Integrating Backscatter X-Ray Imaging with Super-Resolution Imaging Algorithms for Plant Phenotyping

2019 ◽  
Author(s):  
J. Baciak ◽  
S. Cui ◽  
J. Nimmagadda ◽  
J. Ruiz-Munoz ◽  
A. Zare
Keyword(s):  
Super Resolution ◽  
Plant Phenotyping ◽  
X Ray ◽  
X Ray Imaging ◽  
Resolution Imaging ◽  
Imaging Algorithms

scholarly journals Multiscale X-ray imaging using ptychography

2018 ◽  
Vol 25 (4) ◽  
pp. 1214-1221 ◽  
Author(s):  
Simone Sala ◽  
Venkata S. C. Kuppili ◽  
Stefanos Chalkidis ◽  
Darren J. Batey ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Generation X ◽  
Imaging Techniques ◽  
X Rays ◽  
Widespread Application ◽  
Full Field ◽  
X Ray ◽  
X Ray Imaging ◽  
Imaging Mode ◽  
Resolution Imaging ◽  
Complementary Techniques

The success of ptychography and other imaging experiments at third-generation X-ray sources is apparent from their increasingly widespread application and the improving quality of the images they produce both for resolution and contrast and in terms of relaxation of experimental constraints. The wider availability of highly coherent X-rays stimulates the development of several complementary techniques which have seen limited mutual integration in recent years. This paper presents a framework in which some of the established imaging techniques – with particular regard for ptychography – are flexibly applied to tackle the variable requirements occurring at typical synchrotron experiments. In such a framework one can obtain low-resolution images of whole samples and smoothly zoom in on specific regions of interest as they are revealed by switching to a higher-resolution imaging mode. The techniques involved range from full-field microscopy, to reach the widest fields of view (>mm), to ptychography, to achieve the highest resolution (<100 nm), and have been implemented at the I13 Coherence Branchline at Diamond Light Source.

scholarly journals X-Ray Imaging: Status and Trends

1987 ◽  
Vol 31 ◽  
pp. 35-52 ◽  
Author(s):  
R.W. Ryon ◽  
H.E. Martz ◽  
J.M. Hernandez ◽  
J.J. Haskins ◽  
R.A. Day ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Rapid Progress ◽  
Optical Elements ◽  
Materials Analysis ◽  
X Ray ◽  
X Ray Imaging ◽  
Status And Trends ◽  
Resolution Imaging ◽  
Material Fabrication

There is a veritable renaissance occurring in x-ray imaging. X-ray imaging by radiography has been a highly developed technology in medicine and industry for many years. However, high resolution imaging has not generally been practical because sources have been relatively dim and diffuse, optical elements have been nonexistant for most applications, and detectors have been slow and of low resolution. Materials analysis needs have therefore gone unmet. Rapid progress is now taking place because we are able to exploit developments in microelectronics and related material fabrication techniques, and because of the availability of intense x-ray sources.

scholarly journals Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG

2016 ◽  
Author(s):  
Julian Ng ◽  
Alyssa Browning ◽  
Lorenz Lechner ◽  
Masako Terada ◽  
Gillian Howard ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Neural Circuit ◽  
Cell Types ◽  
Cellular Tissue ◽  
Computer Assisted ◽  
X Ray ◽  
X Ray Imaging ◽  
Resolution Imaging ◽  
Volume Acquisition ◽  
Non Destructive

AbstractLarge dimension, high-resolution imaging is important for neural circuit visualisation as neurons have both long- and short-range patterns: from axons and dendrites to the numerous synapses at their endings. Electron Microscopy (EM) is the favoured approach for synaptic resolution imaging but how such structures can be segmented from high-density images within large volume datasets remains challenging.Fluorescent probes are widely used to localise synapses, identify cell-types and in tracing studies. The equivalent EM approach would benefit visualising such labelled structures from within sub-cellular, cellular, tissue and neuroanatomical contexts.Here we developed genetically-encoded, electron-dense markers using miniSOG. We demonstrate their ability in 1) labelling cellular sub-compartments of genetically-targeted neurons, 2) generating contrast under different EM modalities, and 3) segmenting labelled structures from EM volumes using computer-assisted strategies. We also tested non-destructive X-ray imaging on whole Drosophila brains to evaluate contrast staining. This enables us to target specific regions for EM volume acquisition.

scholarly journals Shack–Hartmann wavefront sensors based on 2D refractive lens arrays and super-resolution multi-contrast X-ray imaging

2020 ◽  
Vol 27 (3) ◽  
pp. 788-795 ◽  
Author(s):  
Andrey Mikhaylov ◽  
Stefan Reich ◽  
Margarita Zakharova ◽  
Vitor Vlnieska ◽  
Roman Laptev ◽  
...  
Keyword(s):  
Super Resolution ◽  
Continuous Exposure ◽  
X Rays ◽  
Contrast Imaging ◽  
Wavefront Sensors ◽  
Shape Modification ◽  
Two Photon ◽  
X Ray Imaging ◽  
Resolution Imaging ◽  
First Time

Different approaches of 2D lens arrays as Shack–Hartmann sensors for hard X-rays are compared. For the first time, a combination of Shack–Hartmann sensors for hard X-rays (SHSX) with a super-resolution imaging approach to perform multi-contrast imaging is demonstrated. A diamond lens is employed as a well known test object. The interleaving approach has great potential to overcome the 2D lens array limitation given by the two-photon polymerization lithography. Finally, the radiation damage induced by continuous exposure of an SHSX prototype with a white beam was studied showing a good performance of several hours. The shape modification and influence in the final image quality are presented.

Achieving super-resolution X-ray imaging with mobile C-arm devices

2009 ◽  
Vol 5 (3) ◽  
pp. 243-256 ◽  
Author(s):  
C. Bodensteiner ◽  
C. Darolti ◽  
A. Schweikard
Keyword(s):  
Super Resolution ◽  
X Ray ◽  
X Ray Imaging
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