scholarly journals The effect of varied NAA concentration agains growth and flavonoid content of Gynura procumbens (Lour) Merr. callus

2004 ◽  
Vol 2 (2) ◽  
pp. 69-74
Author(s):  
ARIF HARDIYANTO ◽  
SOLICHATUN SOLICHATUN ◽  
WIDYA MUDYANTINI
Keyword(s):  
Treatment Phase ◽  
Dry Weight ◽  
Callus Growth ◽  
Cancer Drug ◽  
Naphthalene Acetic Acid ◽  
Second Phase ◽  
Flavonoid Content ◽  
Two Phase ◽  
Concentration Variation

The aim of this research is to study the influence of naphthalene acetic acid (NAA) concentration variation in the callus growth and flavonoid content of daun dewa [Gynura procumbens (Lour) Merr.]. The research framework relied on the benefit of daun dewa as anti cancer drug. This matter enabled by its compound cytotoxic from compound flavonoid contained. Culture in vitro technique serves the purpose of medium to produce the secondary metabolism. Addition of NAA as treatment for the induction of callus will promote cell enlargement and the growth of callus so that callus which later will be analysed by its flavonoid content. This research was conducted with two phase. First phase represented the phase of antecedent attempt in the form of callus induction to get the good callus. Addition of NAA and kinetin conducted to promote the cell proliferation. The parameter of this phase is texture, colour, and moment of callus appearance. Second phase is the form of NAA concentration variation treatment phase. At this research, it conducted with the random device complete with one factor that is 5 concentration NAA is 0 mg/L; 0,5 mg/L; 1,0 mg/L; 1,5 mg/L and 2,0 mg/L. The parameter of this phase is wet weight of the callus, dry weight of the callus, texture of the callus, colour of the callus, and flavonoid content. The result of this research indicated that the addition NAA does not influence on callus growth and the flavonoid content of callus daun dewa.

scholarly journals The effect of DL-tryptophan on callus growth and alkaloid-reserpin production of pule pandak [Rauvolfia serpentina (L.) Bentham ex Kurz] in vitro

2005 ◽  
Vol 3 (2) ◽  
pp. 52-56 ◽  
Author(s):  
HENI ARYATI ◽  
ENDANG ANGGARWULAN ◽  
SOLICHATUN SOLICHATUN
Keyword(s):  
Amino Acid ◽  
Quantitative Data ◽  
Callus Formation ◽  
Leaf Explants ◽  
Treatment Phase ◽  
Optimum Concentration ◽  
Callus Growth ◽  
Second Phase ◽  
Rauvolfia Serpentina

The purposes of this research were to study the effect of amino acid DL-tryptophan at various concentrations on culture callus growth and production of alkaloid-reserpine of Rauvolfia serpentina and to determine optimum concentration of DL-tryptophan to yield maximum alkaloid reserpine of the callus. This research was consisted of three phases. First phase was to determine compatible sterilan for R. serpentina leaf explants. Second phase was to initiate/ to induce callus formation from the explants. Third phase was treatment phase to know the influence of DL-tryptophan addition on growth and alkaloid-reserpine production of the callus. Experimental design that used was Completely Randomized Design (CRD). The treatment was concentration of the amino acid DL- tryptophan with three levels concentration three restating for each level. The levels were 0 mg/L, 10 mg/L and 20 mg/L. Obtained data were analyzed quantitatively and qualitatively. Quantitative data were wet weight, dry weight, growth rate and alkaloid content of the callus at each level of concentration. Qualitative data have been measured were compatible sterilant test and callus morphology (color and texture). Quantitative data then have been analyzed by analysis of variance (ANOVA) and continued with DMRT test at level 95%. The result indicated that addition of DL- tryptophan precursor had an effect on the reduction of the callus growth of R. serpentina in media MS in vitro. Addition of DL-tryptophan precursor to produce of alkaloid-reserpine of the callus of R. serpentina in media MS in vitro and optimum concentration of DL-tryptophan precursor that must be added to yield of alkaloid-reserpine could not be determined yet, it caused by the alkaloidreserpine was not detected yet by the thin layer chromatography (TLC).

