The effect of meniran (Phyllanthus niruri) extract protection on mice gastric histology damage induced by aspirin

2010 ◽  
Vol 8 (2) ◽  
pp. 41-46
Author(s):  
PEDIANA RACHMAWATI ◽  
ENDANG LISTYANINGSIH SUPARYANTI ◽  
ISDARYANTO ISDARYANTO
Keyword(s):  
Treatment Group ◽  
Mus Musculus ◽  
Statistical Tests ◽  
Statistical Test ◽  
Laboratory Research ◽  
Control Group ◽  
Phyllanthus Niruri ◽  
Group I ◽  
Significant Difference ◽  
Per Oral

Rachmawati P, Suparyanti EL, Isdariyanto. 2010. The effect of meniran (Phyllanthus niruri) extract protection on mice gastric histology damage induced by aspirin. Biofarmasi 8: 41-46. This experiment aimed to determine the effect of meniran (Phyllanthus niruri Linn.) extract protection on mice (Mus musculus) gastric histology damage induced by aspirin. This study belongs to an experimental laboratory research with post-test only controlled group design. The tested animals used were 25 Swiss Webster male mice in 6-8 weeks age and ±20 g body weight, divided into 5 groups: (i) control group (K) that given with 0.2 mL CMC, Na 0.5% and 0.1 mL aquadest, (ii) treatment group I (P1) that given with 0.2 mL aquadest and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, (iii) treatment group II (PII) that given with 0.2 mL meniran extract with a dosage of 1.3 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, (iv) treatment group III (PIII) that given with 0.2 mL meniran extract with a dosage of 2.6 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral, and (v) a positive control group (PIV) that given with 0.2 Cimetidine with a dosage of 2.6 mg/20 g BW per oral and 0.1 mL aspirin with a dosage of 1.7 mg/20 g BW mice per oral. The gastric histological description could be seen with a bright light microscope with 100x magnification followed by 400x magnification. Then, the gastric histological description was divided into normal, light damage, and heavy damage. The data obtained were analyzed by using Kruskal Wallis and Mann Whitney statistical tests at α = 0.05. The results of Kruskal Wallis statistical test showed that there was at least one population with a value that higher than other population. The result of Mann Whitney statistical test showed that there was a significant difference between groups K-PI, PI-PII, PI-PIII, and PI-PIV, and no significant difference between K-PII, K-PIII, K-PIV, PII-PIII, PII-PIV, and PIII-PIV. The conclusion of the research was the meniran (Phyllanthus niruri Linn.) extract can give a protection on mice (Mus musculus) gastric histological damage induced by aspirin.

scholarly journals Changes in the number of macrophage and lymphocyte cells in chronic periodontitis due to dental X-ray exposure

2018 ◽  
Vol 51 (2) ◽  
pp. 99 ◽  
Author(s):  
Alhidayati Asymal ◽  
Eha Renwi Astuti ◽  
Rini Devijanti
Keyword(s):  
Treatment Group ◽  
Chronic Periodontitis ◽  
Radiographic Examination ◽  
Control Group ◽  
X Ray ◽  
The Third ◽  
Macrophage Cells ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

