Comparative analysis of rice seed viability detection based on different spectral bands

2020 ◽  
Author(s):  
Yali Wang ◽  
Yankun Peng ◽  
Xin Qiao ◽  
Qibin Zhuang
Keyword(s):  
Comparative Analysis ◽  
Seed Viability ◽  
Rice Seed ◽  
Spectral Bands

scholarly journals DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm

2019 ◽  
Vol 116 (19) ◽  
pp. 9652-9657 ◽  
Author(s):  
M. Yvonne Kim ◽  
Akemi Ono ◽  
Stefan Scholten ◽  
Tetsu Kinoshita ◽  
Daniel Zilberman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Vegetative Cell ◽  
Seed Viability ◽  
Dna Demethylation ◽  
Central Cell ◽  
Dna Glycosylase ◽  
Rice Seed ◽  
Vegetative Cells ◽  
Active Dna Demethylation ◽  
Cell Genome

Epigenetic reprogramming is required for proper regulation of gene expression in eukaryotic organisms. In Arabidopsis, active DNA demethylation is crucial for seed viability, pollen function, and successful reproduction. The DEMETER (DME) DNA glycosylase initiates localized DNA demethylation in vegetative and central cells, so-called companion cells that are adjacent to sperm and egg gametes, respectively. In rice, the central cell genome displays local DNA hypomethylation, suggesting that active DNA demethylation also occurs in rice; however, the enzyme responsible for this process is unknown. One candidate is the rice REPRESSOR OF SILENCING1a (ROS1a) gene, which is related to DME and is essential for rice seed viability and pollen function. Here, we report genome-wide analyses of DNA methylation in wild-type and ros1a mutant sperm and vegetative cells. We find that the rice vegetative cell genome is locally hypomethylated compared with sperm by a process that requires ROS1a activity. We show that many ROS1a target sequences in the vegetative cell are hypomethylated in the rice central cell, suggesting that ROS1a also demethylates the central cell genome. Similar to Arabidopsis, we show that sperm non-CG methylation is indirectly promoted by DNA demethylation in the vegetative cell. These results reveal that DNA glycosylase-mediated DNA demethylation processes are conserved in Arabidopsis and rice, plant species that diverged 150 million years ago. Finally, although global non-CG methylation levels of sperm and egg differ, the maternal and paternal embryo genomes show similar non-CG methylation levels, suggesting that rice gamete genomes undergo dynamic DNA methylation reprogramming after cell fusion.

Effect of High Temperature Intermittent Drying on Rice Seed Viability and Vigor

2017 ◽  
Vol 13 (10) ◽  
Author(s):  
Pan Wang ◽  
Dong Li ◽  
Li-jun Wang ◽  
Benu Adhikari
Keyword(s):  
High Temperature ◽  
Ambient Temperature ◽  
Seed Viability ◽  
Seed Vigor ◽  
Drying Rate ◽  
Rice Seed ◽  
Rice Cultivars ◽  
Moisture Contents ◽  
Rice Seeds ◽  
Tempering Process

AbstractThis work aimed at determining whether high temperature intermittent drying followed by tempering at ambient temperature could preserve the seed viability and vigor.JaponicaandIndicarice seeds with 21.2 % and 22.6 % wet moisture contents (w.b.) were dried at 50 ºC and 60ºC for either 5, 10, 15 or 20 minutes, followed by tempering at 25ºC for 45 minutes. Each drying cycle was repeated until the rice seeds were dried to 12.0 % (w.b.). The drying rate was improved and the total in-dryer time was reduced in the intermittent drying when compared to continuous drying, due to the tempering process. The seed vigor was significantly reduced by intermittent drying at 60 °C with all exposure times, although the seed still kept the ability to germinate for both rice cultivars. The intermittent drying at 50 ºC for 5 minutes per drying cycle preserved the seed vigor ofJaponicarice well, while inIndicarice, the intermittent drying at 50 ºC up to 20 minutes could still be able to maintain the seed vigor.

scholarly journals Keefektifan Pelapisan Benih terhadap Peningkatan Mutu Benih Padi Selama Penyimpanan

2015 ◽  
Vol 34 (2) ◽  
pp. 145
Author(s):  
Ikrarwati Ikrarwati ◽  
Satriyas Ilyas ◽  
AmiyarsI Mustika Yukti
Keyword(s):  
Pathogenic Bacteria ◽  
Seed Viability ◽  
Seed Coating ◽  
Rice Seed ◽  
Clove Oil ◽  
Arabic Gum ◽  
Control Seed ◽  
Citronella Oil ◽  
Lemon Grass ◽  
Four Levels

