Near Infrared Raman Spectroscopy for Cancer Detection in vivo

2002 ◽  
Author(s):  
Anita Mahadevan-Jansen ◽  
Amy Robichaux ◽  
Chad Lieber ◽  
Heidi Shappell ◽  
Darryl Ellis ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Detection ◽  
Near Infrared

RAMAN SPECTROSCOPY FOR IN VIVO TISSUE ANALYSIS AND DIAGNOSIS, FROM INSTRUMENT DEVELOPMENT TO CLINICAL APPLICATIONS

2008 ◽  
Vol 01 (01) ◽  
pp. 95-106 ◽  
Author(s):  
HAISHAN ZENG ◽  
JIANHUA ZHAO ◽  
MICHAEL SHORT ◽  
DAVID I. MCLEAN ◽  
STEPHEN LAM ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectra ◽  
Cancer Detection ◽  
Tissue Characterization ◽  
Near Infrared ◽  
Skin Diseases ◽  
Clinical Applications ◽  
Fiber Array ◽  
Raman Probe

Raman spectroscopy is a noninvasive, nondestructive analytical method capable of determining the biochemical constituents based on molecular vibrations. It does not require sample preparation or pretreatment. However, the use of Raman spectroscopy for in vivo clinical applications will depend on the feasibility of measuring Raman spectra in a relatively short time period (a few seconds). In this work, a fast dispersive-type near-infrared (NIR) Raman spectroscopy system and a skin Raman probe were developed to facilitate real-time, noninvasive, in vivo human skin measurements. Spectrograph image aberration was corrected by a parabolic-line fiber array, permitting complete CCD vertical binning, thereby yielding a 16-fold improvement in signal-to-noise ratio. Good quality in vivo skin NIR Raman spectra free of interference from fiber fluorescence and silica Raman scattering can be acquired within one second, which greatly facilitates practical noninvasive tissue characterization and clinical diagnosis. Currently, we are conducting a large clinical study of various skin diseases in order to develop Raman spectroscopy into a useful tool for non-invasive skin cancer detection. Intermediate data analysis results are presented. Recently, we have also successfully developed a technically more challenging endoscopic Laser-Raman probe for early lung cancer detection. Preliminary in vivo results from endoscopic lung Raman measurements are discussed.

scholarly journals Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 922
Author(s):  
William Querido ◽  
Shital Kandel ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Biological Tissues ◽  
Label Free ◽  
Connective Tissues ◽  
Bone Properties ◽  
Composition And Structure

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

In vivo detection of cervical dysplasia with near-infrared Raman spectroscopy

2002 ◽  
Author(s):  
Amy Robichaux ◽  
Chad A. Lieber ◽  
Heidi Shappell ◽  
Beth Huff ◽  
Howard Jones III ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Cervical Dysplasia

Combining near-infrared-excited autofluorescence and Raman spectroscopy improves in vivo diagnosis of gastric cancer

2011 ◽  
Vol 26 (10) ◽  
pp. 4104-4110 ◽  
Author(s):  
Mads Sylvest Bergholt ◽  
Wei Zheng ◽  
Kan Lin ◽  
Khek Yu Ho ◽  
Ming Teh ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Raman Spectroscopy ◽  
Near Infrared ◽  
In Vivo Diagnosis

Real-time In Vivo Tissue Raman Spectroscopy for Early Cancer Detection

2016 ◽  
Author(s):  
Haishan Zeng ◽  
Jianhua Zhao ◽  
Michael A. Short ◽  
David I. McLean ◽  
Stephen Lam ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Cancer Detection ◽  
Early Cancer ◽  
Early Cancer Detection

Assessment of ex-vivo and in-vivo near-infrared Raman spectroscopy for the classification of dysplasia within Barrett's esophagus

2000 ◽  
Author(s):  
Martin G. Shim ◽  
Louis-Michel Wong Kee Song ◽  
Norman E. Marcon ◽  
Shirley Hassaram ◽  
Brian C. Wilson
Keyword(s):  
Raman Spectroscopy ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Near Infrared ◽  
Ex Vivo

Raman Signal Enhancement in Deep Spectroscopy of Turbid Media

2007 ◽  
Vol 61 (8) ◽  
pp. 845-854 ◽  
Author(s):  
P. Matousek
Keyword(s):  
Raman Spectroscopy ◽  
Laser Radiation ◽  
Laser Beam ◽  
Near Infrared ◽  
Optical Element ◽  
Disease Diagnosis ◽  
Pharmaceutical Products ◽  
Turbid Media ◽  
Raman Signal

A new, passive method for enhancing spontaneous Raman signals for the spectroscopic investigation of turbid media is presented. The main areas to benefit are transmission Raman and spatially offset Raman spectroscopy approaches for deep probing of turbid media. The enhancement, which is typically several fold, is achieved using a multilayer dielectric optical element, such as a bandpass filter, placed within the laser beam over the sample. This element prevents loss of the photons that re-emerge from the medium at the critical point where the laser beam enters the sample, the point where major photon loss occurs. This leads to a substantial increase of the coupling of laser radiation into the sample and consequently an enhanced laser photon–medium interaction process. The method utilizes the angular dependence of dielectric optical elements on impacting photon direction with its transmission spectral profile shifting to the blue with increase in the deviation of photons away from normal incidence. This feature enables it to act as a unidirectional mirror passing a semi-collimated laser beam through unhindered from one side, and at the other side, reflecting photons emerging from the sample at random directions back into it with no restrictions to the detected Raman signal. With substantial restrictions to the spectral range, the concept can also be applied to conventional backscattering Raman spectroscopy. The use of additional reflective elements around the sample to enhance the Raman signal further is also discussed. The increased signal strength yields higher signal quality, a feature important in many applications. Potential uses include sensitive noninvasive disease diagnosis in vivo, security screening, and quality control of pharmaceutical products. The concept is also applicable in an analogous manner to other types of analytical methods such as fluorescence or near-infrared (NIR) absorption spectroscopy of turbid media or it can be used to enhance the effectiveness of the coupling of laser radiation into tissue in applications such as photodynamic therapy for cancer treatment.

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