Study on fibroin heavy chain of the silkworm Bombyx mori by fluorescence in situ hybridization (FISH)

2002 ◽  
Vol 45 (6) ◽  
pp. 663 ◽  
Author(s):  
Fangzhou SONG
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Bombyx Mori ◽  
Heavy Chain ◽  
Silkworm Bombyx Mori

Waldenström macroglobulinemia neoplastic cells lack immunoglobulin heavy chain locus translocations but have frequent 6q deletions

Blood ◽  
2002 ◽  
Vol 100 (8) ◽  
pp. 2996-3001 ◽  
Author(s):  
Roelandt F. J. Schop ◽  
W. Michael Kuehl ◽  
Scott A. Van Wier ◽  
Gregory J. Ahmann ◽  
Tammy Price-Troska ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Cells ◽  
Southern Blot ◽  
Heavy Chain ◽  
Genomic Stability ◽  
Immunoglobulin M ◽  
Lymphoplasmacytic Lymphoma ◽  
Interphase Fish

Lymphoplasmacytic lymphoma (LPL) is characterized by t(9;14)(p13;q32) in 50% of patients who lack paraproteinemia. Waldenström macroglobulinemia (WM), which has an immunoglobulin M (IgM) paraproteinemia, is classified as an LPL. Rare reports have suggested that WM sometimes is associated with 14q23 translocations, deletions of 6q, and t(11;18)(q21;q21). We tested for these abnormalities in the clonal cells of WM patients. We selected patients with clinicopathologic diagnosis of WM (all had IgM levels greater than 1.5 g/dL). Southern blot assay was used to detect legitimate and illegitimate IgH switch rearrangements. In addition to conventional cytogenetic (CC) and multicolor metaphase fluorescence in situ hybridization (M-FISH) analyses, we used interphase FISH to screen for t(9;14)(p13;q32) and other IgH translocations, t(11;18)(q21;q21), and 6q21 deletions. Genomic stability was also assessed using chromosome enumeration probes for chromosomes 7, 9, 11, 12, 15, and 17 in 15 patients. There was no evidence of either legitimate or illegitimate IgH rearrangements by Southern blot assay (n = 12). CC (n = 37), M-FISH (n = 5), and interphase FISH (n = 42) failed to identify IgH or t(11;18) translocations. Although tumor cells from most patients were diploid for the chromosomes studied, deletions of 6q21 were observed in 42% of patients. In contrast to LPL tumors that are not associated with paraproteinemia and that have frequent t(9;14)(p13;q32) translocations, IgH translocations are not found in WM, a form of LPL tumor distinguished by IgM paraproteinemia. However, WM tumor cells, which appear to be diploid or near diploid, often have deletions of 6q21.

scholarly journals Determining Gene Location on Chromosomes of Bombyx mori Using Fluorescence in situ Hybridization (FISH)

1995 ◽  
Vol 30 (1) ◽  
pp. 225-230 ◽  
Author(s):  
Kozo TSUCHIDA ◽  
Naoko MIYAJIMA ◽  
Yosuke KAMEOKA ◽  
Naoko TAKADA ◽  
Hideaki MAEKAWA
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Bombyx Mori ◽  
Gene Location

scholarly journals Aberrations of the immunoglobulin heavy chain locus in patients with chronic lymphocytic leukemia detected with fluorescence in situ hybridization

2019 ◽  
pp. 57-64
Author(s):  
Т.Г. Шкаврова ◽  
Г.Ф. Михайлова ◽  
В.В. Цепенко ◽  
Е.В. Голуб
Keyword(s):  
In Situ Hybridization ◽  
Chronic Lymphocytic Leukemia ◽  
Fluorescence In Situ Hybridization ◽  
Heavy Chain ◽  
Clinical Course ◽  
Lymphocytic Leukemia ◽  
Favorable Prognosis ◽  
Igh Locus ◽  
Cytogenetic Aberrations

