Three-observation-window time-gated algorithm for fluorescence lifetime detection

2020 ◽  
Vol 59 (9) ◽  
pp. 2739 ◽  
Author(s):  
Sihui Zhu ◽  
Yue Xu ◽  
Ding Li
Keyword(s):  
Fluorescence Lifetime ◽  
Observation Window ◽  
Fluorescence Lifetime Detection

scholarly journals Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing

IEEE Access ◽  
2019 ◽  
Vol 7 ◽  
pp. 111944-111953
Author(s):  
Liping Wei ◽  
Hoi Man Leung ◽  
Yi Tian ◽  
Pik Kwan Lo ◽  
Derek Ho
Keyword(s):  
Fluorescence Lifetime ◽  
Liquid Core ◽  
Fully Integrated ◽  
Liquid Core Waveguide ◽  
Fluorescence Lifetime Detection

Multiplex Single Strand Conformation Polymorphism Analysis by Capillary Electrophoresis with On-the-Fly Fluorescence Lifetime Detection

2005 ◽  
Vol 59 (3) ◽  
pp. 335-339 ◽  
Author(s):  
Tara Morcone Snyder ◽  
Linda B. McGown
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescence Lifetime ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Polymorphism Analysis ◽  
Wild Type ◽  
G20210a Mutation ◽  
Prothrombin Gene ◽  
Fluorescence Lifetime Detection ◽  
Conformation Polymorphism

This paper describes the use of on-the-fly fluorescence lifetime detection (OFLD) for multiplex single strand conformation polymorphism (SSCP) analysis by capillary electrophoresis (CE). The dye labels studied for multiplex SSCP-OFLD-CE analyses included RG, NBD, and BODIPY-FL. The dyes were first investigated for a model system of “Wild Type” and “Mutant” 43-base fragments designed to vary by a single A/T substitution. Two dye pairs, BODIPY-FL/RG and BODIPY-FL/NBD, were then used to detect the G20210A mutation in the human prothrombin gene. Mobility correction was required for the BODIPY-FL/RG system. Three “blind” analyses were performed of three mixtures that combined a control fragment (wild type-BODIPY-FL) with two “unknown” fragments selected among four possibilities (wild type or mutant labeled with NBD or RG). In each multiplex analysis, the “origin” of the unknown fragments was correctly identified on the basis of fluorescence lifetime of the dye label and the presence or absence of the mutation was correctly determined on the basis of conformation-induced differences in migration time.

On-the-Fly Fluorescence Lifetime Detection of Humic Substances in Capillary Electrophoresis

2003 ◽  
Vol 57 (3) ◽  
pp. 256-265 ◽  
Author(s):  
Joseph D. Hewitt ◽  
Linda B. McGown
Keyword(s):  
Humic Substances ◽  
Fluorescence Lifetime ◽  
Spectral Characteristics ◽  
Emission Spectra ◽  
Fulvic Acids ◽  
Fluorescence Decay ◽  
Fluorescence Excitation ◽  
Lifetime Analysis ◽  
Decay Profile ◽  
Fluorescence Lifetime Detection

On-the-fly fluorescence lifetime detection was investigated as a tool for studying humic substances in capillary zone electrophoresis (CZE). Humic substances are complex, heterogeneous mixtures of natural products that tend to migrate in a single, broad CZE peak. The intrinsic fluorescence lifetime of five humic substances from the International Humic Substances Society (IHSS) was monitored using excitation at 488 or 364 nm to produce intensity-lifetime electropherograms for each of the substances. Each frequency-domain lifetime measurement, collected at sub-second intervals during the CZE run, contains the equivalent of a complete decay profile. Lifetime analysis of each decay profile was used to construct a lifetime-resolved electropherogram for each lifetime component, from which the variation in relative intensity contributions of each lifetime across the broad CZE peak could be determined. Absorption spectra, fluorescence excitation–emission spectra, and lifetime profiles of batch solutions of the samples were determined as well. It was found that, whereas absorption and fluorescence spectral characteristics tended to discriminate between humic acids and fulvic acids, the batch solution lifetime profiles discriminated instead between samples from different sources, regardless of fraction. On-the-fly lifetime detection provided a more detailed view of the fluorescence decay of the samples, including greater resolution of lifetimes for two of the fulvic acids and greater discrimination among samples based on lifetime profiles across the CZE peaks.

Multilayered MOEMS Tunable Spectrometer for Fluorescence Lifetime Detection

2010 ◽  
Vol 22 (7) ◽  
pp. 486-488
Author(s):  
Joe Fu-Jiou Lo ◽  
Pramod Butte ◽  
Qiyin Fang ◽  
Shih-Jui Chen ◽  
Thanassis Papaioanou ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Detection
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