Seed coating using biological pesticides such as clove oil and citronella oil, was expected to have the ability to control seed-borne pathogens on rice. The aim of the research was to determine the effectiveness of seed coating using clove oil and lemon grass oil against seed-borne fungi and bacteria on rice seed variety “Hipa 8” during storage. The experiment was conducted in Cimanggis, from February to September 2012, using completely randomized design with single factor, consisting of four levels: (1) clove oil 1% + chitosan 3%; (2) lemon grass oil 2% + carboxymethyl-cellulose 1%; (3) Synthetic pesticide (streptomycin sulphate 0.04% + benomyl 0.1%) + arabic gum 10%; and (4) control (without coating). Results showed that seedborne fungi pathogens on seed of Hipa 8 rice were detected at 6-month storage consisted of Fusarium sp., Curvularia sp., Alternaria sp., Cladosporium sp., Aspergillus sp. and Penicillium sp. The detected pathogenic bacteria were Xanthomonas oryzae pv. oryzae and X. campestris pv. oryzicola. Coating formula most compatible with the rice seed of HIPA 8 was chemical pesticide + 10% arabic gum, which suppressed fungal infection from 80% to 45% at the first month and from 90% to 70% at the fifth month, suppressed populations of Xoo + Xco from 7.6 x 108 cfu/g of seed to 5.86 x 105 cfu/g of seed at the first month and 7.0 x 106 cfu/g of seed to 1.4 x 104 cfu/g of seeds at the sixth month. The treatment caused the smallest decrease of seed viability compared to the other coating treatments.

scholarly journals Effect of simulated flooding during rice seed development and maturation on subsequent seed quality

2018 ◽  
Vol 28 (1) ◽  
pp. 72-81 ◽  
Author(s):  
Sujittra Tejakhod ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Seed Development ◽  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Rice Seed ◽  
Hermetic Storage ◽  
Dry Storage ◽  
Sprouted Seeds ◽  
Harvest Maturity

AbstractThe resilience of seed quality in rice (Oryza sativaL.) to flooding was investigated. Pot-grown plants of thejaponicacv. Gleva, theindicacv. IR64, and the introgressed line IR64-Sub1 were submerged in water, to simulate flooding, for 3‒5 days at different stages of seed development and maturation. Mean seed weight, pre-harvest sprouting, ability to germinate, and subsequent longevity in air-dry storage were assessed. Whereas seed quality in both IR64 and IR64-Sub1 was resilient to submergence, in Gleva the longer the duration of submergence and the later in development when plants were submerged the greater the pre-harvest sprouting. Thousand seed dry weight was reduced more by submergence in Gleva than IR64 or IR64-Sub1. At harvest maturity, few pre-harvest sprouted seeds were able to germinate upon rehydration after desiccation to 11‒12% moisture content. Seed longevity of the non-sprouted seed fraction in air-dry hermetic storage (40°C, 15% moisture content) was not affected greatly by submergence, but longevity of thejaponicarice was less than that of theindicarices due to the former's steeper seed survival curves. Longevity of the twoindicarices was predicted well by the seed viability equation and previously published estimates of viability constants for rice. The greater dormancy of IR64 and IR64-Sub1, compared with Gleva, enhanced resilience to pre-harvest sprouting and reduced thousand seed dry weight from plant submergence. There was little or no effect of plant submergence on subsequent air-dry storage longevity of non-sprouted seeds in any genotype.

scholarly journals Grain Quality of Rice (Oryza sativa L.) ‘Menthik Wangi’ of Organic Farming Yields

2021 ◽  
Vol 28 (2) ◽  
pp. 105
Author(s):  
Dwi Umi Siswanti ◽  
Maryani Maryani ◽  
Fitria Yulia Rachmawati ◽  
Aprida Niken ◽  
Rega Virgiyana Agustin ◽  
...  
Keyword(s):  
Organic Farming ◽  
Oryza Sativa L ◽  
Seed Viability ◽  
Fat Content ◽  
Growth Potential ◽  
Rice Grain ◽  
Rice Seed ◽  
Organic Rice ◽  
Organic Cultivation

