Subcommittee on Calibration and Test Materials. Recommended Reference Materials for Realization of Physicochemical Properties

1978 ◽  
Vol 50 (11-12) ◽  
pp. 1485-1517 ◽  
Keyword(s):  
Physicochemical Properties ◽  
Reference Materials ◽  
Test Materials

scholarly journals Metabolomics Test Materials for Quality Control: A Study of a Urine Materials Suite

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 270 ◽  
Author(s):  
Daniel W. Bearden ◽  
David A. Sheen ◽  
Yamil Simón-Manso ◽  
Bruce A. Benner ◽  
Werickson F. C. Rocha ◽  
...  
Keyword(s):  
Reference Materials ◽  
Lead Time ◽  
Measurement Techniques ◽  
Chemometric Methods ◽  
Liquid Chromatography Mass Spectrometry ◽  
Gas And Liquid Chromatography ◽  
Metabolomic Data ◽  
Test Materials ◽  
Human Fluid ◽  
Experimental Reference

There is a lack of experimental reference materials and standards for metabolomics measurements, such as urine, plasma, and other human fluid samples. Reasons include difficulties with supply, distribution, and dissemination of information about the materials. Additionally, there is a long lead time because reference materials need their compositions to be fully characterized with uncertainty, a labor-intensive process for material containing thousands of relevant compounds. Furthermore, data analysis can be hampered by different methods using different software by different vendors. In this work, we propose an alternative implementation of reference materials. Instead of characterizing biological materials based on their composition, we propose using untargeted metabolomic data such as nuclear magnetic resonance (NMR) or gas and liquid chromatography-mass spectrometry (GC-MS and LC-MS) profiles. The profiles are then distributed with the material accompanying the certificate, so that researchers can compare their own metabolomic measurements with the reference profiles. To demonstrate this approach, we conducted an interlaboratory study (ILS) in which seven National Institute of Standards and Technology (NIST) urine Standard Reference Material®s (SRM®s) were distributed to participants, who then returned the metabolomic data to us. We then implemented chemometric methods to analyze the data together to estimate the uncertainties in the current measurement techniques. The participants identified similar patterns in the profiles that distinguished the seven samples. Even when the number of spectral features is substantially different between platforms, a collective analysis still shows significant overlap that allows reliable comparison between participants. Our results show that a urine suite such as that used in this ILS could be employed for testing and harmonization among different platforms. A limited quantity of test materials will be made available for researchers who are willing to repeat the protocols presented here and contribute their data.

Role of reference materials for the realization of physicochemical properties. Past, present, and future

2000 ◽  
Vol 72 (10) ◽  
pp. 1809-1818 ◽  
Author(s):  
Kenneth N. Marsh
Keyword(s):  
Physicochemical Properties ◽  
Reference Materials ◽  
State Of The Art ◽  
National Standards ◽  
Correct Operation ◽  
Many Instruments ◽  
Ready Availability ◽  
Commercial Sources

Reference materials have long been considered essential for both calibration and checking the correct operation of equipment used for the determination of physicochemical properties. Up until the 1970s, the maintenance of pure reference materials and the means to verify their properties through measurement using state-of-the-art equipment was the prerogative of many national standards laboratories. Over the last 30 years, many of the standards laboratories have either limited or eliminated their activity in maintaining both reference materials and state-of-the-art measuring equipment. One reason is the ready availability of high-purity materials from commercial sources and the ease with which one can now determine purity. A second reason is the ready availability of instruments to measure digitally fundamental quantities such as time, temperature, length, frequency, and voltage accurately has enabled chemical calibration using reference materials to be replaced in many instruments by electrical calibration. At the same time, digitization has enabled the computerization of the whole measurement process. Such automated devices, if not checked with reference materials, can give highly reproducible results but have large systematic errors, leading to poor values. The role of physicochemical reference materials in the past and the present will be outlined, and their status in the future will be explored.

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