Graded-Field White-Light Microscopy

2006 ◽  
Author(s):  
Ran Yi ◽  
Kengyeh Chu ◽  
Jerome Mertz
Keyword(s):  
Light Microscopy ◽  
White Light

A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting

2014 ◽  
Vol 21 (1) ◽  
pp. 239-248 ◽  
Author(s):  
Ambroise Marin ◽  
Emmanuel Denimal ◽  
Stéphane Guyot ◽  
Ludovic Journaux ◽  
Paul Molin
Keyword(s):  
Image Processing ◽  
Fourier Transform ◽  
Light Microscopy ◽  
White Light ◽  
Hough Transform ◽  
High Variability ◽  
Cell Counting ◽  
Cell Segmentation ◽  
Primary Measurement

AbstractIn biology, cell counting is a primary measurement and it is usually performed manually using hemocytometers such as Malassez blades. This work is tedious and can be automated using image processing. An algorithm based on Fourier transform filtering and the Hough transform was developed for Malassez blade grid extraction. This facilitates cell segmentation and counting within the grid. For the present work, a set of 137 images with high variability was processed. Grids were accurately detected in 98% of these images.

Impact of non-equilibrium molecular packings on singlet fission in microcrystals observed using 2D white-light microscopy

2019 ◽  
Vol 12 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Andrew C. Jones ◽  
Nicholas M. Kearns ◽  
Jia-Jung Ho ◽  
Jessica T. Flach ◽  
Martin T. Zanni
Keyword(s):  
Light Microscopy ◽  
White Light ◽  
Singlet Fission ◽  
Non Equilibrium

Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography

2016 ◽  
Vol 55 (15) ◽  
pp. 4261 ◽  
Author(s):  
M. Blattmann ◽  
S. Kretschmer ◽  
S. Thiele ◽  
C. Ataman ◽  
H. Zappe ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Light Microscopy ◽  
White Light ◽  
Optical Coherence

Design, assembly and calibration of white-light microscopy interferometer

2015 ◽  
Author(s):  
Shuai Wang ◽  
Zhishan Gao ◽  
Minjue Li ◽  
Jingfei Ye ◽  
Jinlong Cheng ◽  
...  
Keyword(s):  
Light Microscopy ◽  
White Light

scholarly journals Survival after resection of brain metastases with white light microscopy versus fluorescence-guidance: A matched cohort analysis of the Metastasys study data

Oncotarget ◽  
2020 ◽  
Vol 11 (32) ◽  
pp. 3026-3034
Author(s):  
Abdelhalim Hussein ◽  
Veit Rohde ◽  
Christina Wolfert ◽  
Silvia Hernandez-Duran ◽  
Ingo Fiss ◽  
...  
Keyword(s):  
Brain Metastases ◽  
Light Microscopy ◽  
White Light ◽  
Cohort Analysis ◽  
Study Data ◽  
Matched Cohort

Light microscopy of wood using sanded surface instead of slides

IAWA Journal ◽  
2021 ◽  
pp. 1-14
Author(s):  
Peter Kitin ◽  
John C. Hermanson ◽  
Hisashi Abe ◽  
Satoshi Nakaba ◽  
Ryo Funada
Keyword(s):  
Light Microscopy ◽  
White Light ◽  
Intact Cell ◽  
Simple Procedure ◽  
Cell Types ◽  
Epifluorescence Microscopy ◽  
Terrestrial Plants ◽  
Xylem Structure ◽  
Micro Features ◽  
Wood Surfaces

Abstract Xylem anatomy is fundamental in studies of the evolution of terrestrial plants, tree ecophysiology, forestry, and wood science. Traditional xylem anatomical studies by light microscopy utilize wood sections. However, the procedures are laborious, and high-quality histological sections have been particularly challenging to achieve from recalcitrant wood species and dry wood material. Modern microscopy offers opportunities for speeding up the xylem anatomical preparations. In this regard, the merits of using a sanded surface for wood anatomical research have been largely overlooked. Sanding of wood surfaces is practiced in dendrochronology and wood identification studies exclusively for the investigation of macro features, such as tree rings, wood porosity, or parenchyma patterns. We conducted microscopic level investigations of sanded surfaces of difficult-to-section high-density woods such as Dalbergia and Quercus species by reflected white light and epifluorescence microscopy. Reflected white light or combinations of reflected light and fluorescence could clearly show xylem micro-features in sanded wood surfaces. The resolution of cell types after sanding with 1000-grit was similar to the resolution obtained by transmitted light microscopy in histological slides. The advantages of sanded wood surfaces compared to traditional wood sections can be summarized as cost- and time-effective sample preparation, large sample area, intact cell walls and tissue structure, preservation of chemical content and extractives, and even focus of the field of view. A simple procedure of wood sanding instead of microscopic slides can be used for xylem microscopy and automatic image analysis of xylem structure.