Background: Periodontitis is an inflammatory disease caused by specific microorganisms that attacks tooth-supporting tissues, P. gingivalis bacteria are mostly found in patients suffering from chronic periodontitis which is usually diagnosed by means of clinical and radiographic examination. The latter play important roles in the management of periodontitis, including: establishing diagnosis, determining treatment plans and evaluating the results of treatment. Unfortunately, the use of X-rays to perform such radiographic examination has negative effects since the body’s various parts, especially the head, are not well protected from the effects of X-ray radiation. Purpose: This research aimed to analyze the effects of dental X-ray exposure on the number of macrophages and lymphocytes in experimental subjects suffering from periodontitis. Methods: 36 rats that had been diagnosed with chronic periodontitis were divided into three groups, namely: a control group, treatment group I (exposed to a 0.16 mSv dose of radiation) and treatment group II (exposed to a 0.32 mSv dose of radiation). These subjects were subsequently sacrificed on the third and fifth days after treatment. Thereafter, histopathological examination was performed to identify any changes in the number of macrophages and lymphocytes. Results: The results of an HSD test confirmed that, on the third day, there were significant differences in the number of lymphocytes between the control group and treatment group I, as well as between the control group and treatment group II. On the fifth day, there were also significant differences in the number of lymphocytes between the control group and treatment group I, as well as between treatment group I and treatment group II. Similarly, there was a significant difference in the number of macrophage cells on the third day between the control group and treatment group I. On the fifth day, there were also significant differences in the number of macrophage cells between the control group and treatment group I, as well as between treatment group I and treatment group II. Conclusion: Dental x-ray exposure at a dose of 0.16 mSv can elevate the number of macrophages and lymphocytes on the third and fifth days. On the other hand, dental x-ray radiation at a dose of 0.32 mSv can reduce the number of macrophages on day 3 as well as the number of lymphocytes on the third and fifth days.

scholarly journals PENGARUH PEMBERIAN GEL AIR KELAPA GADING (COCOS CAPITATA) TERHADAP TINGKAT KECEMASAN PADA HIPERSENSITIVITAS DENTIN Studi in vivo terhadap tikus wistar jantan

2016 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
Chariza Hanum Mayvita Iskandar ◽  
Yayun Siti Rochmah ◽  
Arlina Nurhapsari
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Positive Control ◽  
Anxiety Level ◽  
Positive Control Group ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Group I ◽  
Significant Difference

Background: Ivory palm water (Cocos capitata) has a high potassium content. Potassium ion can depolarized excited dental nerve on dentin hypersensitivity. This study was to determine the effect of water gel ivory palm against dentine hypersensitivity by the anxiety level of mice using RGS (Rat Grimace Scale).Method: This research has been through ethical clearance which researchmethod was quasy experimental in vivo. The sample was 16 Wistar rats (Rattus novergicus) male 4-5 months with 4 mice in each group. Group I as a positive control. Group II as the ivory palm water gel 10% treatment group. Group III as the ivory palm water gel 70% treatment group. Group IV as a negative control. Research data analysis used Kruskal Wallis test with p <0.05 then continued by Mann Whitney test.Result: Kruskal Wallis test obtained significant difference between the four study groups (p <0.05). Mann Whitney test results in comparison either between positive control group and ivory palm water gel 10% and 70%, and comparison group of ivory palm water gel 10% and 70% weren’t significantly different.Conclusion: This study conclusion there was no significant difference between ivory palm water gel 10 % and 70 % in reducing anxiety level in male Wistar rats with dentinal hypersensitivity

scholarly journals PENGARUH PEMBERIAN SEDUHAN DAUN ASAM JAWA ( Tamarindus indica L.) TERHADAP PENURUNAN KADAR GULA DARAH PADA MASYARAKAT DESA TANJUNGBUMI KABUPATEN BANGKALAN MADURA

2021 ◽  
Vol 3 (2) ◽  
pp. 71-85
Author(s):  
arista wahyu ningsih ◽  
◽  
Nur Arzy Samsiyeh ◽  
Martina Kurnia Rohmah ◽  
◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Treatment Group ◽  
Blood Sugar ◽  
Statistical Tests ◽  
T Test ◽  
The Body ◽  
Control Group ◽  
Tamarindus Indica ◽  
Significant Difference ◽  
Sugar Levels

