3D-printed pneumatic microfluidic mixer for colorimetric detection of <i>Listeria monocytogenes</i>

2018 ◽  
Author(s):  
Ping Yao ◽  
Nathan Harris ◽  
Ronghui Wang ◽  
Xinge Xi ◽  
Jingyi Wang ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Colorimetric Detection ◽  
Microfluidic Mixer ◽  
3D Printed

3D-Printed Pneumatic Microfluidic Mixer for Colorimetric Detection of Listeria monocytogenes

2019 ◽  
Vol 62 (3) ◽  
pp. 841-850 ◽  
Author(s):  
Ping Yao ◽  
Ronghui Wang ◽  
Xinge Xi ◽  
Yanbin Li ◽  
Steve Tung
Keyword(s):  
Listeria Monocytogenes ◽  
3D Printing ◽  
Rapid Detection ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Single Step ◽  
Mixing Efficiency ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microfluidic Mixer ◽  
3D Printed

Abstract. 3D printing can significantly improve the current fabrication techniques for microfluidic devices due to its ability to create truly 3D structures in a single step. In this study, an active pneumatic microfluidic mixer was designed and fabricated using an extrusion-based 3D printer and used for rapid detection of . The printed material of the mixer is flexible, semi-transparent, and inexpensive. The fabrication time is significantly shorter than the traditional micromolding process. The printed mixer consists of two pneumatic air chambers and one mixing chamber designed for a fluidic sample size of 100 µL. The length, width, and height of the mixer chip are 13, 12.7, and 9 mm, respectively. The performance of the mixer was tested for different actuation frequencies and pneumatic pressures. The completed 3D-printed mixer was successfully applied to the colorimetric detection of for a concentration range from 102 to 108 cfu mL-1 using an enzyme-linked immunosorbent assay. The experimental results showed that the microfluidic mixer could enhance the mixing efficiency of the fluidic sample through pneumatically actuated diaphragms. In addition, the mixer could accelerate the color development caused by target , and the observed color changes could be discriminated within 5 min by naked eye. The present work will contribute to the development and optimization of a prototype for rapid detection of in food samples. It provides an effective technical approach to realize the fabrication of low-cost microfluidic chips for efficient reagent mixing in microscale biochemical detection systems. Keywords: 3D printing, Listeria monocytogenes, Microfluidic mixer, Rapid detection.

Fabrication of Tunable Silk Materials Through Microfluidic Mixers

2016 ◽  
Author(s):  
Joseph R. Nalbach ◽  
Dave Jao ◽  
Douglas G. Petro ◽  
Kyle M. Raudenbush ◽  
Shibbir Ahmad ◽  
...  
Keyword(s):  
Soft Lithography ◽  
Mixing Processes ◽  
Microfluidic Mixing ◽  
Microfluidic Mixer ◽  
Continuous Mixing ◽  
Mixing Performance ◽  
3D Printed ◽  
Distribution Uniformity ◽  
Mixing Patterns ◽  
Microfluidic Mixers

A common method to precisely control the material properties is to evenly distribute functional nanomaterials within the substrate. For example, it is possible to mix a silk solution and nanomaterials together to form one tuned silk sample. However, the nanomaterials are likely to aggregate in the traditional manual mixing processes. Here we report a pilot study of utilizing specific microfluidic mixing designs to achieve a uniform nanomaterial distribution with minimal aggregation. Mixing patterns are created based on classic designs and then validated by experimental results. The devices are fabricated on polydimethylsiloxane (PDMS) using 3D printed molds and soft lithography for rapid replication. The initial mixing performance is validated through the mixing of two solutions with colored dyes. The microfluidic mixer designs are further analyzed by creating silk-based film samples. The cured film is inspected with scanning electron microscopy (SEM) to reveal the distribution uniformity of the dye particles within the silk material matrix. Our preliminary results show that the microfluidic mixing produces uniform distribution of dye particles. Because the microfluidic device can be used as a continuous mixing tool, we believe it will provide a powerful platform for better preparation of silk materials. By using different types of nanomaterials such as graphite (demonstrated in this study), graphene, carbon nanotubes, and magnetic nanoparticles, the resulting silk samples can be fine-tuned with desired electrical, mechanical, and magnetic properties.

