scholarly journals Automatic numerical focus plane estimation indigital holographic microscopy usingcalibration beads

2022 ◽  
Author(s):  
Dylan Brault ◽  
Corinne Fournier ◽  
Thomas Olivier ◽  
Nicolas Faure ◽  
Sophie Dixneuf ◽  
...  
Keyword(s):  
Holographic Microscopy ◽  
Focus Plane

3-D optical transfer in excitation field synthesis microscopes

1995 ◽  
Vol 53 ◽  
pp. 64-65
Author(s):  
Vijay Krishnamurthi ◽  
Brent Bailey ◽  
Frederick Lanni
Keyword(s):  
Standing Wave ◽  
Spatial Information ◽  
3D Structure ◽  
Complete Recovery ◽  
Weighting Factor ◽  
Optical Transfer Function ◽  
Excitation Field ◽  
Optical Transfer ◽  
Set Up ◽  
Focus Plane

Excitation field synthesis (EFS) refers to the use of an interference optical system in a direct-imaging microscope to improve 3D resolution by axially-selective excitation of fluorescence within a specimen. The excitation field can be thought of as a weighting factor for the point-spread function (PSF) of the microscope, so that the optical transfer function (OTF) gets expanded by convolution with the Fourier transform of the field intensity. The simplest EFS system is the standing-wave fluorescence microscope, in which an axially-periodic excitation field is set up through the specimen by interference of a pair of collimated, coherent, s-polarized beams that enter the specimen from opposite sides at matching angles. In this case, spatial information about the object is recovered in the central OTF passband, plus two symmetric, axially-shifted sidebands. Gaps between these bands represent "lost" information about the 3D structure of the object. Because the sideband shift is equal to the spatial frequency of the standing-wave (SW) field, more complete recovery of information is possible by superposition of fields having different periods. When all of the fields have an antinode at a common plane (set to be coincident with the in-focus plane), the "synthesized" field is peaked in a narrow infocus zone.

Interferential seeing monitor, a seeing monitor for atmospheric turbulence studies: calibration with the differential image motion monitor

2020 ◽  
Vol 500 (2) ◽  
pp. 1884-1888
Author(s):  
Mohammed Sabil ◽  
A Habib ◽  
Z Benkhaldoun
Keyword(s):  
Structure Function ◽  
Atmospheric Turbulence ◽  
Wave Structure ◽  
Image Motion ◽  
Phase Fluctuations ◽  
Amplitude Fluctuations ◽  
Focus Plane ◽  
Cross Calibration ◽  
Double Slit ◽  
Good Agreement

ABSTRACT In this work, we aim to calibrate an interferential seeing monitor (ISM), which is a testing instument used at astronomical sites. Its method is based on the study of the diffraction pattern produced by a Young’s double-slit at the focus plane of a telescope. This method allows us to obtain the wave structure function by taking into account both phase and amplitude fluctuations of the light wavefront. A phase seeing εϕ was assigned to phase fluctuations and an amplitude seeing εχ was assigned to amplitude fluctuations (scintillation phenomenon), which allows us to obtain both phase and amplitude fluctuations. The feasibility of the ISM method was demonstrated by numerical simulations presented in a previous work. In this work, we have conducted a cross-calibration campaign of the ISM with a differential image motion monitor (DIMM) over 16 nights at the Oukaimeden and Atlas Golf Marrakech Observatories. The goal of this campaign was to study the reliability of this new method. In this paper, we present the calibration measurements and a comparison between the seeing measured by the ISM (εϕ, εχ) and that obtained by the DIMM (εdimm). These results show good agreement between the phase- eeing εϕ and εdimm.

scholarly journals A Multi Camera and Multi Laser Calibration Method for 3D Reconstruction of Revolution Parts

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 765
Author(s):  
Hugo Álvarez ◽  
Marcos Alonso ◽  
Jairo R. Sánchez ◽  
Alberto Izaguirre
Keyword(s):  
3D Reconstruction ◽  
Calibration Method ◽  
Laser Plane ◽  
Calibration Methods ◽  
Laser Calibration ◽  
Calibration Object ◽  
Focus Plane ◽  
Chessboard Pattern ◽  
Line Patterns

This paper describes a method for calibrating multi camera and multi laser 3D triangulation systems, particularly for those using Scheimpflug adapters. Under this configuration, the focus plane of the camera is located at the laser plane, making it difficult to use traditional calibration methods, such as chessboard pattern-based strategies. Our method uses a conical calibration object whose intersections with the laser planes generate stepped line patterns that can be used to calculate the camera-laser homographies. The calibration object has been designed to calibrate scanners for revolving surfaces, but it can be easily extended to linear setups. The experiments carried out show that the proposed system has a precision of 0.1 mm.

Antifouling mechanism of natural product-based coatings investigated by digital holographic microscopy

2021 ◽  
Vol 84 ◽  
pp. 200-207
Author(s):  
Jiansen Pan ◽  
Qingmei Peng ◽  
Guoliang Zhang ◽  
Qingyi Xie ◽  
Xiangjun Gong ◽  
...  
Keyword(s):  
Natural Product ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy
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