Recessive Genic Male Sterility in <italic>Brassica napus</italic> and Its Application

2014 ◽  
Vol 44 (8) ◽  
pp. 752-757
Author(s):  
JinXing TU ◽  
TingDong FU ◽  
Bin YI
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Genic Male Sterility ◽  
Recessive Genic Male Sterility

Fine mapping of the epistatic suppressor gene (esp) of a recessive genic male sterility in rapeseed (Brassica napus L.)

Genome ◽  
2009 ◽  
Vol 52 (9) ◽  
pp. 755-760 ◽  
Author(s):  
Zhenghua Xu ◽  
Yanzhou Xie ◽  
Dengfeng Hong ◽  
Pingwu Liu ◽  
Guangsheng Yang
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Fine Mapping ◽  
Ssr Marker ◽  
Scar Marker ◽  
Spontaneous Mutation ◽  
Suppressor Gene ◽  
Brassica Napus L ◽  
Genic Male Sterility ◽  
Recessive Genic Male Sterility

9012AB, a recessive genic male sterility (RGMS) line derived from spontaneous mutation in Brassica napus , has been playing an important role in rapeseed hybrid production in China. The male sterility of 9012AB is controlled by two recessive genes (ms3 and ms4) interacting with one recessive epistatic suppressor gene (esp). The objective of this study was to develop PCR-based markers tightly linked to the esp gene and construct a high-resolution map surrounding the esp gene. From the survey of 512 AFLP primer combinations, 3 tightly linked AFLP markers were obtained and successfully converted to codominant or dominant SCAR markers. Furthermore, a codominant SSR marker (Ra2G08) associated with the esp gene was identified through genetic map integration. For fine mapping of the esp gene, these PCR-based markers were analyzed in a large BC1 population of 2545 plants. The esp gene was then genetically restricted to a region of 1.03 cM, 0.35 cM from SSR marker Ra2G08 and 0.68 cM from SCAR marker WSC6. The SCAR marker WSC5 co-segregated with the target gene. These results lay a solid foundation for map-based cloning of esp and will facilitate the selection of RGMS lines and their temporary maintainers.

Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L.

2012 ◽  
Vol 30 (2) ◽  
pp. 1193-1205 ◽  
Author(s):  
Faming Dong ◽  
Dengfeng Hong ◽  
Yanzhou Xie ◽  
Yanping Wen ◽  
Li Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Brassica Napus L ◽  
Multiple Allele ◽  
Genic Male Sterility ◽  
Recessive Genic Male Sterility

Fine mapping of the recessive genic male sterility gene (Bnms3) in Brassica napus L.

2007 ◽  
Vol 115 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Zhen Huang ◽  
Yufeng Chen ◽  
Bin Yi ◽  
Lu Xiao ◽  
Chaozhi Ma ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Fine Mapping ◽  
Brassica Napus L ◽  
Genic Male Sterility ◽  
Recessive Genic Male Sterility ◽  
Sterility Gene ◽  
Male Sterility Gene
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