scholarly journals Response of In Vitro Strawberry to Silver Nitrate (AgNO3)

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 747-751 ◽  
Author(s):  
Yonghua Qin ◽  
Shanglong Zhang ◽  
Lingxiao Zhang ◽  
Daoyu Zhu ◽  
Asghar Syed
Keyword(s):  
Acetic Acid ◽  
Silver Nitrate ◽  
Shoot Regeneration ◽  
Soluble Protein ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Antioxidant Enzyme Activities ◽  
Tetrazolium Chloride ◽  
Regeneration Efficiency

Response of Toyonoka strawberry to AgNO3 was studied. Types and combinations of plant growth regulators had significant effects on shoot regeneration efficiency. Explants cultured for 10 days in shoot regeneration medium in the presence of AgNO3 not only enhanced shoot regeneration efficiency but also expedited the initiation of adventitious buds. Highest regeneration (87.38%) and number of shoots per explant (11.67) were achieved in shoot regeneration media containing 1.5 mg·L–1 TDZ, 0.4 mg·L–1 IBA and 1.0 mg·L–1 AgNO3. Half-strength MS containing 1.0 mg·L–1 AgNO3 was an optimum medium for rooting. AgNO3 advanced root emergence and increased percent rooting, root length, dry weight and activity. Lower concentrations of AgNO3 inhibited ethylene production and promoted shoot regeneration and growth. It had a significant stimulatory effect on chlorophyll, soluble protein contents and antioxidant enzyme activities. Chlorophyll and soluble protein contents, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were increased in the presence of AgNO3 and reached maximum at 1.0 mg·L–1 AgNO3. Root water content, superoxide free radicals (O2.-), malondialdehyde (MDA) content, proline accumulation and IAA-oxidase activity in leaves were increased while (IAA) content was decreased in the presence of AgNO3. Chemical names used: indole-3-butyric acid (IBA); silver nitrate (AgNO3); thidiazuron (TDZ); N6-benzyladenine (BA); 2,4-dichlorophenoxy acetic acid (2,4-D); indole-3-acetic acid (IAA); α-naphthalene acetic acid (NAA); gibberellic acid (GA3); bovine serum albumin (BSA); 2,3,5-triphenyl-2H-tetrazolium chloride (TTC).

scholarly journals Scyphocephalione a Isolated from the Stem Bark of Scyphocephalium Ochocoa (Myristicaceae) Attenuate Acute and Chronic Pains Through the Antiinflammatory Activity

2022 ◽  
Author(s):  
Marius Mbiantcha ◽  
Raymond Tchouya Guy Feuya ◽  
William Nana Yousseu ◽  
Donatien Albert Atsamo ◽  
Hibrahim Foundikou ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Protein Denaturation ◽  
Cancer Drug ◽  
Cytotoxic Activities ◽  
Second Phase ◽  
Peripheral Neuropathic Pain ◽  
Anti Cancer ◽  
Anti Inflammatory

