Hybrid application of complex wavefront shaping optical coherence tomography and optical clearing agents for the penetration depth enhancement

2015 ◽  
Author(s):  
Hyeonseung Yu ◽  
Jaehyun Peter Lee ◽  
YoungJu Jo ◽  
Yong Jeong ◽  
Varley V. Tuchin ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Penetration Depth ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Wavefront Shaping ◽  
Depth Enhancement

scholarly journals Collaborative effects of wavefront shaping and optical clearing agent in optical coherence tomography

2016 ◽  
Vol 21 (12) ◽  
pp. 121510 ◽  
Author(s):  
Hyeonseung Yu ◽  
Peter Lee ◽  
YoungJu Jo ◽  
KyeoReh Lee ◽  
Valery V. Tuchin ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Wavefront Shaping

Monitoring of the Process of Immersion Optical Clearing of Collagen Bundles Using Optical Coherence Tomography

2019 ◽  
Vol 127 (2) ◽  
pp. 359-367 ◽  
Author(s):  
M. E. Shvachkina ◽  
D. D. Yakovlev ◽  
E. N. Lazareva ◽  
A. B. Pravdin ◽  
D. A. Yakovlev
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Optical Clearing

scholarly journals Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents

2020 ◽  
Vol 245 (18) ◽  
pp. 1629-1636
Author(s):  
Ruiming Kong ◽  
Wenjuan Wu ◽  
Rui Qiu ◽  
Lei Gao ◽  
Fengxian Du ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Light Attenuation ◽  
Anterior Segment ◽  
Posterior Segment ◽  
Glycerol Solution ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Imaging Depth

Optical coherence tomography has become an indispensable diagnostic tool in ophthalmology for imaging the retina and the anterior segment of the eye. However, the imaging depth of optical coherence tomography is limited by light attenuation in tissues due to optical scattering and absorption. In this study of rabbit eye both ex vivo and in vivo, optical coherence tomography imaging depth of the anterior and posterior segments of the eye was extended by using optical clearing agents to reduce multiple scattering. The sclera, the iris, and the ciliary body were clearly visualized by direct application of glycerol at an incision on the conjunctiva, and the posterior boundary of sclera and even the deeper tissues were detected by submerging the posterior segment of eye in glycerol solution ex vivo or by retro-bulbar injection of glycerol in vivo. The ex vivo rabbit eyes recovered to their original state in 60 s after saline-wash treatment, and normal optical coherence tomography images of the posterior segment of the sample eyes proved the self-recovery of in vivo performance. Signal intensities of optical coherence tomography images obtained before and after glycerol treatment were compared to analysis of the effect of optical clearing. To the best of our knowledge, this is the first study for imaging depth extension of optical coherence tomography in both the anterior and posterior segments of eye by using optical clearing agents.

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