Production of Short-Chain Carboxylic Acids by Co-Digestion of Swine Manure and Corn Silage: Effect of Carbon-Nitrogen Ratio

2020 ◽  
Vol 63 (2) ◽  
pp. 445-454 ◽  
Author(s):  
Qitao Cao ◽  
Wanqin Zhang ◽  
Yunhao Zheng ◽  
Tianjing Lian ◽  
Hongmin Dong
Keyword(s):  
Lactic Acid ◽  
Carboxylic Acids ◽  
Agricultural Waste ◽  
Swine Manure ◽  
Corn Silage ◽  
Short Chain ◽  
Fuel Production ◽  
Energy Value ◽  
Unit Energy ◽  
Acid Producing Bacteria

HighlightsThe maximum SCCA concentration was achieved at a C/N ratio of 25 with 18 days of fermentation.The highest VFAs and LA were produced at C/N ratios of 12.7 and 35, respectively, after 12 days of fermentation.The unit energy value of SCCAs for drop-in fuel production was 50.5% higher than that of methane.The dominant bacteria changed from Clostridium to Lactobacillus with increasing C/N ratio.Abstract. Advancing technologies to produce short-chain carboxylic acids (SCCAs) from agricultural waste are expected to be more economical and efficient. This study presented a new attempt to enhance SCCA production from co-digestion of swine manure and corn silage with the carbon-nitrogen (C/N) ratio adjusted from 12.7 to 50.2. Results showed that the maximum SCCA concentration of 26,214 ±2948 mg COD L-1 was achieved at a C/N ratio of 25 with 18 days of digestion, and the concentrations of lactic acid (LA) and volatile fatty acids (VFAs) were 8700 ±1060 mg COD L-1 and 17,514 ±1888 mg COD L-1, respectively. The LA content first increased and then slightly decreased with increasing C/N ratio, and the maximum LA concentration was 468.5 ±44.3 mg COD g-1 VSadded (VSadded means added fermentation material is calculated as volatile solids) at a C/N ratio of 35 at 12 days of digestion. VFAs showed an opposite trend, with a maximum VFA concentration of 601.1 ±42.4 mg COD g-1 VSadded found at a C/N ratio of 12.7 after 12 days of digestion. Potential energy analysis of the highest SCCA production showed that the unit energy value of SCCAs for drop-in fuel production was 50.5% higher than that of methane. Analysis of the microbial community showed that the dominant bacterial groups changed gradually from butyric acid-producing bacteria (Clostridium) to lactic acid-producing bacteria (Lactobacillus) as the C/N ratio increased from 12.7 to 50.2. Keywords: Anaerobic digestion, Corn silage, Lactic acid, Short-chain carboxylic acids, Swine manure.

Short chain carboxylic acids production and dynamicity of microbial communities from co-digestion of swine manure and corn silage

2021 ◽  
Vol 320 ◽  
pp. 124400
Author(s):  
Qitao Cao ◽  
Wanqin Zhang ◽  
Tianjing Lian ◽  
Shunli Wang ◽  
Hongmin Dong
Keyword(s):  
Microbial Communities ◽  
Carboxylic Acids ◽  
Swine Manure ◽  
Corn Silage ◽  
Short Chain

scholarly journals Short-Chain Carboxylic-Acid-Stimulated, PMN-Mediated Gingival Inflammation

1997 ◽  
Vol 8 (3) ◽  
pp. 269-290 ◽  
Author(s):  
Richard Niederman ◽  
Jie Zhang ◽  
Shelby Kashket
Keyword(s):  
Lactic Acid ◽  
Inflammatory Response ◽  
Carboxylic Acids ◽  
Propionic Acid ◽  
Biological Effects ◽  
Short Chain ◽  
Active Principle ◽  
Cytokine Release ◽  
Gingival Inflammation ◽  
Differentiating Agent

