Optical property measurements using broadband reflectance spectroscopy in human tissues before and after photodynamic therapy

2002 ◽  
Author(s):  
Hsing-Wen Wang ◽  
Tim C. Zhu ◽  
Michael Solonenko ◽  
Stephen M. Hahn ◽  
James Metz ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Optical Property ◽  
Reflectance Spectroscopy ◽  
Human Tissues ◽  
Before And After

Stereotactic Intratumoral Photodynamic Therapy for Recurrent Malignant Brain Tumors

Neurosurgery ◽  
1991 ◽  
Vol 29 (5) ◽  
pp. 688-696 ◽  
Author(s):  
Stephen K. Powers ◽  
Sharon S. Cush ◽  
Diana L. Walstad ◽  
Lester Kwock
Keyword(s):  
Photodynamic Therapy ◽  
Magnetic Resonance ◽  
Malignant Gliomas ◽  
Signs And Symptoms ◽  
Resonance Spectroscopy ◽  
Normal Brain ◽  
Hematoporphyrin Derivative ◽  
Neurological Sequelae ◽  
Computed Tomographic ◽  
Before And After

Abstract Photodynamic therapy (PDT) using purified hematoporphyrin derivative and stereotactic intratumorally implanted optical laser fiber(s) was used to treat patients with recurrent malignant gliomas and metastatic melanoma of the brain. Tumor response to PDT was evaluated by recording changes in the volume and pattern of tumor enhancement between computed tomographic and magnetic resonance imaging scans done before and after PDT, metabolic changes in tumor tissue by31 P magnetic resonance spectroscopy, and patient outcome. Toxicity of PDT to brain was evaluated on the basis of changes in the patients' neurological examinations and correlated with changes in brain adjacent to tumor seen on postoperative imaging studies. Dramatic tumor responses to PDT were seen in all gliomas, but no response of tumor to treatment was seen with melanoma. Transient signs and symptoms of increased peritumoral cerebral edema caused by PDT were seen in all patients. Two patients suffered permanent neurological sequelae, monocular blindness and a partial visual field defect, as a result of treatment. Two patients with recurrent anaplastic astrocytomas remain in remission at 45 and 35 weeks after PDT. We conclude that intratumoral photoradiation therapy of hematoporphyrin derivative-photosensitized malignant gliomas effectively produces necrosis of the solid component of malignant gliomas: however, intratumoral photoradiation may not reach the portion of tumor that invades normal brain.

scholarly journals Choroidal Volume Evaluation after Photodynamic Therapy Using New Optical Coherence Tomography Imaging Algorithm

2021 ◽  
Vol 14 (11) ◽  
pp. 1140
Author(s):  
Miki Sato-Akushichi ◽  
Shinji Ono ◽  
Gerd Klose ◽  
Youngseok Song
Keyword(s):  
Photodynamic Therapy ◽  
Choroidal Thickness ◽  
Central Area ◽  
Chronic Central Serous Chorioretinopathy ◽  
Temporal Area ◽  
Short Period ◽  
Before And After ◽  
History Of ◽  
Optical Coherence Tomography Imaging ◽  
Choroidal Volume

To evaluate choroidal volume and thickness changes after photodynamic therapy (PDT) for chronic central serous chorioretinopathy (CSC). Chronic CSC eyes with a history of PDT were selected. Average choroidal volume, average choroidal thickness, the maximum and minimum choroidal thickness of the macula irradiated area and peripheral non-irradiated areas before and after one and three months of treatment were examined. A total of 14 patients with chronic CSC and 9 controls without any eye pathology were enrolled. The mean choroidal volume in CSC before and, and after one and three months of treatment were 2.36 (standard deviation: 0.70), 1.90 (0.69), 1.86 (0.66) mm3 for the central area, 1.25 (0.38), 1.14 (0.35), 1.13 (0.34) mm3 for superior nasal area, 1.47 (0.41), 1.28 (0.43), 1.26 (0.43) mm3 for superior temporal area, 1.07 (0.49), 0.95 (0.38), 0.93 (0.35) mm3 for inferior nasal area, 1.17 (0.38), 1.04 (0.32), 1.03 (0.33) mm3 for inferior temporal area. This study revealed the choroidal volume changes in a short period after PDT and a decrease in unirradiated choroidal volume was also shown after the treatment. The algorithm provided on the ARI Network enables to evaluate the choroidal changes quantitatively and qualitatively.

