Cardioprotective Effects of Radix on the Pressure Overloaded Heart Failure Model by Transverse Aortic Constriction-induced Mice

2016 ◽  
Vol 37 (2) ◽  
pp. 23-35
Author(s):  
Sae-Won Kim ◽  
Woo-Seok Jang ◽  
Kyung-Min Baek
Keyword(s):  
Heart Failure ◽  
Failure Model ◽  
Transverse Aortic Constriction ◽  
Aortic Constriction ◽  
Cardioprotective Effects ◽  
Heart Failure Model

scholarly journals Molecular Mechanism of HSF1-Upregulated ALDH2 by PKC in Ameliorating Pressure Overload-Induced Heart Failure in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Endong Ji ◽  
Tiantian Jiao ◽  
Yunli Shen ◽  
Yunjia Xu ◽  
Yuanqing Sun ◽  
...  
Keyword(s):  
Heart Failure ◽  
Myocardial Hypertrophy ◽  
Pressure Overload ◽  
Mechanical Stretch ◽  
Compensatory Hypertrophy ◽  
Failure Model ◽  
Transverse Aortic Constriction ◽  
Aortic Constriction ◽  
Heart Failure Model

Evidences abound that HSF1 and ALDH2 are of cardioprotective effect, yet there is still no report on whether HSF1 can regulate ALDH2 to delay the occurrence of heart failure. We first established the pressure overload-induced heart failure model of mice by transverse aortic constriction (TAC) and discovered that, in the forming period of heart failure, changes of HSF1 and ALDH2 expression recorded the consistent trend. When HSF1 was upregulated/downregulated to delay/promote the occurrence of heart failure, PKC and ALDH2 also showed increased/decreased expression. And when ALDH2 was upregulated/downregulated, the role of HSF1 in delaying the occurrence of heart failure strengthened/weakened. Next, we used mechanical stretch to establish a pressure-stimulated myocardial hypertrophy model and discovered an increased expression of both HSF1 and ALDH2. When HSF1 was upregulated/downregulated to increase/decrease the expression of myocardial hypertrophy gene beta-MHC, PKC and ALDH2 recorded an increased/decreased expression. When an inhibitor was used to downregulate the expression of PKC in cardiomyocytes, we found that the role of HSF1 in upregulating ALDH2 beta-MHC weakened. These findings suggest that HSF1 can upregulate the expression of ALDH2 via PKC to promote pressure-stimulated myocardial compensatory hypertrophy, which is an important molecular pathway for HSF1 to ameliorate heart failure.

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