This communication reviews the effects of short-chain carboxylic acids on human cells of importance to the periodontium. The central hypothesis is that these acids can alter both cell function and gene expression, and thus contribute to the initiation and prolongation of gingival inflammation. Short-chain carboxylic acids [CH3-(CH2)x-COOH, x < 3] are metabolic intermediates with a broad range of apparently paradoxical biological effects. For example, lactic acid (CH3-CHOH-COOH), a 3-carbon alpha-hydroxy-substituted acid, is widely recognized for its cariogenicity. Lactic acid, however, also occurs in tropical fruits, and is the active ingredient in a variety of anti-wrinkle creams developed by dermatologists. In marked contrast, the unsubstituted 3-carbon propionic acid (CH3-CH 2-COOH) is used as a food preservative and is the active principle for one class of non-steroidal anti-inflammatory agents. Interestingly, the addition of one carbon to propionic acid dramatically changes the biological effects. The unsubstituted 4-carbon butyric acid (CH3-CH2-CH2-COOH) is used by hematologists as a de-differentiating agent for the treatment of sickle cell anemia, but by oncologists as a differentiating agent for cancer chemotherapy. Finally, acting either individually or in concert, these acids can increase vascular dilation. Clearly, these acids, while metabolically derived, have a number of very divergent activities which are cell-type-specific (Fig. 1). It may be telling that periodontal bacteria produce these acids in millimolar concentrations, and that these bacteria can be characterized by their acid production profiles. It is no less interesting that these acids occur in the gingival crevices of human subjects with severe periodontal disease at millimolar levels which are > 10-fold higher than those found in mildly diseased subjects, and are undetectable in healthy subjects. Further, when applied directly to healthy human gingiva, short-chain carboxylic acids stimulate a gingival inflammatory response and inflammatory cytokine release. At the cellular level, these acids inhibit proliferation of gingival epithelial and endothelial cells, and inhibit leukocyte apoptosis and function, but can stimulate leukocyte cytokine release. At the molecular level, these acids can stimulate neutrophil gene transcription, translation, and protein expression. Thus, the likelihood is high that these acids, in addition to their cariogenic activity, can promote and prolong gingival inflammation. Our challenge will be to identify the cell or cells of the periodontium which respond to short-chain carboxylic acids, to delineate their responses and the molecular mechanism(s) of these effects, and to categorize the aspects of the inflammatory components which damage and those which protect the host. With this information, it may be possible to begin to rationally identify and test pharmaceutical agents which diminish the harmful aspects, while enhancing the beneficial components, of the inflammatory response.

scholarly journals Commercial microbial inoculants of lactic acid-producing bacteria on fermentative characteristics and aerobic stability of corn silage

2014 ◽  
Vol 98 (1) ◽  
Author(s):  
Abner A. Rodríguez ◽  
Viviana Rivera ◽  
Luis C. Solórzano ◽  
Paul F. Randel
Keyword(s):  
Lactic Acid ◽  
Corn Silage ◽  
Control Treatment ◽  
Tropical Maize ◽  
Microbial Inoculants ◽  
Average Percentage ◽  
Average Value ◽  
Average Temperature ◽  
Aerobic Stability ◽  
Acid Producing Bacteria

This study evaluated two treatments based on addition of two commercial microbial inoculants of lactic acid-producing bacteria (LAPB), one of the homolactic type (HOT) and the other a combination of homolatic with heterolactic bacteria (HHT), plus a control treatment without inoculation (CNT), on the fermentative characteristics of tropical maize in micro-silos and aerobic stability (AS) of the resulting silage. The fermentative characteristics were determined in triplicate samples taken at various lengths of fermentation (15, 30, 58 and 90 d), which were analyzed for pH, concentration of organic acids and NHL. To evaluate AS, changes in pH and temperature of the silage were monitored during five days of exposure to air. No significant differences were found among treatments in any of the fermentation characteristics studied. HOT resulted in the numerically lowest average pH (4.23) and the highest average percentage content of lactic acid (2.18), followed by HHT (4.28 and 2.11) and CNT (4.32 and 1.94), respectively. Also, regarding indicators of AS there were no significant differences among treatments, but numerically the average pH of exposed HOT silage was lower (5.30 vs 5.43 the average value of both HHT and CNT) and there was a trend (P <0.15) toward lower average temperature (°C ) in favor of HHT (29.61 vs. 30.26 and 30.28 for HOT and CNT silages). In general, the use of LAPB inoculants in tropical maize silage did not result in large differences relative to non-inoculated silage in the variables under study regarding fermentation characteristics and AS.

scholarly journals THE RELATION OF LACTIC ACID BACTERIA TO CORN SILAGE

1921 ◽  
Vol 46 (2) ◽  
pp. 319-327 ◽  
Author(s):  
E.B. Fred ◽  
W.H. Peterson ◽  
J.A. Anderson
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Corn Silage

Combined use of lactic-acid-producing bacteria as probiotics and rotavirus vaccine candidates expressing virus-specific proteins

2021 ◽  
Vol 166 (4) ◽  
pp. 995-1006
Author(s):  
Atefeh Afchangi ◽  
Tayebeh Latifi ◽  
Somayeh Jalilvand ◽  
Sayed Mahdi Marashi ◽  
Zabihollah Shoja
Keyword(s):  
Lactic Acid ◽  
Rotavirus Vaccine ◽  
Vaccine Candidates ◽  
Combined Use ◽  
Specific Proteins ◽  
Acid Producing Bacteria

Monitoring the short-chain carboxylic acids produced during the storage of different fatty composition biodiesels and their binary blends using ion chromatography

Fuel ◽  
2021 ◽  
Vol 289 ◽  
pp. 119943
Author(s):  
Patrícia T. Souza ◽  
Willian L.G. Silva ◽  
Antonio J.A. Meirelles ◽  
Matthieu Tubino
Keyword(s):  
Carboxylic Acids ◽  
Ion Chromatography ◽  
Short Chain ◽  
Binary Blends
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