scholarly journals Anthelmintic effects of pumpkin (Cucurbita moschata) seed extract on Ascaris suum in vitro

2017 ◽  
Vol 10 (1) ◽  
pp. 1-6
Author(s):  
SHITA GANESTYA ◽  
SUTARMIADJI DJUMARGA ◽  
CR. SITI UTARI
Keyword(s):  
Ascaris Suum ◽  
Sampling Technique ◽  
Seed Extract ◽  
Cucurbita Moschata ◽  
Spearman Correlation ◽  
Pumpkin Seed ◽  
Death Time ◽  
Significant Difference ◽  
Seed Extracts

Ganestya S, Djumarga S, Utari CRS. 2012. Anthelmintic effects of pumpkin (Cucurbita moschata) seed extract on Ascaris suum in vitro. Biofarmasi 10: 1-6. The aim of this research was to determine the effect of pumpkin seed extract towards the Lethal Death Time of Ascaris suum Goeze in vitro. The type of this research was an quasi-experiment using a post-test only controlled group design. The subject of this research was the actively motile adult Ascaris suum Goeze. A purposive sampling technique was used to collect the samples by considering the length of each worm without noticing the sex of worm. The subjects were divided into four groups, each group consisted of 10 worms, with 5 times replication. Physiological saline solution and pyrantel pamoate 0.236% were used as the negative and positive control groups, respectively, while the pumpkin seed extracts used for the treatment groups were at the concentrations of 54.5% and 70.5%. The observation and checking towards the dead worm were performed once an hour. The data obtained were analyzed by using a Kruskal-Wallis test, continued with Mann-Whitney and Spearman Correlation tests. The Lethal Death Time of A. suum by pumpkin seed extracts at a concentration of 54.5% was 11 hour 48 minutes, while at a concentration of 70.5%, the Lethal Death Time was 7 hours 48 minutes. The result of Kruskal-Wallis test showed a significant difference among all of the groups (p<0.05). A significant difference was also shown at every group by Mann-Whitney test (p<0.05). Meanwhile, the result of Spearman correlation test showed a significant negative correlation between the concentration of pumpkin seed extract and the Lethal Death Time as p<0.05 and coefficient correlation was -0.950. Pumpkin seed extract showed an anthelmintic effect against A. suum in vitro.

scholarly journals The Anthelmintic Effect of Pumpkin Seed (Cucurbita moschata Durch) Infusion on Lethal Death Time of Fasciola giganticaIn Vitro

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Andriani Dwi Siswarini ◽  
Kusnoto Kusnoto ◽  
Retno Bijanti
Keyword(s):  
Negative Control ◽  
Fasciola Gigantica ◽  
Cucurbita Moschata ◽  
Pumpkin Seed ◽  
Death Time ◽  
Significant Difference ◽  
Breeding Animal ◽  
Range Test ◽  
Anthelmintic Effect

Fasciola gigantica is a worm which could infect breeding animal and human. One of the plants that can be used as worm drug is Pumpkin seed (Cucurbita moschata Durch).Pumpkin seed (Cucurbita moschata Durch) has been used in traditional anthelmintic medicine. This research was conducted to determine the anthelmintic effect of Pumpkin seed (Cucurbita moschata Durch) infusion on lethal death time of Fasciola gigantica in vitro. This research used Fasciola gigantica 25-75 mm in length without differentiating their sex. The concentrations of Pumpkin seed (Cucurbita moschata Durch) for treatment of Fasciola gigantica were 25%, 50% and 75%. The negative control used NaCl physiology (0.9 %). ANOVA factorial test showed significant difference among all of the experiment groups (p <0.05). Duncan multiple range test was seen the best treatment was the shortest lethal death time in concentration of 75%.Six hours of the treatment was the best time for making death of the worm. The Pumpkin seed (Cucurbita moschata Durch) in 75% dose was the best dose for making death of the worm. In 6th hours of the treatment in 75% dose administering had the best for making death of Fasciola gigantica. As for the reason, pumpkin seed (Cucurbita moschata Durch) can be use as anthelmintic. 

scholarly journals In vitro health beneficial activities of Pumpkin seeds from Cucurbita moschata cultivated in Lemnos

