Construction of SSH Library and Resistance Gene Analyses of Yunnan <italic>Oryza rufipogon</italic> Griff. under <italic>Xanthomonas oryzae</italic> pv. <italic>Oryzae</italic> Stress

2013 ◽  
Vol 43 (11) ◽  
pp. 972-980
Author(s):  
ZaiQuan CHENG ◽  
ZhengWen SUN ◽  
QiaoFang ZHONG ◽  
Jian FU ◽  
DingQin LI ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Oryza Rufipogon ◽  
Xanthomonas Oryzae ◽  
Ssh Library

scholarly journals Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
SITI YURIYAH ◽  
DEDY DARNAEDI ◽  
TATANG MITRA SETIA ◽  
GUT WINDARSIH ◽  
Dwinita Wikan Utami
Keyword(s):  
Resistance Gene ◽  
Wild Rice ◽  
Leaf Blight ◽  
Oryza Rufipogon ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Potential ◽  
Interspecific Crosses ◽  
Blight Resistance ◽  
Wild Rice Species

Abstract. Yuriyah S, Darnaedi D, Setia TM, Windarsih G, Utami DW. 2021. Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character. Biodiversitas 22: 4123-4130. Wild rice species are the source of the gene pool for rice genetic diversity. The cross-species crosses (interspecific crosses) play an important role in breeding, namely in terms of expanding the diversity of desirable characters, such as disease resistance and improvement in yield potential. Currently, the genes from wild rice species have been successfully introgressed into cultivated rice so that they can overcome the rice production constraints, including the introgression of genes for the bacterial leaf blight (BLB) resistance. The purpose of this study was to analyze the variability of phenotype and genotype performance of lines derived from wild rice species for the character of resistance to BLB disease. A total of 33 selected backcross lines derived from wild rice species of O. rufipogon and O. glaberrima as the donor parents and 2 control varieties (resistant and susceptible to BLB disease), were used in this study. The evaluation of phenotype and genotype of resistance characters to BLB disease was carried out during a vegetative phase of the plant. The resistance evaluation was carried out in a greenhouse, while the genotype performance was analyzed using 4 STS markers linked to Xa4, Xa7, and Xa13 genes. The result of the phenotype evaluation showed that there were variations among the resistance of the tested lines. Three lines derived from a Situ Bagendit/Oryza rufipogon cross were resistant to all BLB races used in the testing. Those three lines indicated to have the allele of the Xa7 resistance gene based on the genotype performance which grouped with the Code variety that had the Xa7 resistance gene.

Identification and Molecular Mapping of Xa32(t), a Novel Resistance Gene for Bacterial Blight (Xanthomonas oryzae pv. oryzae) in Rice

2009 ◽  
Vol 35 (7) ◽  
pp. 1173-1180 ◽  
Author(s):  
Chong-Ke ZHENG ◽  
Chun-Lian WANG ◽  
Yuan-Jie YU ◽  
Yun-Tao LIANG ◽  
Kai-Jun ZHAO
Keyword(s):  
Resistance Gene ◽  
Bacterial Blight ◽  
Molecular Mapping ◽  
Xanthomonas Oryzae

scholarly journals Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.

2012 ◽  
Vol 11 (6) ◽  
pp. 962-969 ◽  
Author(s):  
Wen-ai HE ◽  
Da-hui HUANG ◽  
Rong-bai LI ◽  
Yong-fu QIU ◽  
Jian-dong SONG ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Bacterial Leaf Streak ◽  
Oryza Rufipogon Griff

scholarly journals High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.)

2018 ◽  
Vol 68 (2) ◽  
pp. 188-199 ◽  
Author(s):  
Constantine Busungu ◽  
Satoru Taura ◽  
Jun-Ichi Sakagami ◽  
Toyoaki Anai ◽  
Katsuyuki Ichitani
Keyword(s):  
Oryza Sativa ◽  
High Resolution ◽  
Resistance Gene ◽  
Xanthomonas Oryzae ◽  
High Resolution Mapping ◽  
Resolution Mapping

Identification of the blast resistance gene Picl(t) from Chaling common wild rice ( Oryza rufipogon Griff.)

