The cloning and characterization of genes involved in amino acid metabolism in Neurospora crassa.

1993 ◽  
Author(s):  
Cynthia R. Davis
1988 ◽  
Vol 254 (3) ◽  
pp. F449-F449
Author(s):  
Itzhak Nissim ◽  
Beatrice States ◽  
Marc Yudkoff ◽  
Stanton Segal

Page F1243: Itzhak Nissim, Beatrice States, Marc Yudkoff, and Stanton Segal. “; Characterization of amino acid metabolism by cultured rat kidney cells: study with 15N.”Page F1243: the sentence beginning on the 14th line of the abstract should read: The disappearance of glutamine and glutamate was linear during a 60-min incubation at either pH. Page F1245: the last sentence in the legend of Figure 1 should read: Each data point is the mean ± SE of 4–6 experiments. Page F1246: Table 2, unit of measure was omitted, should be nmol·min-1·mg protein-1 Page F1249: the first full sentence in the left column should read: The data of Fig. 8 indicate that the formation of [6-amino]-15N-labeled adenine nucleotide is dependent on the H+ concentration of the incubation medium.


2000 ◽  
Vol 27 (5) ◽  
pp. 389 ◽  
Author(s):  
Jocelyn R. Eason ◽  
Jason W. Johnston ◽  
Leigh de Vré ◽  
Ben K. Sinclair ◽  
Graeme A. King

We have studied nitrogen metabolism during senescence of the ethylene-insensitive flower Sandersonia aurantiaca Hook. We report here on the isolation and characterization of two cDNAs from senescing sandersonia tepals. SAND1 encodes asparagine synthetase (AS; EC 6.3.5.4) and SAND3 encodes glutamine synthetase (GS; EC 6.3.1.2). The accumulation of SAND3 mRNAs was consistently high throughout flower development and senescence and was not associated with senescence per se. SAND1 mRNAs started to accumulate in mature flowers just prior to visual signs of senescence. Sucrose feeding of individual flowers delayed tepal senescence and altered amino acid metabolism. The levels of Asn, Gln and Glu were higher and Asp levels were lower in the sucrose-fed flowers. The senescence-associated peak in Gln was delayed in sucrose-fed flowers compared to controls that were held in water. Sucrose feeding had no effect on the initiation of SAND1 transcription at the onset of flower senescence; however, the later decline in SAND1 mRNA abundance was delayed in sucrose-fed flowers. Elimination of GS activity and the subsequent reduction in Gln levels caused by phosphinothricin treat-ment was associated with a delay of SAND1 mRNA accumulation in senescing flowers.


1979 ◽  
Vol 7 (1) ◽  
pp. 261-262
Author(s):  
E. V. ROWSELL

1985 ◽  
Vol 4 ◽  
pp. 141-146 ◽  
Author(s):  
K VESTERBERG ◽  
J BERGSTROM ◽  
P FURST ◽  
U LEANDER ◽  
E VINNARS

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