Abstract Abstract In the treatment of cancer, patients that receive anti-cancer drugs such as Vincristine develop peripheral neuropathic pain. Scyphocephalione A is a new bioactive compound isolated from Scyphocephalium ochocoa (Myristicaceae), a medicinal plant traditionally used in African countries. Recently, an in vitro study has shown its anti-inflammatory and cytotoxic activities on MCF-7 cell line of mammary carcinoma. The purpose of the present study was to assess the in vitro anti-inflammatory and in vivo anti-nociceptive activities of Scyphocephalione A. In vitro tests were carried out on cyclooxygenase and 5-lipoxygenase activities, and on protein denaturation; while in vivo tests were performed on acute and chronic pain models. It was noticed that, Scyphocephalione A (1000 µg/ml), inhibits proteins denaturation, cyclooxygenase and 5-lipoxygenase activities respectively by 74.21%, 75.80% and 64.43%. The dose 50 mg/kg of Scyphocephalione A, inhibits acetic acid (63.43%, p<0.001) and formalin (42.12% (p<0.001) within first phase and 67.53% (p<0.001) within second phase)-induced pains. At the same dose, Scyphocephalione A significantly inhibited mechanical and heat hyperalgesia, as well as cold allodynia induced by vincristine. In addition, the compound restored haematological, biochemical and oxidative stress parameters which were altered following Vincristine administration. These results suggest that Scyphocephalione A is endowed with anti-inflammatory potential and antinociceptive properties. Therefore, Scyphocephalione A can be classified as a promising molecule for the management of peripheral neuropathic pain triggered by anti-cancer drug.

Sampling oak foliage by the randomized-branch method

1977 ◽  
Vol 7 (2) ◽  
pp. 295-298 ◽  
Author(s):  
Harry T. Valentine ◽  
Susan J. Hilton
Keyword(s):  
Surface Area ◽  
Dry Weight ◽  
Total Count ◽  
Second Phase ◽  
Two Phase ◽  
Tree Crowns ◽  
Quercus Spp ◽  
Phase Sample

Foliage in mature Quercus spp. crowns was sampled by using a two-phase procedure. The first phase provided estimates of the total count of leaf clusters in the tree crowns. The second-phase sample provided information about the clusters such as dry weight, surface area, and leaf count.

scholarly journals Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro

2019 ◽  
Vol 7 (1) ◽  
pp. 16
Author(s):  
Ujang Siron ◽  
Noertjahyani Noertjahyani ◽  
Yana Taryana ◽  
Romiyadi Romiyadi
Keyword(s):  
Research Method ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Medium Culture

The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium  spectabile  orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1.  The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves.  Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets.  BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture 

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

2018 ◽  
Vol 11 (1) ◽  
pp. 322
Author(s):  
Sathvika Chintalapani ◽  
Swathi M. S. ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ether Extract ◽  
Sesuvium Portulacastrum ◽  
Flavonoid Content ◽  
Diethyl Ether Extract ◽  
Phenolic And Flavonoid

  Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays.Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

scholarly journals The effect of gamma radiation on callus growth and plantlet regeneration of four tomato hybrid Lycopersicon esculentum mill In vitro

2009 ◽  
Vol 3 (1) ◽  
pp. 56-62
Author(s):  
AssRana A.H. Al-roomia
Keyword(s):  
Lycopersicon Esculentum ◽  
Gamma Radiation ◽  
Dry Weight ◽  
Plantlet Regeneration ◽  
Callus Growth ◽  
Control Treatment ◽  
Ms Medium ◽  
Lycopersicon Esculentum Mill

Calluses were induced from four tomato hybrid (Davista, superDavista, Muntcarloo,Hymar). On MS medium supplemented with 2, 4-D Auxin at concentration of 4 mg/L.The Callus were divided into two groups the first one was affected with gammaradiation at the doses (0,2,4,6,8)Gray and re cultured on the same mentioned medium.The second group was also affected with gamma radiation with the same dosesmentioned above and re- cultured on MS media supplemented with 0.5 mg/L Kinetinfor regeneration. Fresh and dry weight was measured after 8 weeks. The number andlength of shoots were measured as indicators after 6 weeks. Results revealed that thedose 2 Gray was superior to give highest rate of fresh and dry weight reaching 238.5and 43.1 mg respectively and the hybrid super Davista was superior in giving thehighest fresh and dry weight reaching 231.6 and 30.2 mg respectively. Moreover theresults revealed that control treatment was superior in giving the highest rate innumber and length of plantlets. The 2 Gray treatment showed a significant effect onthe rate and length of plantlets. In addition, there were significant differences amongthe cultivars in most characters mentioned above.

