Image deconvolution for confocal laser scanning microscopy using constrained total variation with a gradient field

2019 ◽  
Vol 58 (14) ◽  
pp. 3754 ◽  
Author(s):  
Tao He ◽  
Yasheng Sun ◽  
Jin Qi ◽  
Jie Hu ◽  
Haiqing Huang
Keyword(s):  
Total Variation ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Gradient Field ◽  
Confocal Laser ◽  
Image Deconvolution

scholarly journals Total Variation-Based Noise Reduction Image Processing Algorithm for Confocal Laser Scanning Microscopy Applied to Activity Assessment of Early Carious Lesions

2020 ◽  
Vol 10 (12) ◽  
pp. 4090
Author(s):  
Hee-Eun Kim ◽  
Seong-Hyeon Kang ◽  
Kyuseok Kim ◽  
Youngjin Lee
Keyword(s):  
Noise Reduction ◽  
Total Variation ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Activity Assessment ◽  
Carious Lesions

The confocal laser scanning microscopy (CLSM) system has been widely used to analyze early carious lesions with fluorescent ligands in dental imaging. This system can be used to examine the physiological condition of cellular colonization in the tooth structure. However, the undesirable noise in CLSM images hinders accurate activity assessment of early carious lesions. To address this limitation, a total variation (TV)-based noise reduction algorithm with good edge preservation was developed, and its applicability to medical tooth specimen images obtained with CLSM was verified. To evaluate the imaging performance, the proposed algorithm was compared with conventional filtering methods in terms of the normalized noise power spectrum, contrast-to-noise ratio, and coefficient of variation. The results indicate that the proposed algorithm achieved better noise performance and fine-detail preservation, in comparison with the conventional methods.

ACTIVATED SLUDGE FLOC CHARACTERIZATION BY CONFOCAL LASER SCANNING MICROSCOPY

2012 ◽  
Vol 11 (3) ◽  
pp. 669-674 ◽  
Author(s):  
Szabolcs Szilveszter ◽  
Botond Raduly ◽  
Szilard Bucs ◽  
Beata Abraham ◽  
Szabolcs Lanyi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

scholarly journals Bacterial Colonization and Proliferation in Furcal Perforations Repaired by Different Materials: A Confocal Laser Scanning Microscopy Study

2021 ◽  
Vol 11 (8) ◽  
pp. 3403
Author(s):  
Shlomo Elbahary ◽  
Sohad Haj Yahya ◽  
Cemre Koç ◽  
Hagay Shemesh ◽  
Eyal Rosen ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Bacterial Colonization ◽  
Microscopy Study ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Repair Material ◽  
Dentinal Tubules

Following furcal perforation, bacteria may colonize the defect and cause inflammation and periodontal destruction. This study used confocal laser scanning microscopy (CLSM) to evaluate Enterococcus faecalis colonization and proliferation in furcal perforations repaired with different materials. Furcal perforations created in 55 extracted human mandibular molars were repaired using either MTA-Angelus, Endocem, or Biodentine and coronally subjected to E. faecalis suspension for 21 days. The specimens were then stained using a LIVE/DEAD Viability Kit and visualized by CLSM. The minimum and maximum depths of bacterial penetration into the dentinal tubules were 159 and 1790 μM, respectively, with a mean of 713 μM. There were significantly more live than dead bacteria inside the dentinal tubules (p = 0.0023) in all groups, and all three repair materials exhibited a similarly sized stained area (p = 0.083). However, there were significant differences in the numbers of dead bacteria at the circumference of the perforation defect (p = 0.0041), with a significantly higher ratio of live to dead bacteria in the MTA-Angelus group (p = 0.001). Following perforation repair, bacteria may colonize the interface between the repair material and dentin and may penetrate through the dentinal tubules. The type of repair material has a significant effect on the viability of the colonizing bacteria.

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