In Vivo Imaging of VEGF Expression for Monitoring Molecular Response to Cancer Therapy

2006 ◽  
Author(s):  
Sung K. Chang ◽  
Imran Rizvi ◽  
Nicolas Solban ◽  
Tayyaba Hasan
Keyword(s):  
Cancer Therapy ◽  
In Vivo Imaging ◽  
Molecular Response ◽  
Vegf Expression

scholarly journals A self-illuminating nanoparticle for inflammation imaging and cancer therapy

2019 ◽  
Vol 5 (1) ◽  
pp. eaat2953 ◽  
Author(s):  
Xiaoqiu Xu ◽  
Huijie An ◽  
Dinglin Zhang ◽  
Hui Tao ◽  
Yin Dou ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Microenvironment ◽  
Cancer Therapy ◽  
In Vivo Imaging ◽  
Resonance Energy ◽  
External Irradiation ◽  
Tissue Penetration ◽  
Photodynamic Therapy Of Cancer ◽  
Inflammation Imaging

Nanoparticles have been extensively used for inflammation imaging and photodynamic therapy of cancer. However, the major translational barriers to most nanoparticle-based imaging and therapy applications are the limited depth of tissue penetration, inevitable requirement of external irradiation, and poor biocompatibility of the nanoparticles. To overcome these critical limitations, we synthesized a sensitive, specific, biodegradable luminescent nanoparticle that is self-assembled from an amphiphilic polymeric conjugate with a luminescent donor (luminol) and a fluorescent acceptor [chlorin e6 (Ce6)] for in vivo luminescence imaging and photodynamic therapy in deep tissues. Mechanistically, reactive oxygen species (ROS) and myeloperoxidase generated in inflammatory sites or the tumor microenvironment trigger bioluminescence resonance energy transfer and the production of singlet oxygen (1O2) from the nanoparticle, enabling in vivo imaging and cancer therapy, respectively. This self-illuminating nanoparticle shows an excellent in vivo imaging capability with suitable tissue penetration and resolution in diverse animal models of inflammation. It is also proven to be a selective, potent, and safe antitumor nanomedicine that specifically kills cancer cells via in situ1O2produced in the tumor microenvironment, which contains a high level of ROS.

scholarly journals In vivo imaging of molecular-genetic targets for cancer therapy

Cancer Cell ◽  
2003 ◽  
Vol 3 (4) ◽  
pp. 327-332 ◽  
Author(s):  
Juri Gelovani Tjuvajev ◽  
Ronald G Blasberg
Keyword(s):  
Cancer Therapy ◽  
In Vivo Imaging ◽  
Molecular Genetic

Near‐Infrared‐II Quantum Dots for In Vivo Imaging and Cancer Therapy

Small ◽  
2021 ◽  
pp. 2104567
Author(s):  
Lu‐Lu Chen ◽  
Liang Zhao ◽  
Zhi‐Gang Wang ◽  
Shu‐Lin Liu ◽  
Dai‐Wen Pang
Keyword(s):  
Quantum Dots ◽  
Cancer Therapy ◽  
In Vivo Imaging ◽  
Near Infrared

Self-assembled PEG-IR-780-C13 micelle as a targeting, safe and highly-effective photothermal agent for in vivo imaging and cancer therapy

Biomaterials ◽  
2015 ◽  
Vol 51 ◽  
pp. 184-193 ◽  
Author(s):  
Ahu Yuan ◽  
Xuefeng Qiu ◽  
Xiaolei Tang ◽  
Wei Liu ◽  
Jinhui Wu ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
In Vivo Imaging ◽  
Highly Effective ◽  
Self Assembled

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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