Cloning of the promoter region of plasma membrane aquaporin BnPIP1 from Brassica napus and its functional analysis

2003 ◽  
Vol 46 (4) ◽  
pp. 348 ◽  
Author(s):  
Qiuju YU
Keyword(s):  
Functional Analysis ◽  
Plasma Membrane ◽  
Brassica Napus ◽  
Promoter Region

Isolation of the promoter region of HAK gene from Aeluropus littoralis and functional analysis in rice

2012 ◽  
Vol 34 (6) ◽  
pp. 742-748 ◽  
Author(s):  
Gao-Hua ZHANG ◽  
He WANG ◽  
Xu-Da WANG ◽  
Ming FENG ◽  
Huai-Mei LI ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Promoter Region ◽  
Aeluropus Littoralis ◽  
I Gene

Functional Analysis of BnFAD2-C5 Promoter and Intron at Expression Level in Brassica napus

2016 ◽  
Vol 42 (10) ◽  
pp. 1471 ◽  
Author(s):  
Rui-Yang LIU ◽  
Fang LIU ◽  
Zhen-Qian ZHANG ◽  
Chun-Yun GUAN
Keyword(s):  
Functional Analysis ◽  
Brassica Napus ◽  
Expression Level

scholarly journals Evolutionary and Functional Analysis of a Chara Plasma Membrane H+-ATPase

2020 ◽  
Vol 10 ◽  
Author(s):  
Suyun Zhang ◽  
Myckel Habets ◽  
Holger Breuninger ◽  
Liam Dolan ◽  
Remko Offringa ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Plasma Membrane

The Candida albicans plasma membrane protein Rch1p, a member of the vertebrate SLC10 carrier family, is a novel regulator of cytosolic Ca2+ homoeostasis

2012 ◽  
Vol 444 (3) ◽  
pp. 497-502 ◽  
Author(s):  
Linghuo Jiang ◽  
Joerg Alber ◽  
Jihong Wang ◽  
Wei Du ◽  
Xuexue Yang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Candida Albicans ◽  
Plasma Membrane ◽  
Membrane Protein ◽  
Solute Carrier Family ◽  
Plasma Membrane Protein ◽  
Cellular Functions ◽  
Downstream Target ◽  
Solute Carrier ◽  
High Concentrations

Candida albicans RCH1 (regulator of Ca2+ homoeostasis 1) encodes a protein of ten TM (transmembrane) domains, homologous with human SLC10A7 (solute carrier family 10 member 7), and Rch1p localizes in the plasma membrane. Deletion of RCH1 confers hypersensitivity to high concentrations of extracellular Ca2+ and tolerance to azoles and Li+, which phenocopies the deletion of CaPMC1 (C. albicans PMC1) encoding the vacuolar Ca2+ pump. Additive to CaPMC1 mutation, lack of RCH1 alone shows an increase in Ca2+ sensitivity, Ca2+ uptake and cytosolic Ca2+ level. The Ca2+ hypersensitivity is abolished by cyclosporin A and magnesium. In addition, deletion of RCH1 elevates the expression of CaUTR2 (C. albicans UTR2), a downstream target of the Ca2+/calcineurin signalling. Mutational and functional analysis indicates that the Rch1p TM8 domain, but not the TM9 and TM10 domains, are required for its protein stability, cellular functions and subcellular localization. Therefore Rch1p is a novel regulator of cytosolic Ca2+ homoeostasis, which expands the functional spectrum of the vertebrate SLC10 family.

Bioinformatic and functional analysis of promoter region of human SLC25A13 gene

Gene ◽  
2019 ◽  
Vol 693 ◽  
pp. 69-75 ◽  
Author(s):  
Jun-Lin Chen ◽  
Zhan-Hui Zhang ◽  
Bing-Xiao Li ◽  
Zhen Cai ◽  
Qing-Hua Zhou
Keyword(s):  
Functional Analysis ◽  
Promoter Region

Functional analysis of the promoter region of amphioxus β-actin gene: a useful tool for driving gene expression in vivo

2014 ◽  
Vol 41 (10) ◽  
pp. 6817-6826 ◽  
Author(s):  
Jun Feng ◽  
Guang Li ◽  
Xin Liu ◽  
Jing Wang ◽  
Yi-Quan Wang
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Promoter Region ◽  
Actin Gene

124 Functional analysis of porcine OCT4 transcriptional regulatory region-based reporter system

2019 ◽  
Vol 31 (1) ◽  
pp. 187
Author(s):  
S.-H. Kim ◽  
K.-H. Choi ◽  
D.-K. Lee ◽  
M. Lee ◽  
M.-H. Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Analysis ◽  
Embryonic Stem Cells ◽  
Promoter Region ◽  
Fluorescent Protein ◽  
Regulatory Region ◽  
Embryonic Stem ◽  
Upstream Region ◽  
Reporter Assay ◽  
Reporter System

Gene OCT4 plays pivotal roles in maintaining pluripotency of early mammalian embryonic development and embryonic stem cells. It is essential to establish a reporter system based on the OCT4 promoter region for the study of pluripotency. However, there is still a lack of sufficient information about the porcine OCT4 upstream reporter system. To improve our understanding of the porcine OCT4 regulatory region, first, we conducted an investigation to find conserved regions in the porcine OCT4 promoter upstream region by sequence-based comparative analysis using various mammalian genome sequences. A similarity of nucleotide sequences of the 5′ upstream region was low among mammalian species. However, the OCT4 promoter and 4 regulatory regions including distal and proximal enhancer elements have a high similarity. Next, a functional analysis of the porcine OCT4 promoter region was conducted. Luciferase reporter assay indicated that the porcine OCT4 distal enhancer and proximal enhancer are highly activated in mouse embryonic stem cells and embryonic carcinoma cells, respectively (n=3). Comparison analysis of naïve (Tbx3, Nr0b1, Rex1, Esrrb, Nanog, Klf2) or primed (Gata6, Mixl1, Fgf5, Otx2) state marker gene expression in a dual-reporter assay using pOCT4-DE-eGFP and pOCT4-PE-DsRed2 showed that expression of naïve and primed markers were up-regulated in cells with high green fluorescent protein and red fluorescent protein expression, respectively (n=3). Porcine OCT4-upstream region-based reporter constructs showed exclusive expression patterns depending on the state of pluripotency. This work could provide basic information for the porcine OCT4 upstream region and the various porcine OCT4-fluorescence reporter constructs, which can be applied to study species-specific pluripotency in early embryo development and for the establishment of embryonic stem cells in pigs. This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Development of High Value-Added Food Technology Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA, 118042-03-1-HD020).

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