Correlative Studies of Autofluorescence, Immuno/histopathology and Infrastructures of Ex Vivo Tissues and Primary Cultured Epithelial Cells from Human Normal, Preneoplastic and Neoplastic Colorectal Mucosa.

1999 ◽  
Author(s):  
Ralph S. DaCosta ◽  
Helene Andersson ◽  
Brian C. Wilson ◽  
Maria Cirocco ◽  
Shirley Hassaram ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ex Vivo ◽  
Colorectal Mucosa ◽  
Cultured Epithelial Cells ◽  
Correlative Studies ◽  
Ex Vivo Tissues

Vimentin organizing centre in cultured epithelial cells

Pathology ◽  
1984 ◽  
Vol 16 (4) ◽  
pp. 393-395 ◽  
Author(s):  
John S. Pedersen ◽  
J.R. Underwood ◽  
B.H. Toh
Keyword(s):  
Epithelial Cells ◽  
Cultured Epithelial Cells

scholarly journals Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Kristina Andelid ◽  
Karolina Öst ◽  
Anders Andersson ◽  
Esha Mohamed ◽  
Zala Jevnikar ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Chronic Bronchitis ◽  
Cross Talk ◽  
Alveolar Macrophages ◽  
Ex Vivo ◽  
Bronchial Epithelial Cells ◽  
Steroid Resistance ◽  
Bronchial Epithelial ◽  
Steroid Resistant ◽  
Copd Patients

Abstract Background Patients with chronic obstructive pulmonary disease (COPD) frequently suffer from chronic bronchitis (CB) and display steroid-resistant inflammation with increased sputum neutrophils and macrophages. Recently, a causal link between mucus hyper-concentration and disease progression of CB has been suggested. Methods In this study, we have evaluated the steroid sensitivity of purified, patient-derived sputum and alveolar macrophages and used a novel mechanistic cross-talk assay to examine how macrophages and bronchial epithelial cells cross-talk to regulate MUC5B production. Results We demonstrate that sputum plug macrophages isolated from COPD patients with chronic bronchitis (COPD/CB) are chronically activated and only partially respond to ex vivo corticosteroid treatment compared to alveolar macrophages isolated from lung resections. Further, we show that pseudo-stratified bronchial epithelial cells grown in air–liquid-interface are inert to direct bacterial lipopolysaccharide stimulation and that macrophages are able to relay this signal and activate the CREB/AP-1 transcription factor complex and subsequent MUC5B expression in epithelial cells through a soluble mediator. Using recombinant protein and neutralizing antibodies, we identified a key role for TNFα in this cross-talk. Conclusions For the first time, we describe ex vivo pharmacology in purified human sputum macrophages isolated from chronic bronchitis COPD patients and identify a possible basis for the steroid resistance frequently seen in this population. Our data pinpoint a critical role for chronically activated sputum macrophages in perpetuating TNFα-dependent signals driving mucus hyper-production. Targeting the chronically activated mucus plug macrophage phenotype and interfering with aberrant macrophage-epithelial cross-talk may provide a novel strategy to resolve chronic inflammatory lung disease.

scholarly journals Eviscerated Corneas as Tissue Source for Ex Vivo Expansion of Limbal Epithelial Cells on Platelet-Rich Plasma Gels

2016 ◽  
Vol 41 (12) ◽  
pp. 1543-1547 ◽  
Author(s):  
Leonard G. Heydenrych ◽  
Donald F. du Toit ◽  
Colleen M. Aldous
Keyword(s):  
Epithelial Cells ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
Ex Vivo Expansion ◽  
Tissue Source

Cultured epithelial cells derived from human foetal pancreas as a model for the study of cystic fibrosis: further analyses on the origins and nature of the cell types

1988 ◽  
Vol 90 (1) ◽  
pp. 73-77
Author(s):  
A. Harris ◽  
L. Coleman
Keyword(s):  
Cystic Fibrosis ◽  
Tissue Culture ◽  
Epithelial Cells ◽  
Culture System ◽  
Cultured Cells ◽  
Cell Types ◽  
Cultured Epithelial Cells ◽  
Different Cell Types ◽  
Foetal Pancreas

The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results support the view that the cultured cells are ductal in origin and nature. Pancreatic epithelial cell cultures have also been established from foetuses with cystic fibrosis.

Drug-induced alterations of cytokeratin organization in cultured epithelial cells

Science ◽  
1983 ◽  
Vol 219 (4584) ◽  
pp. 501-503 ◽  
Author(s):  
L. Knapp ◽  
W. O'Guin ◽  
R. Sawyer
Keyword(s):  
Epithelial Cells ◽  
Drug Induced ◽  
Cultured Epithelial Cells

scholarly journals Expression of Matrix Metalloproteinase Gelatinases A and B by Cultured Epithelial Cells from Human Bronchial Explants

1996 ◽  
Vol 271 (26) ◽  
pp. 15580-15589 ◽  
Author(s):  
Pin Mei Yao ◽  
Jean-Marie Buhler ◽  
Marie Pia d'Ortho ◽  
François Lebargy ◽  
Christophe Delclaux ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Matrix Metalloproteinase ◽  
Cultured Epithelial Cells ◽  
Gelatinases A And B
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