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Danai Sakka ◽  
Haralabos C. Karantonis
Keyword(s):  
Platelet Activating Factor ◽  
Health Improvement ◽  
Cucurbita Moschata ◽  
Pumpkin Seed ◽  
Anti Oxidant ◽  
Pumpkin Seeds ◽  
Anti Inflammatory ◽  
Locally Grown ◽  
Seed Extracts

Pumpkin seeds are commonly consumed in Greece. Although Cucurbita moschata is locally grown in Lemnos and is traditionally used in pumpkin pies, the seeds are currently discarded after consumption of the fruit flesh. The aim of the present study was to investigate the nutritional functionality of pumpkin seeds from Cucurbita moschata grown in Lemnos.Cucurbita moschatas’ seeds, raw or roasted, were appropriately extracted and the results are presented for raw versus (vs) roasted seed extracts. The phenolic content was expressed as μg gallic acid/g of seeds according to Folin-Ciocalteau assay (370.3 ± 19.1 vs 551.0 ± 22.0). Antioxidant capacity was expressed as equivalent amount for 50% scavenging in mg of seeds for DPPH (50.03 ± 5.91 vs 25.82 ± 6.77) and ABTS (17.85 ± 0.77 vs 12.77 ± 0.76) assays, and as μmol of trolox/g of seeds for FRAP (1.19 ± 0.05 vs 2.50 ± 0.23) and CUPRAC (2.13 ±0.11 vs 3.25 ± 0.06) assays. Anti-inflammatory/anti-thrombotic and anti-diabetic activities were expressed as mg of seeds for 50% inhibition of platelet activating factor (0.62 vs 0.15) and as μg of seeds for 25% inhibition of alpha-glycosidase (40.0 vs 61.0) activities respectively. Moreover, anti-atherogenic activity was expressed as the % increase in lag time of human plasma oxidation (62.7 versus 163.2)Raw and roasted pumpkin seed extracts exert anti-oxidant, anti-thrombotic/anti-inflammatory, anti-atherogenic and antidiabetic activities. Cucurbita moschata seeds may represent a novel opportunity for development of functional foods, with a local interest in Lemnos that would contribute also to the regional public health improvement.

scholarly journals Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

2011 ◽  
Vol 9 (2) ◽  
pp. 33-37
Author(s):  
MUHAMMAD ARIF NUR SYAHID ◽  
CR. SITI UTARI ◽  
SUTARMIADJI DJUMARGA
Keyword(s):  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mimosa Pudica ◽  
Control Group Design ◽  
Significance Level ◽  
Death Time ◽  
Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

scholarly journals Screening of some Leguminosae seeds for nematicidal activity

2004 ◽  
Vol 22 (1) ◽  
pp. 51 ◽  
Author(s):  
Uma R Khurma ◽  
Anupama Mangotra
Keyword(s):  
Seed Extract ◽  
Sesbania Sesban ◽  
Nematode Control ◽  
Recent Past ◽  
Cassia Fistula ◽  
Pongamia Glabra ◽  
Meloidogyne Spp ◽  
Alternative Sources ◽  
Seed Extracts

Root-knot nematodes, Meloidogyne spp., are the major nematode pests of economic crops worldwide. Use of conventional nematicides has been one of the most effective methods to reduce their populations. However, the ban on many effective nematicides in recent past has necessitated the search for alternative sources of effective and ecofriendly chemicals for nematode control. The nematicidal potential of fifteen Leguminosae seeds (wild and cultivated) was assessed, in vitro, against juveniles of Meloidogyne incognita (Kofoid & White) Chitwood. Each standard aqueous seed extract, 25g/100ml (w/v), was evaluated at dilutions, 1:10, 1:20, and 1:40 at 27+1�C for 24 hours. The majority of seed extracts caused high mortality rates, especially in 1:10 concentration. Trigonella foenum graceum, Sesbania sesban, Albizzia lebbak, Cassia fistula and Pongamia glabra were the most efficacious (above 90% mortality). S. sesban and P. glabra were significantly effective even in the lowest concentration (1:40).

scholarly journals EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

2017 ◽  
Vol 9 (5) ◽  
pp. 29
Author(s):  
Iswariya S. ◽  
Uma T. S.
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Seed Extract ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Anti Inflammatory ◽  
Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

scholarly journals In vitro antioxidant and antidiarrheal activities of aqueous and n-hexane extracts of Cucurbita maxima seed in castor oil-induced diarrheal rats

