Genome organization and variation in the 3?-partial sequence of garlic latent virus in China

2002 ◽  
Vol 45 (4) ◽  
pp. 441 ◽  
Author(s):  
Jiong CHEN
Keyword(s):  
Genome Organization ◽  
Latent Virus ◽  
Partial Sequence ◽  
Garlic Latent Virus

scholarly journals First Report of Shallot latent virus in Garlic in Argentina

Plant Disease ◽  
2010 ◽  
Vol 94 (7) ◽  
pp. 915-915 ◽  
Author(s):  
A. K. Torrico ◽  
E. E. Cafrune ◽  
V. C. Conci
Keyword(s):  
Latent Virus ◽  
Onion Yellow Dwarf Virus ◽  
Rt Pcr ◽  
Cp Gene ◽  
Virus Complex ◽  
Garlic Latent Virus ◽  
Shallot Latent Virus ◽  
Yellow Dwarf Virus ◽  
Garlic Virus ◽  
Das Elisa

Because of exclusively agamic propagation, garlic is commonly infected with a virus complex mainly composed of species within the genera Potyvirus, Allexivirus, and Carlavirus. This virus complex causes leaf striping that ranges from various shades of green to yellow and results in yield losses (2,4). Onion yellow dwarf virus, Leek yellow stripe virus (potyviruses), Garlic virus A, Garlic virus C (allexiviruses), and Garlic common latent virus (carlavirus) have been detected in Argentina previously (1,2). Recently, Shallot latent virus (SLV; another carlavirus) was detected in 25 of 30 garlic plants (cv. Morado) growing in four different fields near Córdoba, Argentina by double-antibody sandwich (DAS)-ELISA using BIOREBA (Reinach, Switzerland) antibodies. To confirm the presence of the virus, DAS-ELISA-positive plants were also analyzed by one-step reverse transcription (RT)-PCR using the Access RT-PCR system (Promega, Madison, WI) with specific primers reported by Tsuneyoshi et al. (3). RNA extractions were performed from 100 mg of leaves with the Qiagen RNeasy Plant Mini Kit (Qiagen, Valencia, CA). Primers used were Car-V1 (5′-AAACCTTTTGGTTCACTTTAGG-3′); Car-V2 (5′-AGGTGCATTGTTATCATTACTGG-3′); and Car-Cp3 (5′-GCGTGCTATATTTAAGTTGCATAC-3′). Primer pairs Car-V1/Car-Cp3 and Car-V2/Car-Cp3 were used for the amplification of the coat protein (CP) gene of SLV and an isolate of SLV formerly known as Garlic latent virus, respectively. Fragments of 992 bp and 1,079 bp were amplified with these primer pairs, respectively. The RT-PCR products were cloned with the TOPO TA Cloning Kit in the 3.9-kb pCR-TOPO vector (Qiagen). The nucleotide sequences of both fragments were determined and were found to be identical (GenBank No. GU355922) showing 94.2% nt sequence identity with the CP gene of an isolate of SLV from Indonesian garlic (GenBank No. AB004686) formerly referred to as Garlic latent virus (3). Consequently, the Argentinean virus is now considered a garlic isolate of SLV. References: (1) E. Cafrune et al. Plant Dis. 90:898, 2006. (2) V. C. Conci. Virus y Fitoplasmas de Ajo. Page 267 in: 50 Temas Sobre Producción de Ajo. Vol. 3. J. L. Burba, ed. Ediciones INTA, Mendoza, Argentina. 1997. (3) T. Tsuneyoshi et al. Arch. Virol. 143:1093, 1998. (4) D. G. A. Walkey and D. N. Antill. J. Hortic. Sci. 64:53, 1989.

scholarly journals Simple and rapid detection of garlic latent virus from Welsh onions by gelatin particle agglutination test.

1989 ◽  
Vol 55 (5) ◽  
pp. 671-675 ◽  
Author(s):  
Masanobu FUKAMI ◽  
Keiko T. NATSUAKI ◽  
Fusao MOTOYOSHI ◽  
Keiichi TOMARU
Keyword(s):  
Rapid Detection ◽  
Agglutination Test ◽  
Latent Virus ◽  
Garlic Latent Virus ◽  
Particle Agglutination

scholarly journals Transmission and translocation of garlic latent virus in rakkyo (A Ilium Chinense G. Don)

1990 ◽  
Vol 32 (0) ◽  
pp. 21-27
Author(s):  
Isamu SAKO ◽  
Tatsuo TANIGUCHI ◽  
Takeshi OSAKI ◽  
Tadao INOUYE
Keyword(s):  
Latent Virus ◽  
Garlic Latent Virus

scholarly journals Yellow streak of rakkyo(Allium chinense G.Don), a newly recognized disease caused by garlic latent virus and onion yellow dwarf virus.

1991 ◽  
Vol 57 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Isamu SAKO ◽  
Wataru NAKASONE ◽  
Kiyotugu OKADA ◽  
Satoshi T. OHKI ◽  
Takeshi OSAKI ◽  
...  
Keyword(s):  
Latent Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Garlic Latent Virus ◽  
Yellow Dwarf Virus ◽  
Allium Chinense

Complete nucleotide sequence and genome organization of Olive latent virus 3, a new putative member of the family Tymoviridae

2010 ◽  
Vol 152 (1-2) ◽  
pp. 10-18 ◽  
Author(s):  
Abdulkader Alabdullah ◽  
Angelantonio Minafra ◽  
Toufic Elbeaino ◽  
Maria Saponari ◽  
Vito Savino ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genome Organization ◽  
Complete Nucleotide Sequence ◽  
Latent Virus ◽  
The Family ◽  
Putative Member

scholarly journals Occurrence and incidence of Garlic Latent Virus in Rakkyo (Allium chinense G. Don)

1989 ◽  
Vol 31 (0) ◽  
pp. 23-29
Author(s):  
Isamu SAKO ◽  
Wataru NAKASONE ◽  
Kiyotugu OKADA ◽  
Takeshi OSAKI ◽  
Tadao INOUYE
Keyword(s):  
Latent Virus ◽  
Garlic Latent Virus ◽  
Allium Chinense
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