Diabetes mellitus is a disease or metabolic disorder that is most often found in society, including one of the diseases or metabolic disorders. Tamarind leaves (Tamarindus indica L) is a plant that can be used as an alternative treatment for Diabetes Mellitus which contains flavonoids, sapoin and tannins, which can lower glucose levels and inhibit glucose absorption in the blood. This study aims to determine the differences in blood sugar levels before and after giving tamarind leaves infusion in the village community of Tanjung Bumi. This type of research is Quasy Experiment Design and purposive sampling technique with a sample of 29 respondents. Based on the results of the paired t-test statistical analysis showed a significant difference (p = 0.000) between the blood sugar levels of the group and placebo. Meanwhile, in the posttest the treatment group after giving tamarind leaves showed the same as the control group, namely p = 0.000, which means that there is also a difference in the decrease in blood sugar levels between the sebelum pemberian perlakuan and posttest. The results of statistical tests with the Independent T-Test showed the results in both groups with a value of p = 0.000, meaning that there was a difference in the decrease in blood sugar levels between the control group and the treatment group. Steeping tamarind leaves has an effect in reducing blood sugar levels in the body

scholarly journals Determination of Highest Dose of Ammonia without Effect at Work Environment through the Expression of Interleukin-2 Cell in Rattus Novergicus

2019 ◽  
Vol 7 (6) ◽  
pp. 897-902
Author(s):  
Abdul Rohim Tualeka ◽  
Dwi Ananto Wibrata ◽  
Ahsan Ahsan ◽  
Pudji Rahmawati ◽  
Syamsiar S Russeng ◽  
...  
Keyword(s):  
Body Weight ◽  
Statistical Tests ◽  
Interleukin 2 ◽  
Threshold Limit Value ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Limit Value ◽  
Significant Difference

BACKGROUND: For determining the threshold limit value firstly determined the highest dose of ammonia without effect (NOAEL). However, research on the determination of NOAEL ammonia didn't conduct in Indonesia. AIM: The aim of this study to determine the value of the highest dose of ammonia without effect (No Observed Adverse Effect Level/NOAEL) through interleukin-2 (IL-2) expression on white mice. METHODS: This study used experimental laboratory research with post-test only control group design using white mice as experimental subjects. The treatment group divided into 6 groups (a group of controls and five groups with different ammonia exposure through inhalation). The trend curve of Remmele Scale Index (IRS) and histopathologic analysis could be used for determining NOAEL. RESULT: The location of the highest dose of ammonia without any effect (NOAEL) of white mice was in the second group, with 0.0103 mg/kg body weight dose. Analysis of statistical tests Kruskal Wallis stated there was no significant difference in interleukin-2 expression between the control with ammonia exposed group with a significance of p (0.747) > α (0.05). CONCLUSION: There is no difference between some interleukin-2 expression in the lymphocyte cell lung white mice group exposed to ammonia and control group. The highest dose of ammonia without any effect (NOAEL) on white mice was 0.0103 mg/kg body weight.

scholarly journals PENGARUH PERASAN UMBI AKAR LOBAK (Rhapanus sativus L) TERHADAP PENINGKATAN KADAR PROLAKTIN DALAM DARAH MENCIT (Mus musculus) BETINA

2006 ◽  
Vol 11 (2) ◽  
pp. 135-137
Author(s):  
Mas Loegito ◽  
Paulus Liben ◽  
Thin Soedarti
Keyword(s):  
Mus Musculus ◽  
Prolactin Level ◽  
Day Treatment ◽  
Control Group ◽  
Treatment Groups ◽  
Female Mice ◽  
Randomized Design ◽  
Significant Difference ◽  
Per Oral ◽  
Tuber Extract

The objective of this research was to prove that the administration of radish tuber extract (Rhapanus sativus L) to female mice (Mus musculus) might increase blood prolactin level. This was an experimental research using complete randomized design by involving three treatment groups and three control group, each with five replications. Materials consisted of radish tuber extract and 30 female mice of 8 weeks old divided into 6 groups, i.e., control groups of C1, C2, and C3, and treatment groups of T1, T2, and T3. C1, C2, and C3 groups were given water of 1x 0.5 cc/day per oral, 2x0.5 cc/day, and 3x0.5 cc/day respectively. Treatment groups of T1, T2, and T3 were given radish tuber extract of respectively 1x0.5 cc/day per oral, 2x0.5 cc/day, and 1x0.5 cc/day. Treatment was carried out for 21 days. Blood was subsequently taken from those rats for blood prolactin level. Data were analyzed using Anove test. If the results indicated significant difference of 95 percent (p = 0.05), the analysis was continued with LSD. The conclusions of this experiment is the administration of radish tuber extract per oral may result in the increase of blood prolactin level.

