scholarly journals Forward multiple scattering dominates speckle decorrelation in whole-blood flowmetry using optical coherence tomography

2020 ◽  
Vol 11 (4) ◽  
pp. 1947 ◽  
Author(s):  
Natalie G. Ferris ◽  
Taylor M. Cannon ◽  
Martin Villiger ◽  
Brett E. Bouma ◽  
Néstor Uribe-Patarroyo
Keyword(s):  
Optical Coherence Tomography ◽  
Multiple Scattering ◽  
Whole Blood ◽  
Optical Coherence ◽  
Blood Flowmetry

scholarly journals Aberration-diverse optical coherence tomography for suppression of multiple scattering and speckle

2018 ◽  
Vol 9 (10) ◽  
pp. 4919 ◽  
Author(s):  
Siyang Liu ◽  
Michael R. E. Lamont ◽  
Jeffrey A. Mulligan ◽  
Steven G. Adie
Keyword(s):  
Optical Coherence Tomography ◽  
Multiple Scattering ◽  
Optical Coherence

scholarly journals Spectroscopic Optical Coherence Tomography by Using Multiple Multipole Expansion

Photonics ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 44
Author(s):  
Hon Seck ◽  
Ying Zhang
Keyword(s):  
Optical Coherence Tomography ◽  
Multiple Scattering ◽  
Frequency Analysis ◽  
Multipole Expansion ◽  
Processing Method ◽  
Optical Coherence ◽  
Time Frequency ◽  
Higher Order Terms ◽  
Scattered Fields ◽  
Lower Order Terms

This paper presents a pre-processing method to remove multiple scattering artifacts in spectroscopic optical coherence tomography (SOCT) using time–frequency analysis approaches. The method uses a multiple multipole expansion approach to model the light fields in SOCT. It is shown that the multiple scattered fields can be characterized by higher order terms of the multiple multipole expansion. Hence, the multiple scattering artifact can thus be eliminated by applying the time–frequency transform on the SOCT measurements characterized by the lower order terms. Simulation and experimental results are presented to show the effectiveness of the proposed pre-processing method.

scholarly journals Quantification of total haemoglobin concentrations in human whole blood by spectroscopic visible-light optical coherence tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Colin Veenstra ◽  
Saskia Kruitwagen ◽  
Dafne Groener ◽  
Wilma Petersen ◽  
Wiendelt Steenbergen ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Visible Light ◽  
Correlation Coefficient ◽  
Whole Blood ◽  
Ex Vivo ◽  
Pearson Correlation ◽  
Pearson Correlation Coefficient ◽  
Optical Coherence ◽  
Human Whole Blood ◽  
Total Haemoglobin

Abstract The non-invasive quantification of total haemoglobin concentrations [tHb] is highly desired for the assessment of haematologic disorders in vulnerable patient groups, but invasive blood sampling is still the gold standard in current clinical practice. This work demonstrates the potential of visible-light spectroscopic optical coherence tomography (sOCT) for quantifying the [tHb] in human whole blood. To accurately quantify the [tHb] from the substantial optical attenuation by blood in the visible wavelength range, we used a combination of zero-delay acquisition and focus tracking that ensures optimal system sensitivity at any depth inside the sample. Subsequently, we developed an analysis model to adequately correct for the high scattering contribution by red blood cells to the sOCT signal. We validate our method and compare it to conventional sOCT (without focus tracking and zero-delay acquisition) through ex-vivo measurements on flowing human whole blood, with [tHb] values in the clinical range of 7–23 g/dL. For our method with optimized sensitivity, the measured and expected values correlate well (Pearson correlation coefficient = 0.89, p < 0.01), with a precision of 3.8 g/dL. This is a considerable improvement compared to conventional sOCT (Pearson correlation coefficient = 0.59, p = 0.16; precision of 9.1 g/dL).

scholarly journals Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carlos Cuartas-Vélez ◽  
Colin Veenstra ◽  
Saskia Kruitwagen ◽  
Wilma Petersen ◽  
Nienke Bosschaart
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Density ◽  
Whole Blood ◽  
Ex Vivo ◽  
Single Step ◽  
Optical Coherence ◽  
Non Invasive ◽  
Fitting Procedure ◽  
Total Haemoglobin ◽  
Numerical Optimisation

AbstractSpectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9.10 g/dL with a bias of 1.50 g/dL. This precision improved by acquiring data with a combination of focus tracking and zero-delay acquisition (FZA) that compensated for experimental limitations, increasing to 3.80 g/dL with a bias of 1.50 g/dL. Nevertheless, sOCT precision should improve at least to $$\sim 2$$ ∼ 2  g/dL to be clinically relevant. Therefore, sOCT-based [tHb] determinations require the development of new analysis methods that reduce the variability of [tHb] estimations. In this work, we aim to increase sOCT precision by retrieving the [tHb] content from a numerical optimisation of the optical density (OD), while considering the blood absorption flattening effect. The OD-based approach simplifies previous two-step Lambert–Beer fitting approaches to a single step, thereby reducing errors during the fitting procedure. We validated our model with ex-vivo [tHb] measurements on flowing whole-blood samples in the clinical range (7–23 g/dL). Our results show that, with the new model, conventional sOCT can determine [tHb] with a precision of 3.09 g/dL and a bias of 0.86 g/dL compared to a commercial blood analyser. We present further precision improvement by combining the OD methodology with FZA, leading to a precision of 2.08 g/dL with a bias of 0.46 g/dL.

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