Quality assurance of HARMS and MOEMS surface structures using confocal white light microscopy

2001 ◽  
Author(s):  
Hans-Joachim Jordan ◽  
Rainer Brodmann ◽  
Marcus Grigat ◽  
Juergen Valentin
Keyword(s):  
Quality Assurance ◽  
Light Microscopy ◽  
White Light ◽  
Surface Structures

scholarly journals Analysis of 5-aminolevulinic acid–induced fluorescence in 55 different spinal tumors

2014 ◽  
Vol 36 (2) ◽  
pp. E11 ◽  
Author(s):  
Matthias Millesi ◽  
Barbara Kiesel ◽  
Adelheid Woehrer ◽  
Johannes A. Hainfellner ◽  
Klaus Novak ◽  
...  
Keyword(s):  
Light Microscopy ◽  
White Light ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Spinal Tumor ◽  
Cns Tumors ◽  
Spinal Tumors ◽  
Total Resection ◽  
Intramedullary Tumors ◽  
Who Grade

Object Subtotal resection (STR) of spinal tumors can result in tumor recurrence. Currently, no clinically reliable marker is available for intraoperative visualization of spinal tumor tissue. Protoporphyrin IX (PpIX) fluorescence induced by 5-aminolevulinic acid (5-ALA) is capable of visualizing malignant gliomas. Fluorescence-guided resections of malignant cerebral gliomas using 5-ALA have resulted in an increased rate of complete tumor removal. Recently, the application of 5-ALA has also been described in the first cases of spinal tumors. Therefore, the aim of this observational study was to systematically investigate 5-ALA–induced fluorescence characteristics in different spinal tumor entities. Methods Three hours before the induction of anesthesia, 5-ALA was administered to patients with different intra- and extradural spinal tumors. In all patients a neurosurgical resection or biopsy of the spinal tumor was performed under conventional white-light microscopy. During each surgery, the presence of PpIX fluorescence was additionally assessed using a modified neurosurgical microscope. At the end of an assumed gross-total resection (GTR) under white-light microscopy, a final inspection of the surgical cavity of fluorescing intramedullary tumors was performed to look for any remaining fluorescing foci. Histopathological tumor diagnosis was established according to the current WHO classification. Results Fifty-two patients with 55 spinal tumors were included in this study. Resection was performed in 50 of 55 cases, whereas 5 of 55 cases underwent biopsy. Gross-total resection was achieved in 37 cases, STR in 5, and partial resection in 8 cases. Protoporphyrin IX fluorescence was visible in 30 (55%) of 55 cases, but not in 25 (45%) of 55 cases. Positive PpIX fluorescence was mainly detected in ependymomas (12 of 12), meningiomas (12 of 12), hemangiopericytomas (3 of 3), and in drop metastases of primary CNS tumors (2 of 2). In contrast, none of the neurinomas (8 of 8), carcinoma metastases (5 of 5), and primary spinal gliomas (3 of 3; 1 pilocytic astrocytoma, 1 WHO Grade II astrocytoma, 1 WHO Grade III anaplastic oligoastrocytoma) revealed PpIX fluorescence. It is notable that residual fluorescing tumor foci were detected and subsequently resected in 4 of 8 intramedullary ependymomas despite assumed GTR under white-light microscopy. Conclusions In this study, 5-ALA–PpIX fluorescence was observed in spinal tumors, especially ependymomas, meningiomas, hemangiopericytomas, and drop metastases of primary CNS tumors. In cases of intramedullary tumors, 5-ALA–induced PpIX fluorescence is a useful tool for the detection of potential residual tumor foci.

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