Multi-Modal Quantitative Imaging of Genetically Modified Tumor Cells Utilizing Digital Holographic Microscopy

2016 ◽  
Author(s):  
Björn Kemper ◽  
Burkhard Greve ◽  
Martin Götte ◽  
Steffi Ketelhut
Keyword(s):  
Tumor Cells ◽  
Genetically Modified ◽  
Quantitative Imaging ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

scholarly journals Label-free fingerprinting of tumor cells in bulk flow using inline digital holographic microscopy

2017 ◽  
Vol 8 (2) ◽  
pp. 536 ◽  
Author(s):  
Dhananjay Kumar Singh ◽  
Caroline C. Ahrens ◽  
Wei Li ◽  
Siva A. Vanapalli
Keyword(s):  
Tumor Cells ◽  
Bulk Flow ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy

Morphological Changes in Malignant Tumor Cells at Photodynamic Treatment Assessed by Digital Holographic Microscopy

2019 ◽  
Vol 13 (3) ◽  
pp. 394-400 ◽  
Author(s):  
A. A. Zhikhoreva ◽  
A. V. Belashov ◽  
D. A. Gorbenko ◽  
N. A. Avdonkina ◽  
I. A. Baldueva ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Morphological Changes ◽  
Digital Holographic Microscopy ◽  
Photodynamic Treatment ◽  
Holographic Microscopy

Investigation of living pancreas tumor cells by digital holographic microscopy

2006 ◽  
Vol 11 (3) ◽  
pp. 034005 ◽  
Author(s):  
Björn Kemper ◽  
Daniel Carl ◽  
Jürgen Schnekenburger ◽  
Ilona Bredebusch ◽  
Marcus Schäfer ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Digital Holographic Microscopy ◽  
Pancreas Tumor ◽  
Holographic Microscopy

scholarly journals Subpopulations of Organoid-Forming Cells Have Different Motility

2020 ◽  
Vol 10 (13) ◽  
pp. 4673
Author(s):  
David Gomez Jimenez ◽  
Sofia Carreira Santos ◽  
Lennart Greiff ◽  
Kersti Alm ◽  
Malin Lindstedt
Keyword(s):  
Tumor Cells ◽  
Three Dimensional ◽  
Cell Aggregation ◽  
Digital Holographic Microscopy ◽  
Optical Parameters ◽  
Label Free ◽  
Light Passing ◽  
Tumorigenic Potential ◽  
Holographic Microscopy ◽  
Tumor Organoids

Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 105 µm3. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44+ cells displayed greater motility and tumor-forming capacity than those of CD44− cells. These results were in line with previous reports highlighting increased invasive and tumorigenic potential in tumor cells expressing high levels of CD44. Our method provides insight into the formation of tumor organoids, and could be used to assess stemness-associated biomarkers and drug screenings on the basis of tumor organoids.

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