Vol 11 (8) ◽  
pp. 4397 ◽  
Author(s):  
Federico Sala ◽  
Michele Castriotta ◽  
Petra Paiè ◽  
Andrea Farina ◽  
Sarah D’Annunzio ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
3D Imaging ◽  
Single Cells ◽  
Light Sheet ◽  
On Chip ◽  
Light Sheet Fluorescence Microscopy

Droplet-based light-sheet fluorescence microscopy for high-throughput sample preparation, 3-D imaging and quantitative analysis on a chip

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2193-2197 ◽  
Author(s):  
Hao Jiang ◽  
Tingting Zhu ◽  
Hao Zhang ◽  
Jun Nie ◽  
Zeyi Guan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Microscopy ◽  
Sample Preparation ◽  
High Throughput ◽  
Light Sheet ◽  
Droplet Microfluidics ◽  
On Chip ◽  
Light Sheet Fluorescence Microscopy

An optofluidic device interfaces droplet microfluidics with light-sheet fluorescence microscopy for on-chip, high-throughput sample compartmentalization and 3-D imaging.

scholarly journals Optofluidic lab-on-chips for high throughput 3D imaging of cells and tissues

2019 ◽  
Vol 215 ◽  
pp. 11002
Author(s):  
Federico Sala ◽  
Petra Paiè ◽  
Roberto Memeo ◽  
Roberto Osellame ◽  
Andrea Farina ◽  
...  
Keyword(s):  
High Throughput ◽  
Chemical Etching ◽  
3D Imaging ◽  
Single Cells ◽  
Three Dimensions ◽  
Single Cell Level ◽  
Cell Level ◽  
Optical Components ◽  
Femtosecond Laser Micromachining ◽  
On Chip

Optofludic lab-on-chips are compact microsystems that allow the manipulation, the analysis and the imaging of cells with high throughput even at the single cell level. The optofluidic microscopes on chip here presented are fabricated by femtosecond laser micromachining followed by chemical etching. The technique is ideally suited for rapid prototyping and allows 3D geometries and perfect alignment between microfluidic and optical components networks. The devices have been validated on cancer cell lines by imaging in three dimensions both clusters of cells growing on spheres and single cells.

scholarly journals Automatic imaging of Drosophila embryos with light sheet fluorescence microscopy on chip

2020 ◽  
Author(s):  
Roberto Memeo ◽  
Petra Paiè ◽  
Federico Sala ◽  
Michele Castriotta ◽  
Chiara Guercio ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Light Sheet ◽  
On Chip ◽  
Light Sheet Fluorescence Microscopy ◽  
Drosophila Embryos

Light sheet fluorescence microscopy for 3D imaging of Ca2+ dynamics in neuronal cultures

2021 ◽  
Author(s):  
Pablo Loza-Alvarez ◽  
Gustavo Castro-Olvera ◽  
Jorge A. Madrid-Wolff ◽  
Omar E. Olarte ◽  
Emilio J. Gualda ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
3D Imaging ◽  
Light Sheet ◽  
Neuronal Cultures ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Automated High-Throughput Light-Sheet Fluorescence Microscopy of Larval Zebrafish

2018 ◽  
Author(s):  
Savannah L. Logan ◽  
Christopher Dudley ◽  
Ryan P. Baker ◽  
Michael J. Taormina ◽  
Edouard A. Hay ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Three Dimensional ◽  
Light Sheet ◽  
Individual Variability ◽  
Three Dimensional Imaging ◽  
Larval Zebrafish ◽  
Neutrophil Number ◽  
Spatially Resolved ◽  
Light Sheet Fluorescence Microscopy

ABSTRACTLight sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation. Here, we describe an automated high-throughput light sheet fluorescence microscope in which specimens are positioned by and imaged within a fluidic system integrated with the sheet excitation and detection optics. We demonstrate the ability of the instrument to rapidly examine live specimens with minimal manual intervention by imaging fluorescent neutrophils over a nearly 0.3 mm3 volume in dozens of larval zebrafish. In addition to revealing considerable inter-individual variability in neutrophil number, known previously from labor-intensive methods, three-dimensional imaging allows assessment of the correlation between the bulk measure of total cellular fluorescence and the spatially resolved measure of actual neutrophil number per animal. We suggest that our simple experimental design should considerably expand the scope and impact of light sheet imaging in the life sciences.

High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

2019 ◽  
Author(s):  
Stylianos E. Psycharakis ◽  
Evangelos Liapis ◽  
Athanasios Zacharopoulos ◽  
Mariam-Eleni Oraiopoulou ◽  
Chrysoula Aivalioti ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
3D Imaging ◽  
Light Sheet ◽  
Tumor Spheroids ◽  
Secondary Tumor ◽  
Light Sheet Fluorescence Microscopy

scholarly journals A Bright Fluorescent Probe for H2S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy

2015 ◽  
Vol 137 (32) ◽  
pp. 10216-10223 ◽  
Author(s):  
Matthew D. Hammers ◽  
Michael J. Taormina ◽  
Matthew M. Cerda ◽  
Leticia A. Montoya ◽  
Daniel T. Seidenkranz ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescent Probe ◽  
3D Imaging ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0198705 ◽  
Author(s):  
Savannah L. Logan ◽  
Christopher Dudley ◽  
Ryan P. Baker ◽  
Michael J. Taormina ◽  
Edouard A. Hay ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Light Sheet ◽  
Larval Zebrafish ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Cytotoxic effects and tolerability of gemcitabine and axitinib in a xenograft model for c-myc amplified medulloblastoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefanie Schwinn ◽  
Zeinab Mokhtari ◽  
Sina Thusek ◽  
Theresa Schneider ◽  
Anna-Leena Sirén ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Tumor Growth ◽  
Intensive Therapy ◽  
Xenograft Model ◽  
Light Sheet ◽  
Tumor Control ◽  
Orthotopic Model ◽  
Group 3 ◽  
Light Sheet Fluorescence Microscopy

AbstractMedulloblastoma is the most common high-grade brain tumor in childhood. Medulloblastomas with c-myc amplification, classified as group 3, are the most aggressive among the four disease subtypes resulting in a 5-year overall survival of just above 50%. Despite current intensive therapy regimens, patients suffering from group 3 medulloblastoma urgently require new therapeutic options. Using a recently established c-myc amplified human medulloblastoma cell line, we performed an in-vitro-drug screen with single and combinatorial drugs that are either already clinically approved or agents in the advanced stage of clinical development. Candidate drugs were identified in vitro and then evaluated in vivo. Tumor growth was closely monitored by BLI. Vessel development was assessed by 3D light-sheet-fluorescence-microscopy. We identified the combination of gemcitabine and axitinib to be highly cytotoxic, requiring only low picomolar concentrations when used in combination. In the orthotopic model, gemcitabine and axitinib showed efficacy in terms of tumor control and survival. In both models, gemcitabine and axitinib were better tolerated than the standard regimen comprising of cisplatin and etoposide phosphate. 3D light-sheet-fluorescence-microscopy of intact tumors revealed thinning and rarefication of tumor vessels, providing one explanation for reduced tumor growth. Thus, the combination of the two drugs gemcitabine and axitinib has favorable effects on preventing tumor progression in an orthotopic group 3 medulloblastoma xenograft model while exhibiting a favorable toxicity profile. The combination merits further exploration as a new approach to treat high-risk group 3 medulloblastoma.

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