Calorimetric Sensor for Ethanol Using Ni2+-nitrilotriacetic Acid (NTA) Resin Immobilized Alcohol Dehydrogenase (ADH)

2020 ◽  
Vol 16 (6) ◽  
pp. 795-799
Author(s):  
YongJin Li
Keyword(s):  
Cost Effective ◽  
Nitrilotriacetic Acid ◽  
Repeated Measurements ◽  
Great Promise ◽  
Calorimetric Sensor ◽  
Controlled Pore Glass ◽  
Pore Glass ◽  
One Step ◽  
Immobilization Matrix ◽  
Enzyme Thermistor

Background: A simple, fast and economic analytical method for the determination of ethanol is important for clinical, biological, forensic and physico-legal purposes. Methods: Ni2+-NTA resin was used as an immobilization matrix for the simple one-step purification/ immobilization of his6-tagged ADH. Different alcohols with a concentration range of 0.5-50% V/V, namely methanol, ethanol and propanol were measured using prepared ADH enzyme thermistor. The ethanol content of Tsingtao beer was tested as a real sample containing alcohol. Reproducibility and stability of prepared ADH enzyme thermistor were also investigated by repeated measurements. Results: In comparison to the controlled pore glass (a common used support for the immobilization of enzyme) used in thermal biosensor, the use of Ni2+-NTA resin not only led to simple one-step purification/ immobilization by his6-tagged ADH binding to Ni2+-NTA resin, but also made the immobilizing supports reusable. The prepared biosensor can be used to determine ethanol and methanol by the calorimetric measurement. A linear range of 1 -32% (V/V) and 2-20% (V/V) was observed for ethanol and methanol, respectively. The detection limits were 0.3% (V/V) and 1% (V/V) for ethanol and methanol, respectively. The tested ethanol concentration of Tsingtao beer was 4.5% V/V, which is comparable with the labeled alcohol by volume (ABV) 4.80%. Conclusion: Ni2+-NTA resin, as an immobilization matrix in ET sensor, provides a simple one-step purification/immobilization for His6-tagged recombinase and a reusable immobilization matrix. The prepared biosensor exhibits good repeatability and stability. Such a new biosensor shows great promise for rapid, simple, and cost-effective analysis of ethanol and methanol, both in qualitative and in quantitative tests.

Flow Injection Analysis Biosensor for Urea Analysis in Urine Using Enzyme Thermistor

2014 ◽  
Vol 174 (3) ◽  
pp. 998-1009 ◽  
Author(s):  
Geetesh K. Mishra ◽  
Atul Sharma ◽  
Kanchanmala Deshpande ◽  
Sunil Bhand
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Enzyme Thermistor

Biosensing of cholesterol with use of a split-flow enzyme thermistor

1991 ◽  
Vol 5 (1-4) ◽  
pp. 249-252 ◽  
Author(s):  
Ikuo Satoh ◽  
Norihisa Teramura
Keyword(s):  
Split Flow ◽  
Enzyme Thermistor

scholarly journals Enzyme Thermistor Control of the Sucrose Concentration at a Fermentation with Immobilized Yeast.

1980 ◽  
Vol 34b ◽  
pp. 463-465 ◽  
Author(s):  
Carl Fredrik Mandenius ◽  
Bengt Danielsson ◽  
Bo Mattiasson ◽  
Christer von Bahr ◽  
Hans Glaumann
Keyword(s):  
Sucrose Concentration ◽  
Immobilized Yeast ◽  
Enzyme Thermistor

Enzyme thermistor determination of glucose in serum using immobilized glucose oxidase

1977 ◽  
Vol 81 (2) ◽  
pp. 163-175 ◽  
Author(s):  
B. Danielsson ◽  
Kerstin Gadd ◽  
B. Mattiasson ◽  
K. Mosbach
Keyword(s):  
Glucose Oxidase ◽  
Immobilized Glucose Oxidase ◽  
Enzyme Thermistor

Thermal bioanalyzers in flow streams. Enzyme thermistor devices

1981 ◽  
Vol 53 (1) ◽  
pp. 83-94 ◽  
Author(s):  
Klaus. Mosbach ◽  
Bengt. Danielsson
Keyword(s):  
Enzyme Thermistor

scholarly journals Enzyme thermistor analysis of heavy metal ions with use of immobilized urease

FEBS Letters ◽  
1978 ◽  
Vol 85 (2) ◽  
pp. 203-206 ◽  
Author(s):  
Bo Mattiasson ◽  
Bengt Danielsson ◽  
Christer Hermansson ◽  
Klaus Mosbach
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Enzyme Thermistor

Screening of Concanavalin A-Bead Cellulose Conjugates by an Enzyme Thermistor Using Immobilized Invertase as the Reporter Catalyst

1995 ◽  
Vol 750 (1 Enzyme Engine) ◽  
pp. 441-443 ◽  
Author(s):  
PETER DOČOLOMANSKÝ ◽  
PETER GEMEINER ◽  
DANICA MISLOVIČOVÁ ◽  
VLADIMÍR ŠTEFUCA ◽  
BENGT DANIELSSON
Keyword(s):  
Concanavalin A ◽  
Bead Cellulose ◽  
Enzyme Thermistor
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