2022 ◽  
Author(s):  
Habibu Tijjani ◽  
Adamu Matinja ◽  
Marwanatu Yahya ◽  
Emmanuel Aondofa ◽  
Akibu Sani
Keyword(s):  
Vitamin C ◽  
Aqueous Extract ◽  
Castor Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Seed Extract ◽  
Hexane Extract ◽  
Cucurbita Maxima ◽  
Seed Extracts

Diarrhea is a common health complaint occurring with mild, temporary conditions to a potentially life-threatening condition. <i>Cucurbita maxima</i> (Cucurbitaceae) seed is reportedly used traditionally for the treatment of diarrheal and thus this study aimed to evaluate the in vitro antioxidants, total tannins, phenolics contents, and antidiarrheal potentials of <i>n</i>-hexane and aqueous <i>C. maxima</i> seed extracts in castor oil induced diarrheal rats. The <i>n</i>-hexane extract mainly contains oils while the aqueous extract was thick, brown solid. The aqueous and <i>n</i>-hexane seed extract of <i>C. maxima</i> expressed significant 2,2, diphenyl-1-picrythdrazyl (DPPH) scavenging activities at 6.25 – 1000 mg/mL with IC<sub>50</sub> values of 104.01 mg/mL, 29.27 mg/mL and 26.78 mg/mL for <i>n</i>-hexane, aqueous and vitamin C respectively. The hydroxyl radical scavenging activities of the <i>n</i>-hexane and aqueous seed extract of <i>C. maxima</i> were significantly lower at higher concentrations compared with that of vitamin C. Furthermore, the total antioxidant capacities of <i>n</i>-hexane (22.08mg/mL) and aqueous seed extract (11.03 mg/mL) of <i>C. maxima</i> were found to be higher than that of vitamin C (134.46 mg/mL). The aqueous extracts (658.33±380.08 mg QE/g) total tannins were not significantly different from the <i>n</i>-hexane extract (468.33±102.55 mg QE/g) while the <i>n</i>-hexane extract contains significantly higher total phenolics (2.93±1.25 mg GAE/g) compared with the aqueous extract (0.19±0.04 mg GAE/g). Percentage inhibition of stooling was found to be 57±22%, 41±16%, and 46±11% for loperamide, <i>n</i>-hexane, and aqueous respectively. The study concludes that aqueous and n-hexane seed extracts of <i>C. maxima</i> (pumpkin) possess in vitro antioxidant activities and antidiarrheal properties.

scholarly journals Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro

2011 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
OKKIE MHARGA SENTANA ◽  
SRI HARYATI ◽  
YUL MARIYAH
Keyword(s):  
Linear Regression ◽  
Ascaris Suum ◽  
Linear Regression Analysis ◽  
Negative Relationship ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Control Group ◽  
Ocimum Americanum

Sentana OM, Haryati S, Mariyah Y. 2011. Effects of anthelminthic of ethanol extract of basil (Ocimum americanum) leaf against Ascaris suum death in vitro. Biofarmasi 9: 1-6. This research aimed to examine the relationship between the ethanol extract of basil leaf against Ascaris suum mortality rate in vitro. This study was a laboratory experiment with a research design of the post-test with controlled group design. The samples were Ascaris suum Goeze that still actively moved and obtained from pig intestines. The sampling technique used was a purposive sampling by equating the type and the length of worms, and it did not distinguished for the sex of worms. The subjects were divided into five groups, each group consisted of 5 worms and repeated in 6 times. The control group contained 25 mL of physiological saline solution and 25 mL pyrantel pamoate 5 mg/mL. Three other groups contained basil leaves extracts consisted of various concentrations, i.e. 30%, 40% and 50%. The number of dead worms was counted every 2 hours until all the worms in the group died. The data were analyzed by using a linear regression analysis and a probit analysis. Simple linear regression statistical test showed a significance correlation at 0.000 with 0.837 correlation coefficient was negative, R square 0.701, in which the significant correlation <0.05 or the value of 37.565 with a significance of means, there was a negative relationship between the length of time of death of worms with large concentrations of basil extract. Analysis using a probit analysis obtained LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours. The ethanol extract of basil leaf could affect the death of A. suum in vitro with LC50 at a concentration of 40% and LT50 at a concentration of 40% was 2 hours.

scholarly journals Parental Involvement and Children’s Academic Achievement: In the Case of Some Selected Government Primary Schools Bahirdar City, Ethiopia

