Cellular Imaging with High-Performance Two-Photon Absorbing Fluorescent Contrast Agents

2006 ◽  
Author(s):  
Kevin D. Belfield ◽  
Katherine J. Schafer-Hales ◽  
Sheng Yao ◽  
Peter K. Frederiksen ◽  
Emmanuel Vrotsos ◽  
...  
Keyword(s):  
Contrast Agents ◽  
High Performance ◽  
Cellular Imaging ◽  
Two Photon

Preparation of aggregation-induced emission dots for long-term two-photon cell imaging

2015 ◽  
Vol 3 (15) ◽  
pp. 3091-3097 ◽  
Author(s):  
Qiang Ye ◽  
Shuangshuang Chen ◽  
Dandan Zhu ◽  
Xuemin Lu ◽  
Qinghua Lu
Keyword(s):  
High Performance ◽  
Cell Imaging ◽  
Cellular Imaging ◽  
Aggregation Induced Emission ◽  
Imaging Capability ◽  
Two Photon

A high-performance two-photon probe with long-term cellular imaging capability was synthesized from an amphiphilic aggregation-induced emission molecule derived from tetraphenylethylene fluorogen.

Synthesis, Two-Photon Absorption, and Cellular Imaging of Two Curcumin Derivatives

2019 ◽  
Vol 89 (12) ◽  
pp. 2577-2583
Author(s):  
W. Zhang ◽  
S. S. Zhou ◽  
G. F. Si ◽  
J. F. Wang ◽  
G. Y. Xu
Keyword(s):  
Cellular Imaging ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Curcumin Derivatives

Efficient two-photon luminescence for cellular imaging using biocompatible nitrogen-doped graphene quantum dots conjugated with polymers

Nanoscale ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Ping-Ching Wu ◽  
Jiu-Yao Wang ◽  
Wen-Lung Wang ◽  
Chia-Yuan Chang ◽  
Chia-Hung Huang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Contrast Agent ◽  
Graphene Quantum Dots ◽  
Cellular Imaging ◽  
Deep Tissue ◽  
Nitrogen Doped ◽  
Two Photon ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

N-doped GQDs conjugated with polymers demonstrate impressive two-photon properties and stability, and can be used as a contrast agent in deep tissue bioimaging.

scholarly journals Reaction-based two-photon probes for in vitro analysis and cellular imaging of monoamine oxidase activity

2012 ◽  
Vol 48 (54) ◽  
pp. 6833 ◽  
Author(s):  
Dokyoung Kim ◽  
Sunderraman Sambasivan ◽  
Hyoseok Nam ◽  
Ki Hean Kim ◽  
Jin Yong Kim ◽  
...  
Keyword(s):  
Monoamine Oxidase ◽  
Oxidase Activity ◽  
Cellular Imaging ◽  
Monoamine Oxidase Activity ◽  
Two Photon ◽  
Vitro Analysis ◽  
In Vitro Analysis

Long-circulating Gd2O3:Yb3+, Er3+ up-conversion nanoprobes as high-performance contrast agents for multi-modality imaging

Biomaterials ◽  
2013 ◽  
Vol 34 (6) ◽  
pp. 1712-1721 ◽  
Author(s):  
Zhen Liu ◽  
Fang Pu ◽  
Sa Huang ◽  
Qinghai Yuan ◽  
Jinsong Ren ◽  
...  
Keyword(s):  
Contrast Agents ◽  
High Performance

scholarly journals Amino-Functionalized Nitrogen-Doped Graphene-Quantum-Dot-Based Nanomaterials with Nitrogen and Amino-Functionalized Group Content Dependence for Highly Efficient Two-Photon Bioimaging

2020 ◽  
Vol 21 (8) ◽  
pp. 2939 ◽  
Author(s):  
Wen-Shuo Kuo ◽  
Chia-Yuan Chang ◽  
Keng-Shiang Huang ◽  
Jui-Chang Liu ◽  
Yu-Ting Shao ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Near Infrared ◽  
Graphene Quantum Dots ◽  
Lower Amount ◽  
Nitrogen Doped ◽  
Two Photon ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene ◽  
Photon Imaging ◽  
Two Photon Imaging

We fabricated nanomaterials comprising amino-functionalized and nitrogen-doped graphene quantum dots (amino-N-GQDs) and investigated their photostability and intrinsic luminescence in the near-infrared spectrum to determine their suitability as contrast agents in two-photon imaging (TPI). We observed that amino-N-GQDs with a higher amount of bonded nitrogen and amino-functionalized groups (6.2%) exhibited superior two-photon properties to those with a lower amount of such nitrogen and groups (4.9%). These materials were conjugated with polymers containing sulfur (polystyrene sulfonate, PSS) and nitrogen atoms (polyethylenimine, PEI), forming amino-N-GQD–PSS–PEI specimens (amino-N-GQD-polymers). The polymers exhibited a high quantum yield, remarkable stability, and notable two-photon properties and generated no reactive oxygen species, rendering them excellent two-photon contrast agents for bioimaging. An antiepidermal growth factor receptor (AbEGFR) was used for labeling to increase specificity. Two-photon imaging (TPI) of amino-N-GQD (6.2%)-polymer-AbEGFR-treated A431 cancer cells revealed remarkable brightness, intensity, and signal-to-noise ratios for each observation at a two-photon excitation power of 16.9 nJ pixel−1 under 30 scans and a three-dimensional (3D) depth of 105 µm, indicating that amino-N-GQD (6.2%)-polymer-AbEGFR-treated cells can achieve two-photon luminescence with 71 times less power required for two-photon autofluorescence (1322.8 nJ pixel−1 with 500 scans) of similar intensity. This economy can minimize photodamage to cells, rendering amino-N-GQD-polymers suitable for noninvasive 3D bioimaging.

Fluorescent AIE dots encapsulated organically modified silica (ORMOSIL) nanoparticles for two-photon cellular imaging

2013 ◽  
Vol 43 (9) ◽  
pp. 1214-1214
Author(s):  
GuangDi CHEN ◽  
Wei QIN ◽  
ZhengPing XU ◽  
Jun QIAN ◽  
ZhenFeng ZHU ◽  
...  
Keyword(s):  
Cellular Imaging ◽  
Modified Silica ◽  
Two Photon ◽  
Organically Modified ◽  
Organically Modified Silica ◽  
Ormosil Nanoparticles
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