Актуальность. К настоящему времени известно, что в патогенезе хронического лимфоцитарного лейкоза (ХЛЛ) важную роль играют генетические нарушения. Их выявление может быть использовано для оценки прогноза заболевания, что является актуальной задачей, поскольку по клиническому течению ХЛЛ крайне разнороден. Цель исследования. Изучить молекулярно-цитогенетические нарушения в локусе IGH у больных ХЛЛ и их сочетание с нарушениями, выявленными при использовании стандартной для ХЛЛ цитогенетической панели. Материалы и методы. Исследование выполнено методом интерфазной in situ гибридизации (I-FISH) на архивных образцах нестимулированных лимфоцитов 89 больных с впервые выявленным ХЛЛ и 15 клинически здоровых доноров в качестве контрольной группы. Результаты. Нарушения в локусе тяжелых цепей иммуноглобулинов обнаружены у 64% больных. IGH транслокации выявлены у 19% больных, частичная/полная потеря одной копии IGHV региона - у 58% больных ХЛЛ. Мы разделили больных на 3 группы и оценили встречаемость в них нарушений в локусе 14q32. В группу неблагоприятного прогноза вошли 35 человек (16 больных с del17p13 и 19 больных с del11q22), в группу промежуточного прогноза вошли 18 человек (10 больных с трисомией хромосомы 12 и 8 больных без выявленных цитогенетических нарушений, и в группу благоприятного прогноза - 36 больных с del13q14 как единственной. Статистически значимых различий между группами прогноза по встречаемости больных с IGH транслокациями не выявлено. Установлено, что у больных с del17p13 и с del11q22 IGHV делеции встречались достоверно чаще, чем у больных с del13q14 как единственной (74% vs 44%, p = 0,011). В группах неблагоприятного и промежуточного прогноза по сравнению с группой благоприятного прогноза почти в 4 раза (40% и 39% vs 11% соответственно) больше больных с высокой (50% и выше) долей клеток с IGHV делециями (p < 0,05). Заключение. Нарушения в IGH локусе занимают второе место по встречаемости среди нарушений, выявляемых методом FISH при ХЛЛ. Эти нарушения могут быть значимыми, поскольку они достоверно чаще встречаются у больных в группе неблагоприятного прогноза. Дальнейшее наблюдение за больными c нарушениями в IGH локусе позволит оценить их клинико-биологические особенности и прогностическую значимость при ХЛЛ. Возможно, гетерогенность течения заболевания внутри группы больных с благоприятным прогнозом, выделенной на основании иерархической модели, обусловлена наличием дополнительных цитогенетических нарушений, в том числе и нарушений в IGH локусе. Background. Genetic disorders play an important role in the pathogenesis of chronic lymphocytic leukemia (CLL). Identification of these disorders can be used for prognosis of the disease. This is an urgent task since the clinical course of CLL is extremely heterogeneous. The aim of this study was to explore cytogenetic aberrations of the immunoglobulin heavy chain (IGH) locus and their association with a common FISH panel in patients with chronic lymphocytic leukemia (CLL). Materials and methods. The study was performed by a retrospective analysis of samples of non-stimulated lymphocytes from 89 patients with newly diagnosed CLL and 15 clinically healthy donors as a control group using interphase fluorescence in situ hybridization (I-FISH). Results. Rearrangements of the IGH locus were found in 64% of CLL patients. IGH translocations were observed in 19% of patients whereas 58% of patients had total or partial IGHV deletions. The patients were divided into three groups and evaluated for occurrence of aberrations in the locus 14q32. The group of unfavorable prognosis included 35 patients (16 patients with del17p13 and 19 patients with del11q22); the group of intermediate prognosis included 18 patients (10 patients with chromosome 12 trisomy and 8 patients without identified aberrations); and the group of favorable prognosis consisted of 36 patients with del13q14 as the sole abnormality. There were no statistically significant differences between groups with different prognosis in the occurrence of patients with IGH translocations. IGHV deletions were significantly more frequent in patients with del17p13 and del11q22 than in patients with del13q14 as the sole abnormality (74% vs. 44%, p=0.011). Patients with a high (50% and more) proportion of cells with IGHV deletions occurred approximately 4 times more frequently in groups of unfavorable and intermediate prognosis compared to the group of favorable prognosis (40% and 39% vs. 11%, respectively, p<0.05). Conclusion. Rearrangements of the IGH locus detected using the FISH method is the second among all aberrations in CLL. These abnormalities may be important as they are significantly more frequently observed in patients with an unfavorable prognosis. Further observation of CLL patients with IGH aberrations will allow to identify clinical and biological features and prognostic importance of these aberrations. Probably, the intergroup heterogeneity of the clinical course in patients with a favorable prognosis determined on a hierarchical model is due to additional cytogenetic aberrations including IGH locus abnormalities.

Localization of the Human Cytoplasmic Dynein Heavy Chain (DNECL) to 14qter by Fluorescence in Situ Hybridization

Genomics ◽  
1994 ◽  
Vol 22 (3) ◽  
pp. 660-661 ◽  
Author(s):  
D. Narayan ◽  
T. Desai ◽  
A. Banks ◽  
S.R. Patanjali ◽  
T.S. Ravikumar ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Heavy Chain ◽  
Cytoplasmic Dynein ◽  
Dynein Heavy Chain
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