scholarly journals Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Ratna Setyaningsih ◽  
Ari Susilowati ◽  
Dina Prasetyani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Its Region ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Bacterial Cells ◽  
Methicillin Resistant

Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl acetate extract of matoa’s endophytic fungi to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also identify isolates having high antibacterial activity. Initial test on antibacterial activity was conducted by using paper disc diffusion, while advanced test was conducted by determining minimum inhibitory concentration (MIC). The fungi were identified in morphological and molecular manner with amplification and sequencing of ITS region. Bacterial cells exposed to the extract were observed by using scanning electron microscope (SEM). Isolate AM72 was found out to have the highest antibacterial activity compared to other 12 isolates tested, with inhibition zone diameter of 31.57 mm to S. aureus and 22.87 mm to MRSA. Isolate AM72 was identified as Lasiodiplodia theobromae. Isolate BMB identified as Aspergillus oryzae produced inhibition zone with 21.96 mm diameter to S. aureus and 21.50 mm diameter to MRSA. Advanced test to isolate AM72 gave the same MIC results to S. aureus and MRSA of 1.56%. Antibacterial compound produced by isolate AM72 were found out to be able to damage bacterial cell walls. Keywords: Antibacterial, Endohpytic Fungi, Matoa, S. aureus, MRSA  

Antibacterial Activity of Thai Medicinal Plants against Methicillin-resistant Staphylococcus aureus

Fitoterapia ◽  
2009 ◽  
Vol 80 (2) ◽  
pp. 102-104 ◽  
Author(s):  
Mullika Traidej Chomnawang ◽  
Suvimol Surassmo ◽  
Karn Wongsariya ◽  
Nuntavan Bunyapraphatsara
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Thai Medicinal Plants

scholarly journals Aktivitas antibakteri isolat bakteri endofit daun pegagan (Centella asiatica) terhadap Staphylococcus aureus

2021 ◽  
Vol 10 (1) ◽  
pp. 14-29
Author(s):  
Sembodho Edi Kurniawan ◽  
Mahyarudin Mahyarudin ◽  
Ambar Rialita
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Bacteria ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Centella Asiatica ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Genus Bacillus ◽  
Antibacterial Compounds ◽  
Methicillin Resistant

ABSTRAKAncaman global pada kasus Methicillin Resistant Staphylococcus aureus (MRSA) membutuhkan alternatif penanganan dengan tanaman obat tradisional. Bakteri endofit pada tanaman pegagan (Centella asiatica) memiliki kemampuan menghasilkan senyawa metabolit sekunder bersifat antibakteri yang serupa dengan tanaman inangnya. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri isolat bakteri endofit daun pegagan (C. asiatica) terhadap S. aureus. Penelitian ini bersifat deskriptif yaitu isolat bakteri endofit daun pegagan (C. asiatica) diujikan dengan metode difusi cakram terhadap S. aureus. Isolat yang paling berpotensi memiliki aktivitas antibakteri dilakukan uji metabolit untuk mengetahui senyawa antibakteri yang dihasilkan. Identifikasi bakteri endofit berdasarkan morfologi koloni, morfologi sel, dan uji biokimia. Hasil penelitian menunjukkan 2 dari 37 isolat memiliki aktivitas terhadap S. aureus  dengan zona hambat sebesar 9,02 mm dan 15,9 mm. Isolat yang paling berpotensi memiliki aktivitas tertinggi yaitu isolat I2 dengan zona hambat sebesar 15,9 mm. Isolat I2 memiliki kemiripan dengan genus Bacillus dan kemampuan mengasilkan senyawa antibakteri seperti alkaloid, saponin dan terpenoid.  Kata kunci: antibakteri; bakteri endofit; Centella asiatica; Staphylococcus aureus   ABSTRACTAntibacterial activity of endophytic bacteria isolate from pegagan leaves (Centella asiatica) against Staphylococcus aureusThe global threat in the case of Methicillin Resistant Staphylococcus aureus (MRSA) requires alternative treatment using traditional medicinal plants. Endophytic bacteria found in Pegagan plants (Centella asiatica) have ability to produce secondary metabolites with antibacterial capabilities similar to their host plants. The purpose of this study is to determine the antibacterial activity of endophytic bacterial isolates of Pegagan (C. asiatica) against S. aureus. This study is a descriptive research where endophytic bacterial isolates of Pegagan leaves (C. asiatica) were tested with disk diffusion method against S. aureus. The most potential isolates with antibacterial activity were performed metabolites test to determine the antibacterial compounds produced. Endophytic bacteria identification based on colony morphology, cell morphology and biochemical tests. The results showed that 2 out of 37 isolates had activity against S. aureus with inhibition zone of 9,02 mm and 15.9 mm. The most potential isolate that has highest activity was I2 isolate with inhibition zone of 15.9 mm. Isolate I2 has similarities with the genus Bacillus and the ability to produce antibacterial compounds such as alkaloids, saponins and terpenoids. Key words: antibacterial; Centella asiatica; endophytic bacteria; Staphylococcus aureus

scholarly journals Phytochemical analysis, antibacterial activity of hydromethanol extracts from stems of Ximenia americana, Côte d’Ivoire species on methicillin-resistant Staphylococcus aureus

