Geldanamycin reduces proliferation of highly pathogenic H5N1 avian influenza virus <italic>in vivo</italic>

2013 ◽  
Vol 58 (11) ◽  
pp. 1021-1026 ◽  
Author(s):  
BaoHua ZHAO ◽  
Fei ZHONG ◽  
Yi HU ◽  
HongXuan HE ◽  
PengPeng LIU
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Pathogenic H5n1

Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus

Gene ◽  
2015 ◽  
Vol 559 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Periyasamy Vijayakumar ◽  
Anamika Mishra ◽  
Pradip B. Ranaware ◽  
Atul P. Kolte ◽  
Diwakar D. Kulkarni ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Pathogenic H5n1

scholarly journals Highly Pathogenic H5N1 Avian Influenza Virus Induces Extracellular Ca2+ Influx, Leading to Apoptosis in Avian Cells

2010 ◽  
Vol 84 (6) ◽  
pp. 3068-3078 ◽  
Author(s):  
Mayo Ueda ◽  
Tomo Daidoji ◽  
Anariwa Du ◽  
Cheng-Song Yang ◽  
Madiha S. Ibrahim ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Influenza A Virus ◽  
Influenza A ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Infected Cells ◽  
Pathogenic H5n1

ABSTRACT In this study, we show that the highly pathogenic H5N1 avian influenza virus (AIV) (A/crow/Kyoto/53/04 and A/chicken/Egypt/CL6/07) induced apoptosis in duck embryonic fibroblasts (DEF). In contrast, apoptosis was reduced among cells infected with low-pathogenic AIVs (A/duck/HK/342/78 [H5N2], A/duck/HK/820/80 [H5N3], A/wigeon/Osaka/1/01 [H7N7], and A/turkey/Wisconsin/1/66 [H9N2]). Thus, we investigated the molecular mechanisms of apoptosis induced by H5N1-AIV infection. Caspase-dependent and -independent pathways contributed to the cytopathic effects. We further showed that, in the induction of apoptosis, the hemagglutinin of H5N1-AIV played a major role and its cleavage sequence was not critical. We also observed outer membrane permeabilization and loss of the transmembrane potential of the mitochondria of infected DEF, indicating that mitochondrial dysfunction was caused by the H5N1-AIV infection. We then analyzed Ca2+ dynamics in the infected cells and demonstrated an increase in the concentration of Ca2+ in the cytosol ([Ca2+]i) and mitochondria ([Ca2+]m) after H5N1-AIV infection. Regardless, gene expression important for regulating Ca2+ efflux from the endoplasmic reticulum did not significantly change after H5N1-AIV infection. These results suggest that extracellular Ca2+ may enter H5N1-AIV-infected cells. Indeed, EGTA, which chelates extracellular free Ca2+, significantly reduced the [Ca2+]i, [Ca2+]m, and apoptosis induced by H5N1-AIV infection. In conclusion, we identified a novel mechanism for influenza A virus-mediated cell death, which involved the acceleration of extracellular Ca2+ influx, leading to mitochondrial dysfunction and apoptosis. These findings may be useful for understanding the pathogenesis of H5N1-AIV in avian species as well as the impact of Ca2+ homeostasis on influenza A virus infection.

scholarly journals Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems

2011 ◽  
Vol 8 (1) ◽  
pp. 196 ◽  
Author(s):  
Vinod RMT Balasubramaniam ◽  
Sharifah S Hassan ◽  
Abdul R Omar ◽  
Maizan Mohamed ◽  
Suriani M Noor ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Pathogenic H5n1

siRNAs targeting PB2 and NP genes potentially inhibit replication of Highly Pathogenic H5N1 Avian Influenza Virus

2015 ◽  
Vol 40 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Padmanava Behera ◽  
Shanmugasundaram Nagarajan ◽  
Harshad V Murugkar ◽  
Semmannan Kalaiyarasu ◽  
Anil Prakash ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Pathogenic H5n1
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