582 Cyclooxygenase-2 Expression in Esophageal Epithelium Before and After Photodynamic Therapy for Barrett's Esophagus

2008 ◽  
Vol 134 (4) ◽  
pp. A-79 ◽  
Author(s):  
Patrick S. Yachimski ◽  
Mari Mino-Kenudson ◽  
Margaret E. Sherwood ◽  
William P. Puricelli ◽  
Norman S. Nishioka ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Cyclooxygenase 2 ◽  
Esophageal Epithelium ◽  
Before And After

Simplified Scatchard-Plot Assay for Estrogen Receptor in Human Breast Tumor

1975 ◽  
Vol 21 (12) ◽  
pp. 1725-1730 ◽  
Author(s):  
Roy B Johnson ◽  
Robert M Nakamura ◽  
Rose Mary Libby
Keyword(s):  
Estrogen Receptor ◽  
Breast Tumor ◽  
Tumor Tissue ◽  
Competitive Inhibition ◽  
Scatchard Plot ◽  
Human Tissues ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Pregnant Rabbit ◽  
Before And After

Abstract In this two-point Scatchard-plot assay, with which a test of competitive inhibition is combined, the sample is mechanically homogenized in a buffer containing dithiothreitol, ultracentrifuged to obtain a fat-free cytosol, the protein content of which is then adjusted, and free and bound labeled estradiol are separated with dextrancoated charcoal after overnight incubation. We tested the method for precision and reliability by assaying such cytosols from pregnant rabbit uteri before and after dilution with kidney cytosol, and by assaying several other target and nontarget animal and human tissues. The Scatchard plot data were more reliable than tests for percent inhibition of binding by a competitor (diethylstilbestrol). For a tumor tissue to be judged positive it must bind at least 8 fmol of estradiol per milligram of protein and have a Kd of 0.1 to 5 x 10-10 mol/liter. Some nontarget tissues showed less than 70% inhibition by 104-fold concentrations (over labeled estradiol) of inhibitor. Of 19 breast-tumor specimens, seven were found to be positive.

Inspecting historical vaulted ceilings by means of physical and chemical analyses: an integrated approach combining active infrared thermography and reflectance spectroscopy

2020 ◽  
Vol 62 (3) ◽  
pp. 144-151 ◽  
Author(s):  
S Sfarra ◽  
E Cheilakou ◽  
P Theodorakeas ◽  
C Ibarra-Castanedo ◽  
H Zhang ◽  
...  
Keyword(s):  
Infrared Thermography ◽  
Near Infrared ◽  
Diffuse Reflectance Spectroscopy ◽  
Central Italy ◽  
Integrated Approach ◽  
Reflectance Spectroscopy ◽  
Fibre Optics ◽  
Buried Structures ◽  
Before And After ◽  
Physical And Chemical

The present study discusses the experimental physicochemical results obtained from the historical vaulted ceilings of an ancient church located in central Italy. Infrared thermography (IRT) in the active configuration was used to map subsurface defects caused by a seismic event and to discover buried structures, while the visible and near-infrared (VIS-NIR) fibre-optics diffuse reflectance spectroscopy (FORS) technique was applied to identify the pigments of wall paintings decorating the vault. Historical photographs are useful to readers in order to clarify the state of conservation before and after the earthquake that took place in 2009. The combination of the experimental results can be useful in restoration processes.

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