scholarly journals Three-dimensional imaging of cell and extracellular matrix elasticity using quantitative micro-elastography

2020 ◽  
Vol 11 (2) ◽  
pp. 867 ◽  
Author(s):  
Matt S. Hepburn ◽  
Philip Wijesinghe ◽  
Luke G. Major ◽  
Jiayue Li ◽  
Alireza Mowla ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Matrix Elasticity

scholarly journals Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Kohei Togami ◽  
Hiroaki Ozaki ◽  
Yuki Yumita ◽  
Anri Kitayama ◽  
Hitoshi Tada ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Pulmonary Fibrosis ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Collagen I ◽  
Immunofluorescence Staining ◽  
Therapeutic Effects ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Tomato Lectin

Idiopathic pulmonary fibrosis is a progressive, chronic lung disease characterized by the accumulation of extracellular matrix proteins, including collagen and elastin. Imaging of extracellular matrix in fibrotic lungs is important for evaluating its pathological condition as well as the distribution of drugs to pulmonary focus sites and their therapeutic effects. In this study, we compared techniques of staining the extracellular matrix with optical tissue-clearing treatment for developing three-dimensional imaging methods for focus sites in pulmonary fibrosis. Mouse models of pulmonary fibrosis were prepared via the intrapulmonary administration of bleomycin. Fluorescent-labeled tomato lectin, collagen I antibody, and Col-F, which is a fluorescent probe for collagen and elastin, were used to compare the imaging of fibrotic foci in intact fibrotic lungs. These lung samples were cleared using the ClearT2 tissue-clearing technique. The cleared lungs were two dimensionally observed using laser-scanning confocal microscopy, and the images were compared with those of the lung tissue sections. Moreover, three-dimensional images were reconstructed from serial two-dimensional images. Fluorescent-labeled tomato lectin did not enable the visualization of fibrotic foci in cleared fibrotic lungs. Although collagen I in fibrotic lungs could be visualized via immunofluorescence staining, collagen I was clearly visible only until 40 μm from the lung surface. Col-F staining facilitated the visualization of collagen and elastin to a depth of 120 μm in cleared lung tissues. Furthermore, we visualized the three-dimensional extracellular matrix in cleared fibrotic lungs using Col-F, and the images provided better visualization than immunofluorescence staining. These results suggest that ClearT2 tissue-clearing treatment combined with Col-F staining represents a simple and rapid technique for imaging fibrotic foci in intact fibrotic lungs. This study provides important information for imaging various organs with extracellular matrix-related diseases.

The Role of Three-Dimensional Imaging in Evaluation of the Sinonasal Mass

1996 ◽  
Vol 34 (1) ◽  
pp. 27
Author(s):  
Sue Yon Shim ◽  
Ki Joon Sung ◽  
Young Ju Kim ◽  
In Soo Hong ◽  
Myung Soon Kim ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Sinonasal Mass

Synchrotron and neutron laminography for three-dimensional imaging of devices and flat material specimens

2012 ◽  
Vol 103 (2) ◽  
pp. 170-173 ◽  
Author(s):  
Lukas Helfen ◽  
Thilo F. Morgeneyer ◽  
Feng Xu ◽  
Mark N. Mavrogordato ◽  
Ian Sinclair ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging

Three-Dimensional Imaging through Shock-Waves at Ultra-High Speed.

2018 ◽  
Author(s):  
Yi Chen Mazumdar ◽  
Michael E. Smyser ◽  
Jeffery Dean Heyborne ◽  
Daniel Robert Guildenbecher
Keyword(s):  
Shock Waves ◽  
High Speed ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Ultra High Speed

Induction of in Vivo Ectopic Hematopoiesis by a Three-Dimensional Structured Extracellular Matrix Derived from Decellularized Cancellous Bone

2019 ◽  
Vol 5 (11) ◽  
pp. 5669-5680 ◽  
Author(s):  
Naoko Nakamura ◽  
Tsuyoshi Kimura ◽  
Kwangwoo Nam ◽  
Toshiya Fujisato ◽  
Hiroo Iwata ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cancellous Bone ◽  
Three Dimensional
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