scholarly journals Gambaran Histologi Ginjal Tikus Wistar Yang Terpapar MSG Setelah Perlakuan Diberikan Jus Tomat Dan Diberhentikan Perlakuan Saja

2018 ◽  
Vol 1 (2) ◽  
pp. 62
Author(s):  
Yeti Eka Sispitasari
Keyword(s):  
Treatment Group ◽  
The Body ◽  
Proximal Tubules ◽  
Tomato Juice ◽  
Control Group ◽  
Renal Tubules ◽  
Apparent Difference ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

MSG causes many side effects on the body, but MSG has long been used as a food flavor that can bring tastes (umami) and play a role in strengthening the taste. The chemical structure of MSG is no different from Glutamic Acid (glutamate), it is one of the 20 amino acids that make up proteins in the body. So the use of MSG needs to be discontinued to prevent kidney damage due to continued consumption of MSG, two research were conducted to determine the effect of discharging of MSG consumption by being given tomato juice (1) and discharged without treatment (2), the second study was seen from the histology picture of rats wistar mouse.Methods were experimental with post test only control group design. The research (1) used 15 wistar rats divided into three groups: group I without treatment (pellet AD II and drinking water), group II was given MSG for 14 days and group III was given tomato juice and MSG for 14 days. The study (2) used 27 rats divided by 9 groups. The sample is selected by simple random sampling method. The mice were then gradually turned off on the 29th, 43rd and 57th days. There was no significant difference in mean number of normal and damaged proximal tubules in all treatment groups. The renal histologic features in the treatment group I (MSG) and the treatment group II (MSG and tomato juice) showed normal glomeruli, tubular epithelial tubules, and lumen tubular narrowing whereas in mice discharged without treatment there was no apparent difference between the two MSG Giving groups damage to proximal tubules and renal corpusculum and regeneration after 14 days of discontinuation of MSG. Conclusions from these two experiments occurred damage to the renal tubules Keywords: MSG, Kidney, Wistar Rat

scholarly journals Acute Toxicity Test of Gedi (Abelmoschus manihot L.) Ethanol Leaves Extract to Histopatology of Liver Mice

MEDULA ◽  
2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Mutmainna Mutmainna ◽  
Yenti Purnamasari ◽  
Parawansah Parawansah
Keyword(s):  
Treatment Group ◽  
Acute Toxicity ◽  
Liver Tissue ◽  
Histopathological Examination ◽  
Liver Cells ◽  
Control Treatment ◽  
Control Group ◽  
Group I ◽  
Significant Difference ◽  
Mice Liver

ABSTRACTBackground: Abelmoschus manihot L.are widely used  as a daily dish  of dishes and traditional medicine to treat various diseases. It contains alkaloids, triterpenoids, saponins, and flavonoids wich can be toxic agent at variable dose. Purpose: This study aimed determine the effect of ethanol extract of abelmoschus manihot L. on histopatologic image of mice liver tissue.  Methods: This is quasi experimental study  with post test only control group design. Fourty five mice  (mus musculus)  as the subject of this research, they divided into 5 groups, group I threated by 50 mg /kgBB extract, group II threated by 200 mg / kgBB extract, group III threated by 800 mg / kgBB extract, group IV threated by 3,200 mg / kgBB extract, and group V as negative control. Treatment was done for 14 days and terminated at next day for histopatology examination use them liver tissue. Result: Histopathological examination of liver cells within normal limits. In the treatment group of 50 mg liver tissue of mice who died during treatment on day 7 and day 10 showed liver tissue necrosis. In the treatment group of 200 mg and 800 mg of liver tissue showed the presence of liver cell necrosis, and cell boundaries appeared unclear. In the treatment group of 3200 mg liver tissue showed necrotic cells, lysi s cells, unclear  cell  boundaries  and  necrotic  cells  more  widely.Conclusion:  As  a  conclusion  there  an histopatologic changes of  microscopic examination of mice liver cells in the form of necrosis and, there is not significant difference in ALT and AST  between the control group.Keywords : Abelmoschus manihot L. extract, acute toxicity, liver histopathology