A G-quadruplex DNAzyme-based LAMP biosensing platform for a novel colorimetric detection ofListeria monocytogenes

2018 ◽  
Vol 10 (8) ◽  
pp. 848-854 ◽  
Author(s):  
Zhanmin Liu ◽  
Chenhui Yao ◽  
Yanming Wang ◽  
Cuiyun Yang
Keyword(s):  
Listeria Monocytogenes ◽  
Visual Detection ◽  
Colorimetric Detection ◽  
G Quadruplex ◽  
Oflisteria Monocytogenes ◽  
Cascade Amplification

A LAMP-based method for the visual detection ofListeria monocytogeneshas been developed by employing DNAzyme-catalyzed cascade amplification of the colorimetric signal.

scholarly journals Automated Photonic Tuning of Silicon Microring Resonators Using a 3D-printed Microfluidic Mixer

OSA Continuum ◽  
2021 ◽  
Author(s):  
Christian Carver ◽  
Mawla Boaks ◽  
JuHang Kim ◽  
Kevin Larson ◽  
Gregory Nordin ◽  
...  
Keyword(s):  
Microring Resonators ◽  
Microfluidic Mixer ◽  
3D Printed

Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

2020 ◽  
Vol 26 (5) ◽  
pp. 430-443 ◽  
Author(s):  
Sudarat Ledlod ◽  
Supatra Areekit ◽  
Somchai Santiwatanakul ◽  
Kosum Chansiri
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Listeria Monocytogenes ◽  
Colorimetric Detection ◽  
Simultaneous Detection ◽  
Dna Aptamer ◽  
Local Market ◽  
Salmonella Spp ◽  
Meat Samples ◽  
Colorimetric Aptasensor

In this study, we successfully developed a simple and rapid method for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli using gold nanoparticles and the aptamer aptasensor. We screened 25 specific DNA aptamer candidates against these pathogens using whole-cell Systematic Evolution of Ligands by EXponential enrichment. Among them, Ap6 was selected due to its low energy minimization values of −12.25 and −27.67 kcal/mol derived from MFold and RNAFold analysis, respectively. The assay presented in this study allowed the visual colorimetric detection of labeled colloidal gold nanoparticles as well as determination of UV absorbance at 625 and 525 nm under optimized conditions. The detection limit of this aptasensor was as less as 105 CFU/ml. A random investigation of 50 meat samples, including ham and chicken sausages, collected from the local market revealed 96% accuracy, 96% specificity, and 100% sensitivity of the assay. The colorimetric aptasensor can accomplish one-step detection without pre-culture, DNA extraction, and amplification. Hence, it is an easy, rapid, specific, and qualitative assay that can be used as a point-of-care testing to directly detect multiplex foodborne pathogens.

scholarly journals Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay

Sensors ◽  
2021 ◽  
Vol 21 (23) ◽  
pp. 8044
Author(s):  
Tushar Krishnan ◽  
Hsin-Neng Wang ◽  
Tuan Vo-Dinh
Keyword(s):  
Point Of Care ◽  
Colorimetric Detection ◽  
Mobile App ◽  
Ease Of Use ◽  
Laboratory Equipment ◽  
Non Invasive ◽  
3D Printed ◽  
Mirna Detection ◽  
Detection Schemes ◽  
Detection Technologies

The detection of microRNAs (miRNAs) is emerging as a clinically important tool for the non-invasive detection of a wide variety of diseases ranging from cancers and cardiovascular illnesses to infectious diseases. Over the years, miRNA detection schemes have become accessible to clinicians, but they still require sophisticated and bulky laboratory equipment and trained personnel to operate. The exceptional computing ability and ease of use of modern smartphones coupled with fieldable optical detection technologies can provide a useful and portable alternative to these laboratory systems. Herein, we present the development of a smartphone-based device called Krometriks, which is capable of simple and rapid colorimetric detection of microRNA (miRNAs) using a nanoparticle-based assay. The device consists of a smartphone, a 3D printed accessory, and a custom-built dedicated mobile app. We illustrate the utility of Krometriks for the detection of an important miRNA disease biomarker, miR-21, using a nanoplasmonics-based assay developed by our group. We show that Krometriks can detect miRNA down to nanomolar concentrations with detection results comparable to a laboratory-based benchtop spectrophotometer. With slight changes to the accessory design, Krometriks can be made compatible with different types of smartphone models and specifications. Thus, the Krometriks device offers a practical colorimetric platform that has the potential to provide accessible and affordable miRNA diagnostics for point-of-care and field applications in low-resource settings.

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