scholarly journals Seasonal Occurrence of the Sugar Cane Woolly Aphid, Ceratovacuna lanigera(Homoptera:Aphididae), and Its Predators in Sugar Cane Fields of Okinawa Island

1992 ◽  
Vol 27 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Norio ARAKAKI
Keyword(s):  
Sugar Cane ◽  
Seasonal Occurrence ◽  
Okinawa Island ◽  
Woolly Aphid ◽  
Ceratovacuna Lanigera

A simple mass rearing method for dipha aphidivora, the pyralid predator of sugarcane woolly aphid ceratovacuna lanigera

Sugar Tech ◽  
2006 ◽  
Vol 8 (2-3) ◽  
pp. 160-165 ◽  
Author(s):  
N. Mukunthan ◽  
R. Nirmala ◽  
G. Santhalakshmi ◽  
J. Srikanth ◽  
B. Singaravelu
Keyword(s):  
Mass Rearing ◽  
Rearing Method ◽  
Woolly Aphid ◽  
Simple Mass ◽  
Ceratovacuna Lanigera

Rearing Dipha aphidivora, the pyralid predator of sugarcane woolly aphid Ceratovacuna lanigera, on its frozen host may be unfeasible

Sugar Tech ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 80-82
Author(s):  
J. Srikanth ◽  
N. Mukunthan ◽  
B. Singaravelu ◽  
N. K. Kurup ◽  
G. Santhalakshmi
Keyword(s):  
Woolly Aphid ◽  
Ceratovacuna Lanigera

scholarly journals Efficacy of Entomopathogenic Trichoderma Isolates against Sugarcane Woolly Aphid, Ceratovacuna lanigera Zehntner (Hemiptera: Aphididae)

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 2
Author(s):  
Md. Shafiqul Islam ◽  
Vijay Kumar Subbiah ◽  
Shafiquzzaman Siddiquee
Keyword(s):  
Entomopathogenic Fungi ◽  
Agricultural Soils ◽  
Insect Pest ◽  
Sugar Content ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Adult Mortality ◽  
Pathogenic Potential ◽  
Woolly Aphid ◽  
Ceratovacuna Lanigera

Ceratovacuna lanigera Zehntner (Hemiptera: Aphididae) is a destructive insect pest of sugarcane that is responsible for reducing the quality, yield, and sugar content. Ecofriendly methods of pest control, such as the use of biological control, is encouraged since it does not exhibit the harmful effects that are usually seen when using chemical-based pesticides. Here, we evaluated the efficacy of several entomopathogenic fungi isolates against C. lanigera under laboratory conditions. A total of seven isolates were obtained from agricultural soils that were collected from various locations from Sabah, Malaysia, using the insect bait method. Among the seven, four isolates (TMS114, TMS623, TMS628, and TMS707) were positively identified as T. harzianum (Hypocreales: Hypocreaceae), two isolates (TMS45, TMS321) as T. asperellum (Hypocreales: Hypocreaceae), and one isolate (TMS633) as T. hamatum (Hypocreales: Hypocreaceae) based on a combination of morphological characteristics and molecular data using ITS 1-5.8 S-ITS 2 regions of the rDNA. Four different concentrations (1 × 105, 1 × 106, 1 × 107, and 1 × 108 mL−1) of conidia suspensions of the isolates were applied separately on nymphs and adults of C. lanigera. The results showed that mortality in both nymphs and adults was dependent on the conidia concentration and exposure time. Among the four concentrations, the highest mortality of C. lanigera was found to range from 59.99 to 75.70% for nymphs after 72 h and from 57.45 to 72.31% for adults after 6 days of treatments at the concentration of 1 × 108 conidia mL−1. Trichodermaharzianum (TMS623) showed significantly highest mortality of 75.70% for nymphs and 72.31% for adults among the seven isolates. . However, the nymph and adult mortality rates for the isolates were 70.00% & 68.00% for TMS114, 66.42% & 63.10% for TMS628, 67.85% & 65.24% for TMS707, 61.42% & 58.80% for TMS45, 59.99% & 57.45% for TMS321 and 63.56% & 60.91% for TMS633, respectively. For nymph mortality, T. harzianum (TMS623) showed a LC50 (lethal concentration) value of 6.30 × 105 conidia mL−1 and LC90 value of 3.01 × 109 conidia mL−1, respectively, and for adult mortality, the values were found of 6.16 × 105 conidia mL−1 for LC50 and 2.23 × 1010 conidia mL−1 for LC90, respectively. The lethal time (LT) values for nymph and adult mortality were found of 42.65 h and 3.89 days for LT50 and 93.32 h and 8.70 days for LT90 by T. harzianum (TMS623). Therefore, T. harzianum (TMS623) showed more pathogenic potential against C. lanigera and can be further applied to determine its efficacy under field conditions. To our best knowledge, this is the first report of T.harzianum,T.hamatum, and T.asperellum species that are used as entomopathogenic fungi against sugarcane woolly aphid, C. lanigera.

Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane

Biologia ◽  
2007 ◽  
Vol 62 (4) ◽  
Author(s):  
Dongting Zhangsun ◽  
Sulan Luo ◽  
Rukai Chen ◽  
Kexuan Tang
Keyword(s):  
Resistance Gene ◽  
Medium Composition ◽  
Marker Genes ◽  
35S Promoter ◽  
Selective Marker ◽  
Sugarcane Cultivar ◽  
Transgenic Sugarcane ◽  
Woolly Aphid ◽  
Ceratovacuna Lanigera ◽  
Screenable Marker

AbstractSix plasmids carrying a snowdrop lectin (Galanthus nivalis agglutinin, GNA) and one of three selection markers were successfully transferred into two sugarcane cultivars (FN81–745 and Badila) via Agrobacterium-mediated transformation. Agrobacterium strains LBA4404, EHA105 and A281 that harboured a super-binary vector were used for sugarcane transformation. The use of the hygromycin (Hyg) resistance gene (hpt II), phosphinothrincin (PPT) resistance gene (bar) or G418 resistance gene (npt II) as a screenable marker facilitated the initial selection of GNA transgenic sugarcane callus with different efficiencies and helped the rapid segregation of individual transformation events. All the three selective marker genes were controlled by CaMV 35S promoter, while GNA gene was controlled by promoter of RSs-1 (rice sucrose synthase-1) or Ubi (maize ubiquitin). Factors important to successful transformation mediated by Agrobacterium tumefaciens were optimized, which included concentration of A. tumefaciens, medium composition, co-cultivated methods with plant tissue, strain virulence and different selective marker genes. An efficient protocol for sugarcane transformation mediated by A. tumefaciens was established. The GNA gene has been integrated into sugarcane genome as demonstrated by PCR and Southern dot blotting detections. The preliminary results from bioassay demonstrated a significant resistance of the transgenic sugarcane plants to woolly aphid (Ceratovacuna lanigera Zehnther) indicating thus the possibility for obtaining a transgenic sugarcane cultivar with resistance to woolly aphid.

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