Organic rice is believed to have better nutrition than conventional rice. Not all organic rice grain is consumed. Most of the organic cultivation yields will be used as seed. Viability test is one of the quality test of rice seed. Viability is seed vitality that can be shown as a physiological and biochemical phenomenon. Seed that have high vigor will achieve high productivity levels as well. The aims of this research was to analyze the quality of rice cultivar Menthik Wangi of organic farming yields. The quality of rice seeds was tested by measuring the viability of seed and crude fat content. The test results demonstrate the viability of the grain has a 100% germination, 100% growth potential maximum, 99.33%, simultaneity grew, 97.33% vigor index, T50 2.33, and seed moisture content 10.67%. The fat content was conducted by soxhlet method and analyzed by T-test. The results showed that organic rice has a higher fat content (0.53%) than non-organic rice (0.24%). It can be concluded that rice seed cultivar Menthik Wangi of organic farming has a higher viability and fat content than the non-organic rice grain.

scholarly journals Peningkatan Performa Viabilitas Benih Beberapa Varietas Padi (Oryza sativa L.) Dengan Organik Priming Ekstrak Tauge

2020 ◽  
Vol 16 (2) ◽  
pp. 309
Author(s):  
Nove Arisandi ◽  
Raihani Wahdah ◽  
Gusti Rusmayadi
Keyword(s):  
Oryza Sativa L ◽  
Block Design ◽  
Seed Viability ◽  
Rice Seed ◽  
Bean Sprout ◽  
Randomized Block Design ◽  
Interaction Factor ◽  
Seed Growth ◽  
Long Time ◽  
Biochemical Condition

Quality seed is one of the determining factors of successful agricultural cultivation. Seeds that have been stored for a long time will experience seed deterioration, which is characterized by a decrease in seed viability. Improving seed viability can be done by seed invigoration. One of the seed invigoration techniques is organic priming by using organic substances extracts that are rich in phytohormones such as auxin, cytokinin, and gibberellins. The purpose of Organic priming is to improve the seed physiological and biochemical condition that related to growing speed, growing simultaneity, and germination. Mung bean sprout extract can be used as an extract for organic priming because it is proven to contain phytohormones. This study aims to determine the interaction between varieties and concentrations of bean sprout extracts and the best combination of varieties and extract concentrations on the viability of rice seeds. The research was carried out in April - June 2020 at the Laboratory of Agricultura Biology, Agriculture Faculty, Lambung Mangkurat University. The experiment  was arranged based on Factorial Randomized Block Design (RBD) 4x6 with Three replications. The variables observed were seeds germination, simultaneity of seedlings growth and speed of seedlings growth. The results showed that the interaction factor between varieties and concentration had a very significant effect on increasing seed viability and the best combination was found in Baroma varieties with concentrations of 20 % and if concentrations higher or lower than 20 % performance of rice seed viability will decrease. The highest percentage of seed germination was found in Baroma varieties with a concentration of 20 % (v4k3), which is 82.67 % not significantly different from Baroma varieties with a concentration of 10 % (v4k2) and INPARA 3 varieties with a concentration of 20 % (v1k3). The highest percentage of simultaneity of seed growth was found in Baroma varieties with a concentration of 20 % (v4k3), which is 81.50 % not significantly different from Baroma varieties with a concentration of 10 % (v4k2), Mutant line No. 14 with a concentration of 20 % (v3k3), and INPARA 3 variety with a concentration of 20 % (v1k3), while the fastest percentage of seed growth rate is in Baroma with a concentration of 20 % (v4k3), which is 29.50% significantly different from all varieties and other bean sprout extract concentrations.

scholarly journals Deteksi Cepat Viabilitas Benih Padi Menggunakan Gelombang Near Infrared dan Model Jaringan Saraf Tiruan

2014 ◽  
Vol 33 (2) ◽  
pp. 77
Author(s):  
Jonni Firdaus ◽  
Rokhani Hasbullah ◽  
Usman Ahmad ◽  
M. Rahmad Suhartanto
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Soluble Protein ◽  
Input Data ◽  
Near Infrared ◽  
Seed Viability ◽  
Accelerated Aging ◽  
Rice Seed ◽  
Ann Models ◽  
Rice Seeds