2021 ◽  
Vol 14 (9) ◽  
pp. 3429-3440
Author(s):  
Baudelaire Affi Kakou ◽  
Anoubile Benie ◽  
Alain Hugues N’Guessan ◽  
Konan K. Fernique ◽  
N.K. Guessennd ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Public Health Problem ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Methicillin Resistant ◽  
Ximenia Americana

The emergence of bacteria resistant to several families of antibiotics is nowadays a public health problem in the world. To overcome this, it appeared necessary to explore sources of active molecules from natural substances. Thus, the objective of this study was to carry out the phytochemical sorting of hydromethanol extracts from Ximenia americana stems and to evaluate their antibacterial activities on the in-vitro growth of methicillin-resistant Staphylococcus aureus. The phytochemical screening performed allowed us to identify saponins, sterols and polyterpenes, polyphenols, tannins and flavonoids. HPLC-MS/MS analysis lead to the identification of a variety of flavan-3ol, quercetin and derivatives. The study of antibacterial activity carried out on 5 multi-resistant clinical strains and on a reference strain by the Muller-Hinton agar medium diffusion and dilution method showed that the extracts were active on all the strains with MICs ranging from 6.25 to 100 mg and MBCs ranging from 12.5 to 100 mg. The antibacterial potential of these extracts highlighted in this study could make this plant a candidate for in-depth investigations that could lead to the discovery of new antibacterial molecules. L’apparition de bactéries résistantes à plusieurs familles d’antibiotiques constitue, de nos jours, un problème de santé publique dans le monde. Pour y remédier, l’exploration de sources de molécules actives à partir des substances naturelles s’est avérée nécessaire. Ainsi, l’objectif de cette étude était de réaliser le tri phytochimique des extraits hydrométhanoliques de tiges de Ximenia americana et d’évaluer leurs activités antibactériennes sur la croissance in-vitro des Staphylococcus aureus résistant à la méticilline. Le screening phytochimique réalisé a permis d’identifier des saponines, des stérols et polyterpènes, des polyphénols, des tanins et des flavonoïdes. L’analyse à la HPLC-MS/MS a permis d’identifier une variété de flavan-3ol, de la quercétine et dérivées. L’étude de l’activité antibactérienne réalisée sur 5 souches cliniques multirésistantes et sur une souche de référence par la méthode de diffusion et de dilution en milieu gélosé Muller-Hinton a montré que les extraits étaient actifs sur toutes les souches avec des CMI variant de 6,25 à 100 mg et des CMB variant de 12,5 à 100 mg. Le potentiel antibactérien de ces extraits mis en évidence dans cette étude pourrait faire de cette plante une candidate à des investigations approfondies pouvant aboutir à la découverte de nouvelles molécules antibactériennes.

scholarly journals Chemical Profiling, Antioxidant and Antibacterial Activities of Essential Oil From Englerastrum gracillimum Th. C. E. Fries Growing in Niger

2021 ◽  
Vol 16 (3) ◽  
pp. 1934578X2110024
Author(s):  
Bala Namata Abba ◽  
Amadou Tidjani Ilagouma ◽  
Issoufou Amadou ◽  
Abderrahmane Romane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Antioxidant Power ◽  
Methicillin Resistant ◽  
Agar Disc

In the present study, the chemical composition, antioxidant and antibacterial activities of the essential oil obtained by hydrodistillation of the aerial parts of Englerastrum gracillimum Th. C. E. Fries growing in Niger were investigated. Gas chromatography-mass spectrometry (GC–MS) analysis of the essential oil resulted in the identification of 42 compounds representing 97.9% of the total oil constituents. The major compounds of the essential oil were: α-humulene (30.5%), followed by cubenol (19.8%), γ-muurolene (14.0%), ( E)-β-caryophyllene (5.8%), β-gurjunene (5.2%), and curzerene (4.9%). The antioxidant activity of the essential oil was determined by using the free radical-scavenging activity (2,2 diphenyl-1-picrylhydrazyl: DPPH˙) and ferric reducing antioxidant power assays. The essential oil showed good antioxidant potential with both methods. The antibacterial activity was evaluated against multi-resistant Acinetobacter baumannii P1483, extended-spectrum β-lactamase (ESBL) -Escherichia coli Bu8566, Salmonella spp. H1548, Proteus mirabilis Bu190 , Enterobacter cloacae Bu147, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), methicillin-resistant Staphylococcus aureus P1123, Enterococcus faecium H3434, and Staphylococcus aureus (ATCC 25923) using the agar disc diffusion and microdilution methods. The essential oil exhibited good antibacterial activity. The highest antibacterial effect was observed against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus P1123 with minimal inhibitory concentration (MIC) values of 0.03 mg/mL and 0.06 mg/mL, respectively. These data are of scientific importance for the valorization of aromatic and medicinal plants of Niger, especially E. gracillimum. This study reports for the first time the antioxidant and antibacterial activities of this essential oil.

Antibacterial Activity of Ecklonia cava Against Methicillin-Resistant Staphylococcus aureus and Salmonella spp.

2010 ◽  
Vol 7 (4) ◽  
pp. 435-441 ◽  
Author(s):  
Jang-Gi Choi ◽  
Ok-Hwa Kang ◽  
Obiang-Obounou Brice ◽  
Young-Seob Lee ◽  
Hee-Sung Chae ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ecklonia Cava ◽  
Salmonella Spp ◽  
Methicillin Resistant
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