scholarly journals Micropropagation of Eight Moroccan and French Olive Cultivars

HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 193-196 ◽  
Author(s):  
Saïda Sghir ◽  
Philippe Chatelet ◽  
Noureddine Ouazzani ◽  
Françoise Dosba ◽  
Ilham Belkoura
Keyword(s):  
Zeatin Riboside ◽  
Lag Phase ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Multiplication Rate ◽  
Two Phase ◽  
Best Response ◽  
Olive Cultivars ◽  
In Vitro Establishment

The responses of several Moroccan and French olive (Olea europaea L.) cultivars to various strategies for in vitro establishment and culture were compared. A cultivar effect was clearly observed with `Haouzia' cultivar being more readily multiplied. ZR produced the best response in all the cultivars studied, in particular when considering the time elapsed between explant inoculation and budbreak for 50% of the explants (lag phase), growth of the primary shoot and the multiplication rate. Treatments with BA alone or combined with NAA increased the number of axillary buds and internodes without improving their growth. Root induction with IBA in the dark using a two-phase scheme resulted in the best rooting rate in shoots obtained in vitro, and this for all cultivars. Chemical names used: 6-benzyladenine (BA), indole-3-butyric acid (IBA), alpha-naphthalene acetic acid (NAA), zeatin riboside (ZR).

scholarly journals Hyperhydricity and Flavonoid Content of Scutellaria Species In Vitro on Polyester-supported Liquid Culture Systems

HortScience ◽  
2010 ◽  
Vol 45 (11) ◽  
pp. 1723-1728 ◽  
Author(s):  
Ayse Tascan ◽  
Jeff Adelberg ◽  
Mevlut Tascan ◽  
Agnes Rimando ◽  
Nirmal Joshee ◽  
...  
Keyword(s):  
Time Course ◽  
Liquid Culture ◽  
Dry Weight ◽  
Dry Mass ◽  
Tissue Growth ◽  
Flavonoid Content ◽  
Physical Environments ◽  
Scutellaria Lateriflora ◽  
Reduced Water

Three Scutellaria species (Scutellaria lateriflora, S. costaricana, and S. baicalensis) were grown in different in vitro physical environments: agar, liquid culture, and liquid culture with fiber-supported paper (with initial media volumes of 20 mL and 30 mL). During an 8-week time course, tissue growth was assessed for each species by fresh weight (FW), dry weight (DW), percent DW, and multiplication ratio. Water use and hyperhydricity were also compared. Scutellaria lateriflora plantlets grown in liquid were hyperhydric despite the greatest accumulation of dry mass, but multiplication diminished with time as plants became hyperhydric. In contrast, S. costaricana and S. baicalensis plantlets had higher FW and DW on agar. With all Scutellaria species tested, plantlets grown on agar or fiber-supported paper were not hyperhydric, and fiber-supported paper with 20 mL initial volume yielded plants with the greatest percent DW. The lowered hyperhydricity was related to reduced water uptake. The flavonoids baicalin, baicalein, and wogonin were quantified in plants grown on fiber-supported paper culture. The baicalin concentrations in in vitro cultured S. lateriflora shoots was comparable to those of field-grown plants. The in vitro method presented a unique opportunity to enhance baicalein content and produce wogonin-rich roots. S. costaricana plantlets in vitro showed high levels of the three flavonoids compared with S. baicalensis and S. lateriflora. Growing non-hyperhydric tissues on fiber-supported paper, in vitro, allowed the clonal propagation of Scutellaria species with increased flavonoid content to proceed in a simple, controlled environment.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

2018 ◽  
Vol 11 (1) ◽  
pp. 322
Author(s):  
Sathvika Chintalapani ◽  
Swathi M. S. ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ether Extract ◽  
Sesuvium Portulacastrum ◽  
Flavonoid Content ◽  
Diethyl Ether Extract ◽  
Phenolic And Flavonoid

  Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays.Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

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