Non-Invasive Measurements of Deep Tissue Hemodynamics in Human Skeletal Muscle

2004 ◽  
Author(s):  
G. Yu ◽  
T. Durduran ◽  
G. Lech ◽  
C. Zhou ◽  
C. Britton ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Deep Tissue ◽  
Non Invasive

scholarly journals Magnetic Resonance Spectroscopy as a Non-invasive Method to Quantify Muscle Carnosine in Humans: a Comprehensive Validity Assessment

2019 ◽  
Author(s):  
Vinicius da Eira Silva ◽  
Vitor de Salles Painelli ◽  
Samuel Katsuyuki Shinjo ◽  
Wagner Ribeiro Pereira ◽  
Eduardo Maffud Cilli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Human Skeletal Muscle ◽  
Reference Method ◽  
Resonance Spectroscopy ◽  
Muscle Carnosine ◽  
1H Mrs ◽  
Non Invasive

ABSTRACTCarnosine is a dipeptide abundantly found in human skeletal muscle, cardiac muscle and neuronal cells having numerous properties that confers performance enhancing effects, as well as a wide-range of potential therapeutic applications. A reliable and valid method for tissue carnosine quantification is crucial for advancing the knowledge on biological processes involved with carnosine metabolism. In this regard, proton magnetic resonance spectroscopy (1H-MRS) has been used as a non-invasive alternative to quantify carnosine in human skeletal muscle. However, carnosine quantification by 1H-MRS has some potential limitations that warrant a thorough experimental examination of its validity. The present investigation examined the reliability, accuracy and sensitivity for the determination of muscle carnosine in humans using in vitro and in vivo experiments and comparing it to reference method for carnosine quantification (high-performance liquid chromatography – HPLC). We used in vitro 1H-MRS to verify signal linearity and possible noise sources. Carnosine was determined in the m. gastrocnemius by 1H-MRS and HPLC to compare signal quality and convergent validity. 1H-MRS showed adequate discriminant validity, but limited reliability and poor agreement with a reference method. Low signal amplitude, low signal-to-noise ratio, and voxel repositioning are major sources of error.

T1ρ imaging as a non‐invasive assessment of collagen remodeling and organization in human skeletal muscle after ligamentous injury

2021 ◽  
Author(s):  
Brian Noehren ◽  
Peter A. Hardy ◽  
Anders Andersen ◽  
Camille R. Brightwell ◽  
Jean L. Fry ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Ligamentous Injury ◽  
Collagen Remodeling ◽  
Non Invasive

scholarly journals Repeated exposure to heat stress induces mitochondrial adaptation in human skeletal muscle

2018 ◽  
Vol 125 (5) ◽  
pp. 1447-1455 ◽  
Author(s):  
Paul S. Hafen ◽  
Coray N. Preece ◽  
Jacob R. Sorensen ◽  
Chad R. Hancock ◽  
Robert D. Hyldahl
Keyword(s):  
Skeletal Muscle ◽  
Heat Stress ◽  
Stress Response ◽  
Human Skeletal Muscle ◽  
Deep Tissue ◽  
Heat Stress Response ◽  
Mild Heat ◽  
Tissue Heating ◽  
Mitochondrial Adaptation ◽  
Mitochondrial Adaptations

The heat stress response is associated with several beneficial adaptations that promote cell health and survival. Specifically, in vitro and animal investigations suggest that repeated exposures to a mild heat stress (~40°C) elicit positive mitochondrial adaptations in skeletal muscle comparable to those observed with exercise. To assess whether such adaptations translate to human skeletal muscle, we produced local, deep tissue heating of the vastus lateralis via pulsed shortwave diathermy in 20 men and women ( n = 10 men; n = 10 women). Diathermy increased muscle temperature by 3.9°C within 30 min of application. Immediately following a single 2-h heating session, we observed increased phosphorylation of AMP-activated protein kinase and ERK1/2 but not of p38 MAPK or JNK. Following repeated heat exposures (2 h daily for 6 consecutive days), we observed a significant cellular heat stress response, as heat shock protein 70 and 90 increased 45% and 38%, respectively. In addition, peroxisome proliferator-activated receptor gamma, coactivator-1 alpha and mitochondrial electron transport protein complexes I and V expression were increased after heating. These increases were accompanied by augmentation of maximal coupled and uncoupled respiratory capacity, measured via high-resolution respirometry. Our data provide the first evidence that mitochondrial adaptation can be elicited in human skeletal muscle in response to repeated exposures to mild heat stress. NEW & NOTEWORTHY Heat stress has been shown to elicit mitochondrial adaptations in cell culture and animal research. We used pulsed shortwave diathermy to produce deep tissue heating and explore whether beneficial mitochondrial adaptations would translate to human skeletal muscle in vivo. We report, for the first time, positive mitochondrial adaptations in human skeletal muscle following recurrent heat stress. The results of this study have clinical implications for many conditions characterized by diminished skeletal muscle mitochondrial function.

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