2020 ◽  
Vol 168 (4) ◽  
pp. 211-219
Author(s):  
Chuanqin Zheng ◽  
Nan Jiang ◽  
Xinhui Zhao ◽  
Tianze Yan ◽  
Jun Fu ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Wild Rice ◽  
Blast Resistance ◽  
Oryza Rufipogon ◽  
Blast Resistance Gene ◽  
Common Wild Rice ◽  
Oryza Rufipogon Griff

scholarly journals Cloning of the rice Xo1 resistance gene and interaction of the Xo1 protein with the defense-suppressing Xanthomonas effector Tal2h

2020 ◽  
Author(s):  
Andrew C. Read ◽  
Mathilde Hutin ◽  
Matthew J. Moscou ◽  
Fabio C. Rinaldi ◽  
Adam J. Bogdanove
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Bacterial Blight ◽  
Rice Variety ◽  
Susceptible Variety ◽  
Xanthomonas Oryzae ◽  
Physical Interaction ◽  
Bacterial Leaf Streak ◽  
Transcription Activator ◽  
Rice Leaves

AbstractThe Xo1 locus in the heirloom rice variety Carolina Gold Select confers resistance to bacterial leaf streak and bacterial blight, caused by Xanthomonas oryzae pvs. oryzicola and oryzae, respectively. Resistance is triggered by pathogen-delivered transcription activator-like effectors (TALEs) independent of their ability to activate transcription, and is suppressed by variants called truncTALEs common among Asian strains. By transformation of the susceptible variety Nipponbare, we show that one of 14 nucleotide-binding, leucine-rich repeat (NLR) protein genes at the locus, with a zfBED domain, is the Xo1 gene. Analyses of published transcriptomes revealed that the Xo1-mediated response is similar to those of NLR resistance genes Pia and Rxo1 and distinct from that associated with induction of the executor resistance gene Xa23, and that a truncTALE dampens or abolishes activation of defense-associated genes by Xo1. In Nicotiana benthamiana leaves, fluorescently-tagged Xo1 protein, like TALEs and truncTALEs, localized to the nucleus. And, endogenous Xo1 specifically co-immunoprecipitated from rice leaves with a pathogen-delivered, epitope-tagged truncTALE. These observations suggest that suppression of Xo1-function by truncTALEs occurs through direct or indirect physical interaction. They further suggest that effector co-immunoprecipitation may be effective for identifying or characterizing other resistance genes.

scholarly journals Identification of a novel planthopper resistance gene from wild rice (Oryza rufipogon Griff.)

2020 ◽  
Vol 8 (6) ◽  
pp. 1057-1070
Author(s):  
Meng Yang ◽  
Jiebin Lin ◽  
Ling Cheng ◽  
Hailian Zhou ◽  
Shu Chen ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Oryza Rufipogon Griff

scholarly journals Identification and Fine-Mapping of Xa33, a Novel Gene for Resistance to Xanthomonas oryzae pv. oryzae

2012 ◽  
Vol 102 (2) ◽  
pp. 222-228 ◽  
Author(s):  
P. Natraj Kumar ◽  
K. Sujatha ◽  
G. S. Laha ◽  
K. Srinivasa Rao ◽  
B. Mishra ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Resistance Gene ◽  
Ssr Markers ◽  
Rice Genome ◽  
Dominant Gene ◽  
Xanthomonas Oryzae ◽  
Chromosome 7 ◽  
Progeny Testing ◽  
Putative Gene ◽  
The Cross

Broadening of the genetic base for identification and transfer of genes for resistance to insect pests and diseases from wild relatives of rice is an important strategy in resistance breeding programs across the world. An accession of Oryza nivara, International Rice Germplasm Collection (IRGC) accession number 105710, was identified to exhibit high level and broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. In order to study the genetics of resistance and to tag and map the resistance gene or genes present in IRGC 105710, it was crossed with the bacterial blight (BB)-susceptible varieties ‘TN1’ and ‘Samba Mahsuri’ (SM) and then backcrossed to generate backcross mapping populations. Analysis of these populations and their progeny testing revealed that a single dominant gene controls resistance in IRGC 105710. The BC1F2 population derived from the cross IRGC 105710/TN1//TN1 was screened with a set of 72 polymorphic simple-sequence repeat (SSR) markers distributed across the rice genome and the resistance gene was coarse mapped on chromosome 7 between the SSR markers RM5711 and RM6728 at a genetic distance of 17.0 and 19.3 centimorgans (cM), respectively. After analysis involving 49 SSR markers located between the genomic interval spanned by RM5711 and RM6728, and BC2F2 population consisting of 2,011 individuals derived from the cross IRGC 105710/TN1//TN1, the gene was fine mapped between two SSR markers (RMWR7.1 and RMWR7.6) located at a genetic distance of 0.9 and 1.2 cM, respectively, from the gene and flanking it. The linkage distances were validated in a BC1F2 mapping population derived from the cross IRGC 105710/SM//2 × SM. The BB resistance gene present in the O. nivara accession was identified to be novel based on its unique map location on chromosome 7 and wider spectrum of BB resistance; this gene has been named Xa33. The genomic region between the two closely flanking SSR markers was in silico analyzed for putatively expressed candidate genes. In total, eight genes were identified in the region and a putative gene encoding serinethreonine kinase appears to be a candidate for the Xa33 gene.

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