scholarly journals Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis

2010 ◽  
Vol 8 (2) ◽  
pp. 47-51
Author(s):  
NUR AMALINA ◽  
SUYATMI SUYATMI ◽  
ENDANG LISTYANINGSIH SUPARYANTI
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Leaf Extract ◽  
Study Groups ◽  
Control Group ◽  
High Concentration ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
The Difference

Amalina N, Suyatmi, Suparyanti EL. 2010. Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis. Biofarmasi 8: 47-51. This research aimed to determine the effect of beluntas (Pluchea indica L.) leaf extract in habiting spermatogenesis. Flavonoids can inhibit aromatase enzyme that function to catalyze the conversion of androgens into estrogen so that the level of the testosterone hormone will increase. The high concentration of testosterone will affect on the feedback to the pituitary, that not releasing FSH and LH, so it will inhibit spermatogenesis. This research is an experimental research with post-test only controlled group design method, using male mice, 2-3 months age, weight 20-30 g, as many as 24 mice divided into 4 groups. The first group was a control group, the second group as treatment group I that given with 1.4 mg/20 g body weight beluntas extract, the third group as treatment group II that given with 2.8 mg/g body weight beluntas extract. The fourth group as treatment group III that given with 5.6 mg/g body weight beluntas extract. The treatment was given for 10 days after that the testes of mice were taken and from each testicle was made 3 slices and from each slice the most representative seminiferous tubule was taken for spermatid cells. So, each mice had 18 units of data would be analyzed. The data obtained were analyzed with Anova to determine the significant differences before and after the treatment of extract and to be compared the difference between four groups with Dunnet T3 test to determine the difference between each group. Based on the statistical test results with Dunnet T3, it showed a significant difference between the fours study groups, except between treatment groups I and II. This might be due to the effectiveness of the two doses equally so that by doubling the dose, it did not give a doubling effect on decreasing spermatids. The provision of beluntas leaf extract can cause a decrease in the number of spermatid cells in mice. The average spermatid cells number decreased with increasing beluntas leaf extract dose. The best dose for reducing the number of spermatids in this study was the dose in the treatment group III that was 5.6 mg/g body weight.

scholarly journals The Effect of Training Mothers on Modifying the Meal Boxes of their Preschoolers with Vegetables and Fruits in the Sub-district of Tangerang, Tangerang City, Banten Province

2019 ◽  
Author(s):  
Ahmad Faridi ◽  
Tifani Larasati ◽  
Asri Werdhasari ◽  
Kenti Friskarini
Keyword(s):  
Treatment Group ◽  
Fruits And Vegetables ◽  
Statistical Tests ◽  
Food Composition ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Significant Difference ◽  
Before And After ◽  
Vegetables And Fruits

Background: Preschooler is a group who is at risk of having low-interest in consuming fruits and vegetables. The low-consumption of vegetables in children is influenced by the mother's lack of knowledge and incomplete composition of food. One way to improve vegetables and fruits consumption was through training in modifying meal box. Objectives: This study purposed at determining the effect of Modifying Meal Box Training to Increase Vegetables and Fruits Consumption for Preschool Children. Method: This study used a quasi-experimental research method with pre-test and post-test control group design.  Involving 40 mothers who took their children to school, this study conducted in the Pengayoman Kindergarten and Trisula Kindergarten in Tangerang City. The results of statistical tests showed that there were differences in knowledge before and after the intervention in the control group and the treatment group. Results: However, after the intervention there was no significant difference in knowledge in the control group and the treatment group with p value of 0.770. There were differences in food composition and consuming vegetables and fruits before and after the intervention in the treatment group. On the other hand, there were no differences in consuming vegetables and fruits consumption before and after intervention in the control group. After the intervention had been given, there was no significant difference in knowledge in the control group and the treatment group with p value of 0.270. Conclusions: Nevertheless, there are differences in the level of knowledge in the control group and the treatment group.  