2019 ◽  
Vol 2 (1) ◽  
pp. p43
Author(s):  
Bezabih, Mezgebu Bayu
Keyword(s):  
Academic Achievement ◽  
Parental Involvement ◽  
Primary Schools ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Spearman Correlation ◽  
Significant Difference ◽  
Parental Level ◽  
The Relationship ◽  
One Way Anova

The purpose of this study was to examine the relationship between parental involvement and academic achievement of children in some selected full cycle government primary schools of Bahirdar city. To achieve this objective, 180 sample students were selected by using simple random sampling technique. The collected data was analyzed through, spearman correlation, analysis of variance and independent-test. The finding of the study revealed that the statistical analysis on independent t-test showed that there was a statistically significant difference between male and female students with regard to their academic achievement (t=6.25, df = 178, p<0.05). The finding of spearman correlation indicates that there was significant relationship between parental involvement and academic achievement of students (r=.56, p <.05). The result on one way ANOVA displayed that there was statistical significant difference among respondents academic achievement with regard to their parental level of education (F 2, 176) = 17.084, P<0.05. In conclusion, parental involvement is positively correlated with academic achievement of students and males have better academic performance than their female counter parts.

scholarly journals Phytochemical Screening, Mathematical Analysis and Antimicrobial Activity of Methanolic Seed Extract of Hunteria Umbellata

2020 ◽  
pp. 1-17
Author(s):  
Oluwaseun Raphael Aderele ◽  
Adekunle Kareem Rasaq ◽  
Johnson Oshiobugie Momoh
Keyword(s):  
Antimicrobial Activity ◽  
Seed Extract ◽  
T Test ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
In Vitro Antimicrobial Activity ◽  
E Coli ◽  
Significant Difference ◽  
Agar Well Diffusion Method

Aim: The study evaluates the in-vitro antimicrobial activity of Hunteria umbellata against Escherichia coli, Staphylococcus aureus and Streptococcus sp. Place and Duration of Study: The study was carried out for three months in 2019 in Biochemistry Laboratory, Department of Chemical Sciences (Biochemistry unit), School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos- Nigeria. Methodology: The qualitative and GC-MS analysis of Hunteria umbellata methanolic seed extract were determined using standard procedure. The antimicrobial activity was evaluated by the disc diffusion method and agar well diffusion method. The experimental data was resampled 1000 times to allow for higher degrees of freedom in carrying out t-test to test for the difference of the effect of in-vitro antimicrobial activity of H. umbellata against E. coli, S. aureus and Streptococcus sp using mathematical software R language (3.6.1 version). Line plots, histogram and t-test are used to explain the effect of antimicrobial activity of H. umbellate on the selected bacteria. MIC and MBC were determined using standard methods. Results: The Phytochemical analysis of methanolic seed extract of Hunteria umbellata showed the presence of secondary metabolites like saponins, tannins, flavonoids, steroids, phenol among others. GC-MS assay of the H. umbellata seed extract revealed the presence of eight different compounds. Agar well diffusion method was characterized by inhibition zones of 18.36±0.87, 19.13±1.03 and 21.62±2.53 mm for E.coli, S. aureus and Streptococcus sp respectively at 300 mg/ml-1 and 21.70± 1.60, 23.83± 2.64 and 28.57± 1.52 for E.coli, S. aureus and Streptococcus sp respectively at 500 mg/ml. The results of the analysis show that there is a significant difference between the effects of in-vitro antimicrobial activity of H. umbellate on 3001 and 500 mg/ml on each bacteria tested at 5% level of significance. E.coli, S. aureus and Streptococcus sp were tested against 12 standard antimicrobial agents, of which six was sensitive and another six was resistance to E .coli, seven was sensitive, and five was resistance to S. aureus while four was resistance and eight sensitive to Streptococcus sp. The minimum inhibitory concentration (MIC) for E.coli, S. aureus, and  Streptococcus sp were 250, 125 and 31.25 mgml-1 while their minimum bactericidal concentration (MBC) were 500, 250 and 125 respectively. MIC and MBC tests showed that H. umbellata methanolic seed extract had noticeable bactericidal effects with MBC/MIC values ranging between 2 to 4. The extract has strong potency against these microorganisms with Streptococcus sp being the most susceptible. Conclusions: Hunteria umbellata has potential as natural therapeutic agents against E. coli, S. aureus and Streptococcus sp and they may prevent pathogenic diseases.

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