Viability is an important component of seed quality, which could be detained by germinating the seeds. Currently testing the seed viability of rice takes a long time (5-14 days), so it becomes a limiting factor in the seed production process. An alternative method for rapid seed viability detection is using the Near Infrared (NIR) spectra and using artificial neural network (ANN) as a data processing system. This research was aimed to study the use of NIR spectra and ANN to predict the viability of rice seeds. NIR reflectance (1,000-2,500 nm) of a Ciherang rice seed samples (40 grams), was used as the input data to develop the ANN model. A total of 60 samples were subjected to accelerated aging to obtain various levels of germination. The development of ANN models was done through calibration and validation of NIR spectra to the viability parameters. As ANN input, NIR reflectance of seed sample was given pretreatment data such as normalization, first derivative, second derivative, standard normal variate (SNV) and principal component analysis (PCA). The results showed that longer accelerated aging caused a decrease in seed viability. This was also indicated by the decrease in soluble protein and an increase in free fatty acids. The intensity of the NIR absorbance spectra also showed the same in the absorption region of soluble protein and free fatty acids. The best ANN models to predict the germination was 10PC-5-3 ANN with the SNV NIR reflectance used as the input data. Coefisien correlation of the validation was 0.8947, the value of ratio performance deviation was 2.2359 and the standard error performance was 9.9233%. The use of NIR spectra and ANN was potentially useful to perdict the viability of rice seeds more rapidly.

scholarly journals O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ

2021 ◽  
Vol 36 (1) ◽  
pp. 28-40
Author(s):  
Jaquelini Garcia ◽  
Cileide Maria Medeiros Coelho
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Randomized Design ◽  
Physiological Quality ◽  
Drying System

O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ   JAQUELINI GARCIA1, CILEIDE MARIA MEDEIROS COELHO1   1Programa de Pós-graduação em Produção vegetal, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brasil, [email protected], [email protected]   RESUMO: O grau de umidade na colheita e o sistema de secagem podem ser determinantes na qualidade das sementes produzidas. Neste trabalho foi avaliado se o sistema de secagem e o grau de umidade na colheita interferem na qualidade fisiológica das sementes de arroz. Foi realizado em delineamento inteiramente casualizado em esquema fatorial triplo. Foi utilizada seis cultivares produzidas no Alto Vale do Itajaí/SC na safra 2016/17 com grau de umidade na colheita igual ou superior a 17% (19,30 - 17,30%) e inferior a 17% (16,40 - 15,03%) (base úmida). As sementes foram submetidas aos sistemas de secagem estacionário e intermitente. Realizou-se testes de germinação, viabilidade, vigor pelo envelhecimento acelerado e frio. Sementes colhidas com grau de umidade ≥ 17% apresentaram maior vigor. A cultivar foi um fator de grande relevância na sensibilidade ao dano térmico e mecânico. O sistema de secagem não afetou a viabilidade das sementes, com exceção da cultivar SCS122 Miura que foi sensível a dano térmico e mecânico. A secagem estacionária comprometeu a qualidade fisiológica. Conclui-se que a secagem intermitente e a colheita com grau de umidade igual ou superior a 17%, mantém a qualidade fisiológica das cultivares SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru e SCS121 CL.   Palavras-chaves: Secagem estacionária, secagem intermitente, qualidade fisiológica.   THE DEGREE OF MOISTURE IN THE HARVEST AND THE DRYING SYSTEM ARE DETERMINANT FOR THE RICE SEED VIGOR   ABSTRACT: The degree of moisture at harvest and the drying system can determine the quality of the seeds produced. In this work, it was evaluated whether the drying system and the degree of moisture at harvest affect the physiological quality of rice seeds. It was conducted in a completely randomized design in a triple factorial scheme. Six cultivars produced in Alto Vale do Itajaí/SC in the 2016/17 crop were used, with moisture content at harvest equal or greater than 17% (19.30 – 17.30%) and less than 17% (16,40 - 15,03%) (wet basis). Seeds were submitted to stationary and intermittent drying systems. Tests of germination, viability, vigor by accelerated aging and cold were performed. Seeds harvested with moisture content ≥ 17% showed greater vigor. The cultivar was a factor of great relevance in the sensitivity to thermal and mechanical damage. The drying system did not affect seed viability, except for the cultivar SCS122 Miura, which was sensitive to thermal and mechanical damage. Stationary drying compromised physiological quality. It was concluded that intermittent drying and harvesting with moisture content equal or greater than 17% maintain the physiological quality of the SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru and SCS121 CL cultivars.   Keywords: Stationary drying, intermittent drying, physiological quality.

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