scholarly journals Perbandingan Reaksi Zat Besi Terhadap Teh Hitam dan Teh Hijau Secara In Vitro dengan Menggunakan Spektrofotometer Uv-Vis

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
Husnil Wardiyah ◽  
Yustini Alioes ◽  
Dian Pertiwi
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Statistical Tests ◽  
Black Tea ◽  
Solution Concentration ◽  
T Test ◽  
Deficiency Anemia ◽  
Control Group ◽  
Significant Difference

AbstrakSalah satu permasalahan gizi yang dihadapi Indonesia adalah anemia defisiensi besi. Defesiensi besi ini dapat disebabkan oleh asupan dan serapan yang tidak adekuat, seperti kebiasaan mengonsumsi zat yang dapat menghambat penyerapan zat besi seperti minum teh pada saat makan. Hambatan penyerapan ini disebabkan oleh polifenol yang terkandung di dalam teh, terutama tanin. Penelitian ini dilakukan pada teh hitam dan teh hijau yang banyak dikonsumsi masyarakat. Larutan teh hitam dan teh hijau dijadikan sebagai kontrol, kemudian diberikan perlakuan dengan meneteskan FeCl3 1% sebanyak lima tetes. Larutan tersebut dibaca besar absorbannya dengan spektrofotometer UV-Vis. Prosedur ini dilakukan dengan pengulangan sebanyak lima kali. Data hasil penelitian diolah dengan menggunakan independent sample t test untuk melihat perbedaan rata-rata pada dua kelompok sampel tersebut. Dari hasil penelitian didapatkan rata-rata besar absorban teh hitam kontrol 0,539 dan setelah diteteskan zat besi 0,30640. Absorban teh hijau kontrol 0,961 dan setelah diteteskan zat besi 0,65020. Hal ini berarti bahwa terjadi penurunan konsentrasi larutan tersebut. Penurunan absorban pada kontrol teh hitam dengan perlakuan adalah 43,15%, sedangkan pada teh hijau adalah 32,34%. Berdasarkan uji statistik, disimpulkan bahwa terdapat perbedaan bermakna antara absorbansi teh hitam dan teh hijau.Kata kunci: Teh hitam, teh hijau, zat besi, absorban.AbstractOne of the nutritional problems faced by Indonesia is iron deficiency anemia. It is caused by inadequate intake and absorption. One of the causes of this inadequate absorption is eating habit to consume substances that can inhibit iron absorption like drinking tea while eating. This is caused by tea polyphenol compounds, especially tannins. Black tea and green tea were observed in this research since these are widely consumed by public. Solution of black tea and green tea were used as control and they were treated by giving five drops of 1% FeCl3. Absorbance of those solutions was read by using spectrophotometer UV-Vis. This procedure was done with five times repetition. The result of this research was processed by using independent sample t test to discern mean of two groups samples. The result showed that the absorbance mean of black tea control is 0.539 and the absorbance mean of black tea that has dripped by iron is 0.30640. As for the green tea control absorbance is 0.961 and absorbance mean after iron sheds is 0.65020. It means that there is a dilution and reduction of solution concentration. Based on these figures, it was concluded that the concentration of black tea with iron sheds is smaller than the concentration of green tea with iron sheds. Nevertheless, there is a greater reduction in black tea compare with green tea in the absorbance reduction. Absorbance reduction from black tea control group to treatment group is 43.15%, while absorbance reduction from green tea control group to treatment group is 32.34%. Based on statistical tests, it was concluded that there is a significant difference between the absorbance of black tea and green tea.Keywords: Black tea, green tea, iron, absorbance.

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