Dose dependent effects of sodium arsenite on NF-?B and interleukin-8 in bronchial epithelial cells.

2000 ◽  
Author(s):  
Robert R. Roussel
Keyword(s):  
Epithelial Cells ◽  
Sodium Arsenite ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Bronchial Epithelial ◽  
Dose Dependent

scholarly journals Involvement of phospholipases D1 and D2 in sphingosine 1-phosphate-induced ERK (extracellular-signal-regulated kinase) activation and interleukin-8 secretion in human bronchial epithelial cells

2002 ◽  
Vol 367 (3) ◽  
pp. 751-760 ◽  
Author(s):  
Lixin WANG ◽  
Rhett CUMMINGS ◽  
Peter USATYUK ◽  
Andrew MORRIS ◽  
Kaikobad IRANI ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Wild Type ◽  
Bronchial Epithelial ◽  
Extracellular Signal Regulated Kinase ◽  
Erk Phosphorylation ◽  
Extracellular Signal ◽  
Sphingosine 1 Phosphate ◽  
Dose Dependent

Sphingosine 1-phosphate (S1P), a metabolite of sphingomyelin degradation, stimulates interleukin-8 (IL-8) secretion in human bronchial epithelial (Beas-2B) cells. The molecular mechanisms regulating S1P-mediated IL-8 secretion are yet to be completely defined. Here we provide evidence that activation of phospholipases D1 and D2 (PLD1 and PLD2) by S1P regulates the phosphorylation of extracellular-signal-regulated kinase (ERK) and IL-8 secretion in Beas-2B cells. S1P, in a time- and dose-dependent manner, enhanced the threonine/tyrosine phosphorylation of ERK. The inhibition of S1P-induced ERK phosphorylation by pertussis toxin and PD 98059 indicated coupling of S1P receptors to Gi and the ERK signalling cascade respectively. Treatment of Beas-2B cells with butan-1-ol, but not butan-3-ol, abrogated the S1P-induced phosphorylation of Raf-1 and ERK, suggesting that PLD is involved in this activation. The roles of PLD1 and PLD2 in ERK activation and IL-8 secretion activated by S1P were investigated by infecting cells with adenoviral constructs of wild-type and catalytically inactive mutants of PLD1 and PLD2. Infection of Beas-2B cells with the wild-type constructs resulted in the activation of PLD1 and PLD2 by S1P and PMA. Also, the enhanced production of [32P]phosphatidic acid and [32P]phosphatidylbutanol in the presence of butan-1-ol and the increased phosphorylation of ERK by S1P were blocked by the catalytically inactive mutants hPLD1-K898R and mPLD2-K758R. Transient transfection of Beas-2B cells with human PLD1 and mouse PLD2 cDNAs potentiated S1P-mediated IL-8 secretion compared with vector controls. In addition, PD 98059 attenuated IL-8 secretion induced by S1P in a dose-dependent fashion. These results demonstrate that both PLD1 and PLD2 participate in S1P stimulation of ERK phosphorylation and IL-8 secretion in bronchial epithelial cells.

N-acetyl- L-cysteine inhibits bleomycin-induced interleukin-8 secretion by bronchial epithelial cells

Respirology ◽  
2000 ◽  
Vol 5 (4) ◽  
pp. 309-313 ◽  
Author(s):  
Yasuhiro Gon ◽  
Shu Hashimoto ◽  
Tomoko Nakayama ◽  
Ken Matsumoto ◽  
Toshiya Koura ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Bronchial Epithelial

scholarly journals Phospholipase D Activation by Sphingosine 1-Phosphate Regulates Interleukin-8 Secretion in Human Bronchial Epithelial Cells

2002 ◽  
Vol 277 (33) ◽  
pp. 30227-30235 ◽  
Author(s):  
Rhett J. Cummings ◽  
Narasimham L. Parinandi ◽  
Ari Zaiman ◽  
Lixin Wang ◽  
Peter V. Usatyuk ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Phospholipase D ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Sphingosine 1 Phosphate

scholarly journals Src-Mediated EGF Receptor Activation Regulates Ozone-Induced Interleukin 8 Expression in Human Bronchial Epithelial Cells

2015 ◽  
Vol 123 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Weidong Wu ◽  
Phillip A. Wages ◽  
Robert B. Devlin ◽  
David Diaz-Sanchez ◽  
David B. Peden ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Egf Receptor ◽  
Receptor Activation ◽  
Bronchial Epithelial Cells ◽  
Interleukin 8 ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial
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