Live cell refractometry using Hilbert phase microscopy

2006 ◽  
Author(s):  
Niyom Lue ◽  
Gabriel Popescu ◽  
Takahiro Ikeda ◽  
Kamran Badizadegan ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
Live Cell ◽  
Phase Microscopy

scholarly journals Live Cell Refractometry Using Hilbert Phase Microscopy and Confocal Reflectance Microscopy†

2009 ◽  
Vol 113 (47) ◽  
pp. 13327-13330 ◽  
Author(s):  
Niyom Lue ◽  
Wonshik Choi ◽  
Gabriel Popescu ◽  
Zahid Yaqoob ◽  
Kamran Badizadegan ◽  
...  
Keyword(s):  
Live Cell ◽  
Phase Microscopy ◽  
Confocal Reflectance Microscopy ◽  
Reflectance Microscopy

Quantitative live cell imaging with tomographic phase microscopy

2009 ◽  
Author(s):  
Wonshik Choi ◽  
Yongjin Sung ◽  
Yongkeun Park ◽  
Christopher Fang-Yen ◽  
Kamran Badizadegan ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Phase Microscopy

scholarly journals Short-coherence off-axis holographic phase microscopy of live cell dynamics

2012 ◽  
Vol 3 (9) ◽  
pp. 2184 ◽  
Author(s):  
Stefan Witte ◽  
Andrius Plauşka ◽  
Margreet C. Ridder ◽  
Laura van Berge ◽  
Huibert D. Mansvelder ◽  
...  
Keyword(s):  
Live Cell ◽  
Cell Dynamics ◽  
Phase Microscopy

scholarly journals Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy

2010 ◽  
Vol 18 (4) ◽  
pp. 3649 ◽  
Author(s):  
Ruei-Yu He ◽  
Chun-Yu Lin ◽  
Yuan-Deng Su ◽  
Kuo-Chih Chiu ◽  
Nan-Shan Chang ◽  
...  
Keyword(s):  
Surface Plasmon ◽  
Cell Membranes ◽  
Live Cell ◽  
Enhanced Fluorescence ◽  
Phase Microscopy ◽  
Plasmon Enhanced Fluorescence

α-Methylene-β-Lactone Probe for Measuring Live-Cell Reactions of Small Molecules

2020 ◽  
Author(s):  
Lei Wang ◽  
Louis Riel ◽  
Bekim Bajrami ◽  
Bin Deng ◽  
Amy Howell ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Live Cell ◽  
Live Cells ◽  
The Novel ◽  
Proteomics Analysis ◽  
Chemical Proteomics ◽  
Tandem Mass Spectra ◽  
Modified Peptides ◽  
Covalent Probes ◽  
Ht 29

The novel use of the α-methylene-β-lactone (MeLac) moiety as a warhead of multiple electrophilic sites is reported. In this study, we demonstrate that a MeLac-alkyne is a competent covalent probe and reacts with diverse proteins in live cells. Proteomics analysis of affinity-enriched samples identifies probe-reacted proteins, resolves their modified peptides/residues, and thus characterizes probe-protein reactions. Unique methods are developed to evaluate confidence in the identification of the reacted proteins and modified peptides. Tandem mass spectra of the peptides reveal that MeLac reacts with nucleophilic cysteine, serine, lysine, threonine, and tyrosine residues, through either Michael addition or acyl addition. A peptide-centric proteomics platform, using MeLac-alkyne as the measurement probe, successfully analyzes the Orlistat selectivity in live HT-29 cells. MeLac is a versatile warhead demonstrating enormous potential to expedite the development of covalent probes and inhibitors in interrogating protein (re)activity. MeLac-empowered platforms in chemical proteomics are widely adaptable for measuring the live-cell action of reactive molecules.

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